Cell apoptosis was analyzed by flow cytometry following the annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit II (BD Biosciences, San Jose, CA, USA) and propidium iodide (PI) staining according to the manufacturer’s instructions. Briefly, cells were collected, washed twice with cold phosphate-buffered saline (PBS), and resuspended in binding buffer. 100 μL of cell suspension (2 × 105 cells) was transferred to a 96-well plate, and incubated with 10 μL of Annexin V-FITC and 5 μL of propidium iodide (PI) for 15 min at room temperature in the dark. The apoptotic cells, including FITC-positive and PI-negative cells (early apoptotic), were detected with a FACS flow cytometer (BD Biosciences).
Fitc apoptosis detection kit 2
Manufactured by BD
Sourced in United States
The FITC Apoptosis Detection Kit II is a laboratory product that detects apoptosis, a type of programmed cell death, using fluorescent labeling. The kit provides the necessary reagents and protocols to identify and quantify apoptotic cells.
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Lab products found in correlation
2 protocols using fitc apoptosis detection kit 2
1
Annexin V-FITC and PI Apoptosis Assay
2
Quantifying Apoptosis in Virus-Infected Cells
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72 h after viral infection, cells were seeded in 6-well plates at 1 × 105 cells per well in M201 medium and were cultured for 48 h. The cells were collected and measured for apoptosis using flow cytometry (FACS Calibur, BD) after treatment of Annexin V: FITC Apoptosis Detection Kit II (Cat: 556570, BD). Cells were made into suspensions by trypsinization and washed with dPBS and suspended in 500 μL binding buffer and added with 5 μL Annexin V-FITC in dark for 10 m. Cells were then stained with 5 μL propidium iodide for 5 m. Apoptosis was analyzed on BD-FACS Calibur using FITC (FL1) channel and PI (FL2) channel at an excitation wavelength at 488 nm.
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