Advia centaur xpt 2000

WBC and HGB were determined using laser flow cytometry and laminar flow impedance in a ProCyte DX haematology analyser from IDEXX Laboratories Inc. Serum samples were further processed in the investigation laboratory (LABOKLIN). T3 and T4 were measured with a chemiluminescence assay (LIA), T3 by ADVIA Centaur XPT 2000 (Siemens), and T4 by Immulite 2000 (Siemens). Vit. E analysis was performed by high‐performance liquid chromatography (HPLC) in an HPLC LC‐20 (Shimadzu). An atomic absorption spectrometry (AAS) Zenit 650 P (Analytik Jena GmbH), which measures with AAS, was used for the selenium analyses. The concentration of SOD was determined using a manual enzymatic reaction with materials from the manufacturer, Cayman Chemical. All other blood chemical parameters were measured photometrically or potentiometrically in a cobas 8000 (Roche).

Hematology was assessed using laser flow cytometry and laminar flow impedance in a ProCyte DX hematology analyzer from IDEXX Laboratories Inc. (Westbrook, ME, USA). Blinded serum samples were further processed in the investigation laboratory (LABOKLIN, Bad Kissingen, Germany). cTnI was measured through a chemiluminescence assay (LIA) using an ADVIA Centaur XPT 2000 (Siemens, Munich, Germany). All other blood chemistry parameters were measured photometrically (PHO) or potentiometrically (POT) with a Cobas 8000 analyzer (Roche, Basel, Switzerland).