Ubc13

Manufactured by Zymo Research

Ubc13 is a highly specific and reliable enzyme from Zymo Research, a leading provider of innovative molecular biology tools and reagents. This enzyme plays a crucial role in the ubiquitination process, a fundamental cellular mechanism responsible for the regulation and degradation of various proteins. Ubc13 functions as an E2 ubiquitin-conjugating enzyme, facilitating the transfer of ubiquitin molecules to target proteins, thereby marking them for further cellular processing. Researchers and scientists across diverse fields can utilize Ubc13 to investigate and study the intricate dynamics of protein regulation and turnover within living systems.

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Lab products found in correlation

Ube2s, by Cell Signaling Technology (2 mentions) Brca1, by Santa Cruz Biotechnology (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Mccoy s 5a, by Corning (1 mentions) Lamin a, by Merck Group (1 mentions) Ubiquitin, by Santa Cruz Biotechnology (1 mentions) Rap80, by Fortis Life Sciences (1 mentions) Rad18, by Abcam (1 mentions) Prpa32, by Fortis Life Sciences (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions) γ tubulin, by Merck Group (1 mentions) Cyclin b1, by Santa Cruz Biotechnology (1 mentions)

2 protocols using ubc13

Cell Culture and Antibody Usage

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The human U2OS cell line was grown in McCoy's 5A with L-glutamine medium (Cellgro, Corning) supplemented with 10% FBS (GenDEPOT) and 1% penicillin/streptomycin (Gibco). The 293T cell line was grown in DMEM (Cellgro, Corning) with 4.5 g/L glucose, L-glutamine, and sodium pyruvate medium supplemented with 10% FBS and 1% penicillin/streptomycin. Antibodies used are: Cezanne (Santa Cruz, sc-514402), RAP80 (Bethyl Laboratories, A300-763A), Abraxas (homemade), HA (Cell Signaling Technology, 3724s, 2367s), GFP (Invitrogen, A11122, A11120), K63 (EMD Millipore, 05-1308), ubiquitin (Santa Cruz, sc-8017), Ubc13 (Zymed, 37-1100), Ube2S (Cell Signaling Technology, 11878s), Lamin A (Sigma, L1293), GAPDH (Invitrogen, MA5-15738), BRCA1 (Santa Cruz, sc-6954), 53BP1 (Upstate, 05-726), γH2AX (Upstate, 05-636 JBW103), Rad18 (Abcam, ab188235), and pRPA32 (Bethyl, A300-245).

Wu X., Liu S., Sagum C., Chen J., Singh R., Chaturvedi A., Horton J.R., Kashyap T.R., Fushman D., Cheng X., Bedford M.T, & Wang B. (2019). Crosstalk between Lys63- and Lys11-polyubiquitin signaling at DNA damage sites is driven by Cezanne. Genes & Development, 33(23-24), 1702-1717.

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Antibodies Used in DNA Damage Response Study

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Antibodies used in this study are BRCA1 (Santa Cruz), γ-tubulin (Sigma); HA (Cell Signaling), γH2AX (Upstate), Ube2S (Cell Signaling, Abcam), Ube2C (Sigma), Flag (Sigma), H2A (Abcam), H2AX (Abcam), H3 (Abcam), phospho H3 (Upstate), 53BP1 (Upstate), ubH2A (Abcam), GFP (Invitrogen), FK2 (Biomol), Cyclin B1 (SantaCruz), ATM (Cell Signaling), ATR (SantaCruz sc-1887), Ubc13 (Zymed Laboratories), Rap80 (Bethyl A300-763A), Rpb1 (SantaCruz sc-899), PCNA (Santa Cruz), OTUD7B (Santa Cruz sc-514402). The rabbit anti-Abraxas antibodies was generated as previously described (Wang et al., 2007 (link)).

Paul A, & Wang B. (2017). RNF8- and Ube2S-dependent ubiquitin lysine 11-linkage modification in response to DNA damage. Molecular cell, 66(4), 458-472.e5.

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