Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti gfp

Manufactured by Fortrea
Visit Supplier

Rabbit anti-GFP is a primary antibody that specifically binds to the Green Fluorescent Protein (GFP). It is commonly used in various applications such as immunohistochemistry, Western blotting, and flow cytometry to detect and visualize GFP-tagged proteins in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Mouse anti gfp, by Roche (2 mentions) Mouse anti ha, by Fortrea (2 mentions) Chicken anti gfp, by Abcam (2 mentions) Alexa fluor 405, by Thermo Fisher Scientific (1 mentions) Ab100792, by Abcam (1 mentions) Ab11805, by Abcam (1 mentions) Rabbit anti c myc, by Merck Group (1 mentions) Streptavidin alexa fluor 555, by Thermo Fisher Scientific (1 mentions) Mouse anti gfp b 2, by Santa Cruz Biotechnology (1 mentions) Anti flag m2 bead, by Merck Group (1 mentions) Anti flag m2, by Merck Group (1 mentions) Mouse anti h3k4me2, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-GFP (7 citations) Custom-made rabbit polyclonal anti-GFP antibodies (2 citations)

4 protocols using rabbit anti gfp

1

Immunofluorescence Staining with Diverse Antibodies

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used: chicken anti-HTP-3 (1:1000, (46 (link))), chicken anti-GFP (1:250, (Abcam)), mouse anti-GFP (1:500, (Roche)), rabbit anti-GFP (1:500, (47 (link))), mouse anti-HA (1:1000, (Clone 16B12; Covance)), rabbit anti-MSH-5 (1:10 000, (SDIX)), rabbit anti-RAD-51 (1:500, (48 (link))), rat anti-RAD-51 (1:200, (49 (link))), guinea pig anti-SUN-1 pS24 (1:700, (50 (link))), guinea pig anti-SUN-1 pS8(1:1000, (50 (link))), guinea pig anti-SYP-1 (1:200, (51 (link))). Secondary antibodies used were Alexa Fluor 405 (1:100), 488 (1:400), 555 (1:400), and 647 (1:200)-conjugated goat antibodies raised against the appropriate species (Life Technologies).
Yamaya K., Wang B., Memar N., Odiba A.S., Woglar A., Gartner A, & Villeneuve A.M. (2023). Disparate roles for C. elegans DNA translocase paralogs RAD-54.L and RAD-54.B in meiotic prophase germ cells. Nucleic Acids Research, 51(17), 9183-9202.
+ Open protocol
+ Expand
2

Immunostaining Characterization of Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study are as follows. Goat anti-FSTL1 (ab11805; Abcam), rabbit anti-FSTL1 (Proteintech), rabbit anti-DIAPH1 (5486; Cell Signaling Technology), rabbit anti-KSRP (A302-22A; Bethyl Laboratories), mouse anti-KRT14 (clone LLO01), mouse anti-EGF (clone 10825; R&D Systems), mouse anti-TGFβ1 (Novacastra), rabbit anti-GFP (Covance), rabbit anti-cMyc (Sigma-Aldrich), and rabbit anti-Wnt7a (ab100792; Abcam). Streptavidin Alexa Fluor 555, chicken anti-goat Alexa Fluor 488, and donkey anti-rabbit/mouse Alexa Fluor 488/555 were from Molecular Probes. Rabbit anti-Arp3 (4738), phospho (9101), and total ERK antibodies (9102) were from Cell Signaling Technology. Rabbit anti-Arpin (ABT251) was purchased from Merck Millipore. Recombinant human FSTL1 (His-tagged) expressed and purified from mammalian cells was purchased from Thermo Fisher Scientific.
Sundaram G.M., Ismail H.M., Bashir M., Muhuri M., Vaz C., Nama S., Ow G.S., Vladimirovna I.A., Ramalingam R., Burke B., Tanavde V., Kuznetsov V., Lane E.B, & Sampath P. (2017). EGF hijacks miR-198/FSTL1 wound-healing switch and steers a two-pronged pathway toward metastasis. The Journal of Experimental Medicine, 214(10), 2889-2900.
+ Open protocol
+ Expand
3

Immunoprecipitation of Krimper Protein Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dissected ovaries were lysed in NT2 buffer (50 mM Tris at pH 7.4, 150 mM NaCl, 1mM MgCl2, 0.05% Igepal, EDTA-free Complete Protease Inhibitor Cocktail). Lysate was incubated in the presence or absence of 100µg/mL RNAse A, cleared by centrifugation and incubated with anti-FLAG M2 beads (Sigma Aldrich) at 4°C for 90 min., follow ed by washing and elution in reducing SDS buffer. Antibodies used for Western Blots were rabbit anti-GFP (Covance), mouse anti-GFP B-2 (Santa Cruz Biotechnology) or anti-FLAG M2 (Sigma Aldrich) at 1:3,000 concentration. Anti-Krimper rabbit polyclonal antibody generously provided by Dr. T. Kai was used at a concentration of 1:20,000. A variant protocol for S2 cell co-immunoprecipitation and other details are provided in the Supplemental Experimental Procedures.
Webster A., Li S., Hur J.K., Wachsmuth M., Bois J.S., Perkins E.M., Patel D.J, & Aravin A.A. (2015). Aub and Ago3 are recruited to nuage through two mechanisms to form a ping-pong complex assembled by Krimper. Molecular cell, 59(4), 564-575.
+ Open protocol
+ Expand
4

Immunofluorescence Staining of Meiotic Proteins

Cited 4 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used at the indicated dilutions: Chicken anti-HTP-3 (1:500) (MacQueen et al., 2005 (link)), guinea pig anti-HTP-3 (1:500) (MacQueen et al., 2005 (link)), rabbit anti-MSH-5 (1:10000) (SDI/Novus), chicken anti-GFP (1:2000) (Abcam), mouse anti-GFP (1:500) (Roche), rabbit anti-GFP (1:500) (Yokoo et al., 2012 (link)), mouse anti-HA (1:1000) (Covance), guinea pig anti-ZHP-3 (1:500) (Bhalla et al., 2008 (link)), rabbit anti-PLK-2 (Nishi et al., 2008 (link)), guinea pig anti-SYP-1 (1:200) (MacQueen et al., 2002 (link)), rat anti-RAD-51 (1:200) (Rosu et al., 2013 (link)), rabbit anti-RPA-1 (1:500) (Lee et al., 2010 (link)), mouse anti-H3K4me2 (1:2000) (Merck), rabbit anti-SYP-1 (1:2000) (MacQueen et al., 2002 (link)) and rabbit anti-SYP-2 (1:200) (Colaiacovo et al., 2003 (link)).
Secondary antibodies conjugated to Alexa dyes 405, 488, 555 or 647, obtained from Molecular Probes, were used at 1:500 dilution. In cases where antibodies raised in mouse and guinea pig were used on the same sample, we used highly cross-absorbed goat anti-mouse secondary antibodies, obtained from Biotum (conjugated to CF488, or CF555 respectively) for secondary detection of the mouse primary antibody in order to avoid cross-reaction against antibodies raised in guinea pig.
Woglar A, & Villeneuve A.M. (2018). Dynamic Architecture of DNA Repair Complexes and the Synaptonemal Complex at Sites of Meiotic Recombination. Cell, 173(7), 1678-1691.e16.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!