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2 protocols using amyloid beta peptides

1

Mass Spectrometry Sample Preparation

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Methanol (MeOH), acetonitrile (ACN), acetic acid, and formic acid (FA) were purchased from Fisher Scientific (Pittsburgh, PA). α-cyano-4-hydroxycinnamic acid (CHCA) and 3-nitrobenzyl alcohol (3-NBA) were purchased from Sigma-Aldrich (St. Louis, MO). 2, 5-dihydroxybenzoic acid (DHB) was purchased from Acros Organics (Morris Plains, NJ). Microscope glass slides were purchased from VWR international, LLC (Radnor, PA). Conductive indium tin oxide (ITO)-coated glass slides were purchased from Bruker (Billerica, MA). Human insulin was purchased from Promega (Madison, WI, USA). Peptide standards (bradykinin, somatostatin II, angiotensin II, FMRF, FMRFamide-like peptide II (lobster)) were purchased from American Peptide Company (Sunnyvale, CA, USA). Amyloid beta peptides were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). No further purifications were performed for any reagents. All solvents used in this study were of HPLC grade. Purified water (conductivity of 18.2 MΩ·cm) was obtained from Milli-Q Reference System (Millipore Corp., Bedford, MA, USA).
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2

MALDI-TOF MS Protein and Peptide Analysis

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Methanol (MeOH), acetonitrile (ACN), acetic acid (AA), 2-nitrobenzaldehyde (NBA), and formic acid (FA) were purchased from Fisher Scientific (Pittsburgh, PA). The matrices of sinapic acid (SA) and α-cyano-4-hydroxycinnamic acid (CHCA) were purchased from Sigma-Aldrich (St. Louis, MO). Microscope glass slides were purchased from VWR international, LLC (Radnor, PA). Conductive indium tin oxide (ITO) coated glass slides were purchased from Bruker (Billerica, MA). Human insulin was purchased from Promega (Madison, WI, USA). Other protein samples (including cytochrome c from bovine heart, bovine serum albumin, human serum albumin and bovine transferrin) were purchased from Sigma-Aldrich (St. Louis, MO). Peptide standards (bradykinin, somatostatin II, angiotensin II, FMRF, FMRFamide-like peptide II (lobster)) were purchased from American Peptide Company (Sunnyvale, CA, USA). Amyloid beta peptides were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). No further purifications were performed for all reagents. All solvents used in this study were of HPLC grade. Purified water (conductivity of 18.2 MΩ.cm) was obtained from Milli-Q Reference System (Millipore Corp., Bedford, MA, USA).
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