Ht29 mtx cells

The ACQ probes, P2 (λ_abs/λ_em = 708 nm/732 nm) and P4 (λ_abs/λ_em = 651 nm/662 nm), were synthesized in the lab. Oleic acid, decanoic acid, lauric acid, myristic acid, palmitic acid, and stearic acid were purchased from Sinopharm Reagent Co., Ltd. (Shanghai, China). Cremophor EL, choline hydroxide, linOleic acid, and linolenic acid were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Paclitaxel and docetaxel were from Meilun Biotechnology Co., Ltd. (Dalian, China). Diamidino-2-phenylindole (DAPI) for nuclear staining was purchased from Yeasen Biotech Co., Ltd. (Shanghai, China). Caco-2 and Raji cells were from National Collection of Authenticated Cell Cultures (Shanghai, China), while HT29-MTX cells were from China Center for Type Culture Collection (Wuhan, China).

Labrafac CC was a kind gift from Gattefossé (Cedex, France), while Tween-80 and PEG-400 were from SINOPHARM (Beijing, China). Neoral® was purchased from Novartis (Basel, Switzerland). DAPI (4′,6-diamidino-2-phenylindole) and optimal cutting temperature (OCT) compound was from Shanghai Yeasen Biotech (Shanghai, China) and Leica (Mannheim, Germany), respectively. DMEM, RPMI 1640 and fetal bovine serum are from Gibco Invitrogen (Carlsbad, CA, USA). Caco-2 (human colorectal adenocarcinoma cell) and Raji (human Burkitt's lymphoma cell) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), while HT29-MTX cells were from China Center for Type Culture Collection (Wuhan, China). The ACQ probes, known as P2 and P4, were synthesized in the laboratory²⁶ .
Sprague–Dawley (SD) rats (male, 200 ± 20 g) were provided by SLAC Laboratory Animal (Shanghai, China). The procedures of animal experimental were approved by the Committee on Welfare and Ethics of Experimental Animals at School of Pharmacy, Fudan University, China.