The proteins were collected from the indicated cells with lysis buffer (Beyotime), and the concentration was analyzed with a BCA protein assay kit (Beyotime). The proteins were separated on a 10% or 12% SDS-PAGE gel and subsequently transferred to PVDF membranes (Millipore, United States). The membranes were then blocked with 5% nonfat milk for at least 60 min at room temperature. After incubating with primary antibodies overnight at 4 °C, the membranes were incubated with secondary antibodies and washed three times with PBS-T. The proteins on the membranes were detected using chemiluminescence (Thermo Fisher Scientific). The antibody against UBE2T (SAB1104968) was from Sigma. The antibodies against GAPDH (ab9485), ITGA2 (ab133557), FOSL1 (ab124722), PTGS2 (ab15191), IL1B (ab9722), and BMP6 (ab155963) were from Abcam. All the secondary antibodies were from Santa Cruz.
Ab124722
Manufactured by Abcam
Sourced in United Kingdom
Ab124722 is a primary antibody that recognizes the Neurofilament Heavy Polypeptide. It is suitable for use in Western blotting, Immunohistochemistry, and Immunocytochemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab155963, by Abcam (1 mentions)
Ab133557, by Abcam (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Ab9485, by Abcam (1 mentions)
Ab9722, by Abcam (1 mentions)
Lysis buffer, by Beyotime (1 mentions)
Ab11959, by Abcam (1 mentions)
Ab3298, by Abcam (1 mentions)
Ab119944, by Abcam (1 mentions)
Anti β actin a5441, by Merck Group (1 mentions)
2 protocols using ab124722
1
Protein Expression Analysis Protocol
2
Antibodies for Transcription Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies specific for c-Jun (5B1, ab119944), JunD (EPR6520, ab134067), JunB (EPR6518, ab128878), c-Fos (2G2, ab129361), FosB (83B1138, ab11959), Fra-1 (EP4711, ab124722), Fra-2 (EPR4713(2), ab124830), anti-mitochondria [MTCO2] (ab3298) and Histone H3 (1791, ab1791) were obtained from Abcam (Cambridge, UK). Anti-Parkin (Prk8) (4211), -c-Fos (9F6, 2250) and -phosphorylated c-Jun (Ser63) II (9261) obtained from Cell Signalling Technology (Hertfordshire, UK). Anti-β-actin (A5441) was obtained from Sigma. Horseradish-peroxidase anti-mouse (P0260) and anti-rabbit (P0448) secondary antibodies were supplied by Dako (Stockport, UK). Highly-cross adsorbed (H+L) Alexa Fluor 488 anti-mouse (A11029) and Alexa Fluor 594 anti-rabbit (A11037) secondary antibodies from Molecular Probes, Invitrogen.
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