1 2 dioleoyl sn glycero 3 phosphoethanolamine n lissamine rhodamine b sulfonyl rhodamine pe

Manufactured by Avanti Polar Lipids

Sourced in United States

1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhodamine-PE) is a fluorescent phospholipid used as a labeling reagent. It consists of a phosphoethanolamine headgroup conjugated to a lissamine rhodamine B sulfonyl fluorophore. This compound can be used to label lipid bilayers and other membrane-related structures.

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Lab products found in correlation

Avance 1 nmr spectrometer, by Bruker (1 mentions) Calcein, by Merck Group (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine popc, by Avanti Polar Lipids (1 mentions) Sucrose, by Merck Group (1 mentions) Topspin 4, by Bruker (1 mentions) Model cary eclipse, by Agilent Technologies (1 mentions) Reduced triton x 100, by Merck Group (1 mentions)

2 protocols using 1 2 dioleoyl sn glycero 3 phosphoethanolamine n lissamine rhodamine b sulfonyl rhodamine pe

Polymer Vesicle Characterization Protocol

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All organics solvents and chemicals were purchased from Sigma-Aldrich (Sigma-Aldrich Chimie, Saint Quentin Fallavier, France) and Thermo Scientific (Waltham, MA, USA). 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhodamine-PE) were obtained from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Poly(dimethylsiloxane)-nitrobenzoxadiazole (PDMS-NBD) and poly(dimethylsiloxane)-block-poly(ethylene oxide) (PDMS₃₆-b-PEO₂₃, M_n 4000 g.mol^-1) were synthesized as previously described [20 (link)]. This copolymer is known to spontaneously form polymersomes with a membrane thickness of 9.9 ± 1.6 nm. Cationic switchable lipid (CSL) was synthesized as previously described [16 (link)]. Sucrose, calcein and all other chemicals were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). ¹H Nuclear Magnetic Resonance spectra were acquired at 298 K on a Bruker Avance I NMR spectrometer operating at 400 MHz, using trimethylsilane (TMS) as the internal standard and deuterated chloroform as solvent. Data were treated with TopSpin 4.0.7 (Bruker BioSpin, Wissembourg, France).

Passos Gibson V., Fauquignon M., Ibarboure E., Leblond Chain J, & Le Meins J.F. (2020). Switchable Lipid Provides pH-Sensitive Properties to Lipid and Hybrid Polymer/Lipid Membranes. Polymers, 12(3), 637.

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Fusion Dynamics of v-discs with t-discs and t-liposomes

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To determine whether v-discs fuse with either t-discs or t-liposomes, 1.5 molar % of each 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (rhodamine-PE) (Avanti Polar Lipids) were included in v-discs. Both t-discs and t-liposomes were prepared with unlabeled phospholipids to dilute NBD-PE and rhodamine-PE when fusion occurred. The molar ratios of lipid:SNARE for nanodiscs and t-liposomes were 160:0.5 and 200:1, respectively. The v-discs were then mixed with 9× lipid molar excess of either t-discs or t-liposomes at 37 °C in a fusion buffer (10 mM HEPES, 100 mM KCl, pH 7.4). The reaction solution contained total 0.5 mM lipids. The NBD fluorescence intensity was measured at excitation and emission wavelengths of 465 and 530 nm, respectively with a fluorescence spectrophotometer (Cary Eclipse model, Varian). The maximum fluorescence intensity was obtained by adding 0.1% reduced Triton X-100 (Sigma).

Shin J., Lou X., Kweon D.H, & Shin Y.K. (2014). Multiple conformations of a single SNAREpin between two nanodisc membranes reveal diverse pre-fusion states. The Biochemical journal, 459(1), 95-102.

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