The miR-21 EGFP based biosensor (HeLaS3 miR-21 EGFPB) harboring a reporter for miRNA activity was generated as previously described. In brief, HeLaS3 cells were transfected with pcDNA/TO/EGFPmiR21 (Addgene, Cambridge, MA) using Lipofectamine 2000 transfection reagent and Zeocin-resistant cells were harvested for storage of cell stocks at −170°C. A549, 451Lu, A375, H460, H838, H1435, H2030, HCC1954, HEK293, HeLa, HeLaS3, MDA-MB-231, RPE, SK-MEL-5, SK-MEL-28, SK-MEL-94, SK-MEL-100, SK-MES-1 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA). JIMT-1 cell line was purchased from AddexBio (San Diego, CA). LK-2 cell line was purchased from RIKEN BioResource Center (Japan). The isogenic DICER1 knockout pairs in DLD1, HCT116, and RKO cell lines were purchased from Horizon Discovery (Cambridge, United Kingdom). All cell lines were cultured at 37°C with 5% CO2-95% air; in addition, supplies were from Thermo Fisher Scientific (Waltham, MA) and Sigma-Aldrich (St Louis, MO).
Lk 2 cell line
Manufactured by RIKEN BioResource Center
Sourced in Japan
The LK-2 cell line is an immortalized human lung cancer cell line derived from a lung adenocarcinoma. It is maintained in culture and can be used for various research purposes, such as studying the biology and behavior of lung cancer cells.
Automatically generated - may contain errors
Lab products found in correlation
Jimt1 cell line, by Addexbio (1 mentions)
Dld 1, by Horizon Discovery (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Sk mel 28, by American Type Culture Collection (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
H1435, by American Type Culture Collection (1 mentions)
Sk mel 5, by American Type Culture Collection (1 mentions)
Sk mes 1, by American Type Culture Collection (1 mentions)
Hct116, by Horizon Discovery (1 mentions)
Hcc1954, by American Type Culture Collection (1 mentions)
Hela s3, by American Type Culture Collection (1 mentions)
H2030, by American Type Culture Collection (1 mentions)
Hydrocortisone, by Nacalai Tesque (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
Y 27632, by Nacalai Tesque (1 mentions)
2 protocols using lk 2 cell line
1
HeLaS3 miR-21 EGFPB Biosensor Generation
2
Maintaining LSCC PDX Cell Lines
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The cell lines used were the three LSCC PDX cell lines (MCC121c, MCC138c, and MCC148c) described above and another LSCC-derived LK-2 cell line (purchased from RIKEN BioResource Center, Japan). The three PDX-cell lines were maintained in vitro in DMEM supplemented with 10% fetal bovine serum (FBS), 0.4 μg/ml hydrocortisone, 2.5 μM Y-27632, 100 unit/ml penicillin and 100 μg/ml streptomycin (Nakalai Tesque, Japan). The LK-2 cell line was maintained in RPMI medium supplemented with 10% fetal FBS and 1% penicillin-streptomycin. The three PDX cell lines were passaged at least 10 times in vitro before being used in experiments.
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