Pex14 sirna

Manufactured by Eurofins

PEX14 siRNA is a small interfering RNA (siRNA) targeting the PEX14 gene. PEX14 is a peroxisomal membrane protein that plays a crucial role in peroxisome biogenesis and function. The PEX14 siRNA can be used to downregulate the expression of the PEX14 gene in research applications.

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Lab products found in correlation

Sigenome non targeting sirna control pool, by Horizon Discovery (2 mentions) Pex5 sirna trifecta kit, by Integrated DNA Technologies (2 mentions)

Close spelling variants of this product

SiRNAs

2 protocols using pex14 sirna

Targeted Knockdown of PEX Proteins

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The plasmids and antibodies used in this study are detailed in Table S1, Table S2, respectively. PEX14 siRNA (GAACUCAAGUCCGAAAUUA) [31 ] was generated by Eurofins as a 21-mer siRNA with 3′ dTdT overhangs. PEX5 siRNA (TriFECTa kit) was obtained from Integrated DNA Technologies. siGENOME Non-Targeting siRNA Control Pool (Dharmacon) was used as a control.

Passmore J.B., Carmichael R.E., Schrader T.A., Godinho L.F., Ferdinandusse S., Lismont C., Wang Y., Hacker C., Islinger M., Fransen M., Richards D.M., Freisinger P, & Schrader M. (2020). Mitochondrial fission factor (MFF) is a critical regulator of peroxisome maturation. Biochimica et Biophysica Acta. Molecular Cell Research, 1867(7), 118709.

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Plasmid and Antibody Protocols

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The plasmids and antibodies used in this study are detailed in Tables S1 andS2, respectively. PEX14 siRNA (GAACUCAAGUCCGAAAUUA) (Lee et al. 2017) (link) and MFF siRNA (GACCAGCAGAUCUUGACCU) (Long et al. 2013) (link) were generated by Eurofins as 21-mer siRNAs with 3' dTdT overhangs. PEX5 siRNA (TriFECTa kit) was obtained from Integrated DNA Technologies. siGENOME Non-Targeting siRNA Control Pool (Dharmacon) and siMAX Non Specific siRNA Control 47% GC (AGGUAGUGUAAUCGCCUUG-TT, Eurofins) were used as controls.

Passmore J.B., Carmichael R.E., Schrader T.A., Godinho L.F., Ferdinandusse S., Lismont C., Wang Y., Hacker C., Islinger M., Fransen M., Richards D.M., Freisinger P., & Schrader M. (2020). Mitochondrial fission factor (MFF) is a critical regulator of peroxisome maturation.

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