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Las x platform

Manufactured by Leica
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The LAS X platform is a comprehensive software suite developed by Leica Microsystems for their microscope systems. It provides a unified interface for controlling various microscope hardware components and acquisition of digital images. The core function of the LAS X platform is to enable seamless integration and operation of Leica's microscopy solutions.

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Lab products found in correlation

Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Tmre mitochondrial membrane potential assay kit, by Abcam (1 mentions)

Close spelling variants of this product

LAS X 3.5.5.19976 software platform LAS X Life Science Microscope Software Platform LAS X software platform LAS X

2 protocols using las x platform

1

Immunocytochemistry of Cells on Coverslips

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Cells plated on poly-d-lysine coated glass coverslips fixed 1 h in 4% (w/v) paraformaldehyde (Electron Microscopy Sciences, EMS), washed in PBS (6×, 5 min/wash), blocked 1 h (PBS, 0.8% Triton X-100, 10% normal goat serum), incubated overnight with antibody. Cells washed in PBS (6×, 5 min/wash) and goat anti-rabbit and goat anti-mouse secondary [Ig-Alexa-488 (green) or Ig-Alexa-555 (red), Invitrogen] added. Cells washed in PBS (6×, 5 min/wash), coverslips mounted on slides (Immuno Mount DAPI and DABCO Mounting Media, EMS), and fluorescence visualized (Leica SP8) and images prepared (LAS X platform, Leica).
Kallay L., Keskin H., Ross A., Rupji M., Moody O.A., Wang X., Li G., Ahmed T., Rashid F., Stephen M.R., Cottrill K.A., Nuckols T.A., Xu M., Martinson D.E., Tranghese F., Pei Y., Cook J.M., Kowalski J., Taylor M.D., Jenkins A., Pomeranz Krummel D.A, & Sengupta S. (2019). Modulating native GABAA receptors in medulloblastoma with positive allosteric benzodiazepine-derivatives induces cell death. Journal of Neuro-Oncology, 142(3), 411-422.
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2

Mitochondrial Membrane Potential Assay

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Mitochondrial membrane potential was measured using the TMRE Mitochondrial Membrane Potential Assay Kit (Abcam). D283 cells were treated with drug or control solutions (10 min, 37 °C), 50 nM TMRE added (20 min, 37 °C), and TMRE fluorescence visualized (Leica SP8) and quantified (LAS X platform, Leica).
Kallay L., Keskin H., Ross A., Rupji M., Moody O.A., Wang X., Li G., Ahmed T., Rashid F., Stephen M.R., Cottrill K.A., Nuckols T.A., Xu M., Martinson D.E., Tranghese F., Pei Y., Cook J.M., Kowalski J., Taylor M.D., Jenkins A., Pomeranz Krummel D.A, & Sengupta S. (2019). Modulating native GABAA receptors in medulloblastoma with positive allosteric benzodiazepine-derivatives induces cell death. Journal of Neuro-Oncology, 142(3), 411-422.
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