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Halt proteases and phosphate inhibitor cocktail

Manufactured by Thermo Fisher Scientific
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Halt proteases and phosphate inhibitor cocktail is a solution designed to inhibit the activity of proteases and phosphatases during protein extraction and purification. Its core function is to prevent the degradation of proteins and the dephosphorylation of phosphoproteins.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Fastprep 24, by MP Biomedicals (2 mentions)

Close spelling variants of this product

Protease inhibitor cocktail (1883 citations) Protease inhibitors (1135 citations) Halt protease inhibitor (338 citations) Halt Protease (66 citations) HALT inhibitors (5 citations) HALTTM (3 citations) 1x Halt Cocktail protease (2 citations) Proteases inhibitor cocktail (2 citations) Phosphate inhibitor cocktail (2 citations) Protease cocktails (2 citations)

2 protocols using halt proteases and phosphate inhibitor cocktail

1

Liver Homogenization and Protein Quantification

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Livers were collected and homogenized in radioimmunoprecipitation assay buffer supplemented with Halt proteases and phosphate inhibitor cocktail (Pierce Biotechnology, Rockford, IL), using lysing matrix tubes and FastPrep-24 (MP Biomedicals, Santa Ana, CA). Homogenates were centrifuged at 12,000 rpm, the supernatants collected, and the total protein content measured using BCA Protein Assay kits (Pierce Biotechnology). After normalizing the protein content, we assayed the tissue homogenates for MAdCAM-1 and CCL25 protein by ELISA (R&D Systems).
Omenetti S., Brogi M., Goodman W.A., Croniger C.M., Eid S., Huang A.Y., Laffi G., Roskams T., Cominelli F., Pinzani M, & Pizarro T.T. (2015). Dysregulated Intrahepatic CD4+ T-Cell Activation Drives Liver Inflammation in Ileitis-Prone SAMP1/YitFc Mice. Cellular and Molecular Gastroenterology and Hepatology, 1(4), 406-419.
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2

Liver Protein Extraction and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Livers were collected and homogenized in RIPA buffer supplemented with Halt proteases and phosphate inhibitor cocktail (Pierce Biotechnology, Rockford, IL), using Lysing Matrix tubes and FastPrep®-24 (MP Biomedicals, Santa Ana, CA). Homogenates were centrifuged at 12,000 rpm, supernatants collected, and total protein content measured using BCA Protein Assay kits (Pierce Biotechnology). After normalizing protein content, tissue homogenates were assayed for MAdCAM-1 and CCL25 protein by ELISA (R&D System).
Omenetti S., Brogi M., Goodman W.A., Croniger C.M., Eid S., Huang A.Y., Laffi G., Roskams T., Cominelli F., Pinzani M, & Pizarro T.T. (2015). Dysregulated Intrahepatic CD4+ T-Cell Activation Drives Liver Inflammation in Ileitis-Prone SAMP1/YitFc Mice. Cellular and Molecular Gastroenterology and Hepatology, 1(4), 406-419.
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