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Alexa 647 conjugated anti mouse total igg

Manufactured by Thermo Fisher Scientific
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Alexa-647 conjugated anti-mouse total IgG is a laboratory reagent used for the detection and quantification of mouse immunoglobulin G (IgG) in various biological samples. The product consists of polyclonal antibodies specific to mouse IgG that are conjugated with the fluorescent dye Alexa Fluor 647. This conjugation allows for the visualization and measurement of mouse IgG through fluorescence-based techniques, such as flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Intrastain, by Agilent Technologies (2 mentions)

Close spelling variants of this product

Anti-mouse IgG-Alexa-647 Alexa 647 anti-mouse 647 anti-mouse Alexa 647 Mouse anti-IgG

2 protocols using alexa 647 conjugated anti mouse total igg

1

Conformational Change Tracking of Viral Hemagglutinin

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
A549 cells, parental or stably expressing CD8 or NCOA7, were seeded in 6-well plates. Unmodified cells were treated with 50 nM bafilomycin A1 for 1 hr prior to infection (inhibition control). Medium was removed and cells were infected in serum-free DMEM with A/Victoria/3/75 at MOI of 30 and incubated for 1 hr at 37°C. Cells were then trypsinized, and both fixed and stained using IntraStain (Dako) with monoclonal antibody A1 (14 (link)), which is specific for HA that has undergone low pH induced conformational change (provided by Yohei Yamauchi). Alexa-647 conjugated anti-mouse total IgG (Life Technologies) was used as secondary antibody, and cells were analyzed by imaging flow cytometry (see below) using the 647 nm laser for excitation.
Doyle T., Moncorgé O., Bonaventure B., Pollpeter D., Lussignol M., Tauziet M., Apolonia L., Catanese M.T., Goujon C, & Malim M.H. (2018). The interferon inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Nature microbiology, 3(12), 1369-1376.
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2

Conformational Change Tracking of Viral Hemagglutinin

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
A549 cells, parental or stably expressing CD8 or NCOA7, were seeded in 6-well plates. Unmodified cells were treated with 50 nM bafilomycin A1 for 1 hr prior to infection (inhibition control). Medium was removed and cells were infected in serum-free DMEM with A/Victoria/3/75 at MOI of 30 and incubated for 1 hr at 37°C. Cells were then trypsinized, and both fixed and stained using IntraStain (Dako) with monoclonal antibody A1 (14 (link)), which is specific for HA that has undergone low pH induced conformational change (provided by Yohei Yamauchi). Alexa-647 conjugated anti-mouse total IgG (Life Technologies) was used as secondary antibody, and cells were analyzed by imaging flow cytometry (see below) using the 647 nm laser for excitation.
Doyle T., Moncorgé O., Bonaventure B., Pollpeter D., Lussignol M., Tauziet M., Apolonia L., Catanese M.T., Goujon C, & Malim M.H. (2018). The interferon inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Nature microbiology, 3(12), 1369-1376.
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