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3 protocols using anti tubulin ab11304

1

Immunohistochemistry Antibody Panel for Myogenesis

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Anti-MHC (MF20) and anti-myogenin (F5D) were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD, National Institutes of Health and maintained by The University of Iowa, Department of Biological Sciences. Anti-Cxcl14 (NBP1–31398) and anti-MyoD (NB100–56511) were from Novus Biologicals (Littleton, CO, USA). Anti-F4/80 (MCA497RT) was from Bio-Rad (Hercules, CA, USA). Anti-tubulin (ab11304) was from Abcam (Cambridge, MA, USA). Anti-p21 (sc-471:M-19) was from Santa Cruz Biotechnology (Dallas, TX, USA). All other primary antibodies were from Cell Signaling Technology (Danvers, MA, USA): anti-ERK: no. 9102; anti-pERK: No. 9106; anti-MEF2a: No. 9736. Alexa Fluor fluorescent secondary antibodies were from Thermo Fisher Scientific (Waltham, MA. USA). All other secondary antibodies were from Jackson ImmunoResearch Laboratories, (West Grove, PA, USA). Gelatin, BrdU and AraC were from Sigma-Aldrich (St. Louis, MO, USA). Recombinant Cxcl14 protein was from Novus Biologicals. U0126 and ELISA kit for detection of mouse Cxcl14 was from Thermo Scientific.
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Antibody Reagents for Cell Biology

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Antibodies for MHC (clone MF20) and myogenin (clone F5D) were obtained from the Developmental Studies Hybridoma bank (developed under the auspices of the NICHD, National Institutes of Health, and maintained by the University of Iowa, Department of Biological Sciences). Anti-Flag-BioM2 (F9291), anti-MEKK4 (M7194), and gelatin were purchased from Sigma. Anti-tubulin (ab11304) and anti-ThrRS (ab236903) were from Abcam. Antibodies for the following proteins were obtained from Cell Signaling Technology: JNK (9252), phospho-JNK (Thr183/Tyr185; 9255), MKK4 (9152), phospho-MKK4 (Ser257/Thr261; 9156), MKK7 (4172), phospho-MKK7 (Ser271/Thr275; 4171), HA tag (3724), and Axin1 (2087). Peroxidase-conjugated anti-rabbit (115-036-003) and anti-mouse (111-036-003) IgG and FITC-conjugated anti-mouse IgG (115-096-003) were from Jackson ImmunoResearch Laboratories. EdU (5-Ethynyl-2-deoxyuridine) was from Carbosynth Limited, and 5-FAM-azide was from Lumiprobe.
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3

Immunohistochemistry Antibody Panel for Myogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-MHC (MF20) and anti-myogenin (F5D) were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD, National Institutes of Health and maintained by The University of Iowa, Department of Biological Sciences. Anti-Cxcl14 (NBP1–31398) and anti-MyoD (NB100–56511) were from Novus Biologicals (Littleton, CO, USA). Anti-F4/80 (MCA497RT) was from Bio-Rad (Hercules, CA, USA). Anti-tubulin (ab11304) was from Abcam (Cambridge, MA, USA). Anti-p21 (sc-471:M-19) was from Santa Cruz Biotechnology (Dallas, TX, USA). All other primary antibodies were from Cell Signaling Technology (Danvers, MA, USA): anti-ERK: no. 9102; anti-pERK: No. 9106; anti-MEF2a: No. 9736. Alexa Fluor fluorescent secondary antibodies were from Thermo Fisher Scientific (Waltham, MA. USA). All other secondary antibodies were from Jackson ImmunoResearch Laboratories, (West Grove, PA, USA). Gelatin, BrdU and AraC were from Sigma-Aldrich (St. Louis, MO, USA). Recombinant Cxcl14 protein was from Novus Biologicals. U0126 and ELISA kit for detection of mouse Cxcl14 was from Thermo Scientific.
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