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Anti pkm2 4053s

Manufactured by Cell Signaling Technology
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Anti-PKM2 (#4053s) is a primary antibody produced by Cell Signaling Technology. It recognizes the pyruvate kinase M2 (PKM2) protein.

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Lab products found in correlation

Anti β actin antibody, by Bioworld Technology (3 mentions) Anti kif2c antibody, by Santa Cruz Biotechnology (3 mentions) Anti flag 8146s, by Cell Signaling Technology (2 mentions) Anti flag, by Cell Signaling Technology (1 mentions) Recombinant mouse il 23, by BioLegend (1 mentions) Anti hk2 22029 1 ap, by Proteintech (1 mentions) Anti glut1 21829 1 ap, by Proteintech (1 mentions) Pe conjugated anti mouse il 17a, by BioLegend (1 mentions) Percp anti mouse cd8a, by BioLegend (1 mentions) Pe cy7 anti mouse cd86, by BioLegend (1 mentions) Anti mouse cd3e, by BioLegend (1 mentions) Recombinant mouse il 12, by BioLegend (1 mentions) 740 y p hy p0175, by MedChemExpress (1 mentions) Recombinant mouse il 2, by BioLegend (1 mentions) Anti β actin 3700, by Cell Signaling Technology (1 mentions) Recombinant mouse il 6, by BioLegend (1 mentions) Alexafluor 647 anti mouse rat human foxp3 320014, by BioLegend (1 mentions) Fitc anti mouse cd11c, by BioLegend (1 mentions) Pe anti mouse f4 80, by BioLegend (1 mentions) Anti mouse cd28, by BioLegend (1 mentions)

4 protocols using anti pkm2 4053s

1

Protein Expression Analysis by Western Blot

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Cells were treated with pharmacological agents for various times. The cells were collected into lysis buffer. Equal amounts of total proteins (20 µg) were separated by SDS-PAGE and transferred onto a nitrocellulose membrane. The membranes were probed with the appropriate antibodies. The immunoreactivity was detected by ECL and analyzed using Image Lab software. The following were commercially obtained antibodies: the anti-PKM2 (#4053s), the anti-p62 (#8025), anti-LC3 (#12741) and anti-Flag (#8146s) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA); the anti-KIF2C antibody (#sc-81305) were obtained from Santa Cruz Biotechnology; the anti-β-actin antibody was obtained from Bioworld Technology (Atlanta, Georgia, 305, USA).
Jiang C.F., Xie Y.X., Qian Y.C., Wang M., Liu L.Z., Shu Y.Q., Bai X.M, & Jiang B.H. (2021). TBX15/miR-152/KIF2C pathway regulates breast cancer doxorubicin resistance via promoting PKM2 ubiquitination. Cancer Cell International, 21, 542.
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2

Murine T Cell Polarization Assay

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Anti-mouse CD3e (100340), anti-mouse CD28 (102116), recombinant mouse IL-2 (575406), recombinant mouse IL-6 (575702), recombinant mouse TGF-β (763102), recombinant mouse IL-12 (577004), recombinant mouse IL-23 (589002), Brilliant Violet 421-conjugated anti-mouse CD4 (100443), PerCP anti-mouse CD8a (100731), PE-conjugated anti-mouse/human CD44 (103008), APC-conjugated anti-mouse CD62L (104412), PE/Cyanine7-conjugated anti-mouse IFN-γ (505826), Brilliant Violet 421-conjugated anti-mouse IL-17A (512321), PE-conjugated anti-mouse IL-17A (506904), Alexa Fluor® 647 anti-mouse/rat/human FOXP3 (320014), FITC anti-mouse CD11c (117306), Brilliant Violet 421™ anti-mouse I-A/I-E (107620), PE/Cy7 anti-mouse CD86 (105014), and PE anti-mouse F4/80 (123110) were purchased from Biolegend (San Diego, CA, USA). Anti-phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (4228S), anti-PI3 Kinase p85 (4292S), anti-phospho-AKT (Ser473) (D9E) XP® Rabbit mAb (4060S), anti-AKT (9272S), anti-PKM2 (4053S) and anti-β-Actin (3700S) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA); anti-PGK1 (A12686), anti-ENO1 (A11448), and anti-LDHA (A1146) antibodies were got from Abclonal (Wuhan, China); anti-HK2 (22029-1-AP) and anti-Glut1 (21829-1-AP) antibodies were obtained from Proteintech (Wuhan, China); and 740 Y-P (HY-P0175) was ordered from Medchem Express (Shanghai, China).
Zou Y., Zhang J., Sun F., Xu Q., Chen L., Luo X., Wang T., Zhou Q., Zhang S., Xiong F., Kong W., Yang P., Yu Q., Liu S, & Wang C.Y. (2024). Fluvoxamine inhibits Th1 and Th17 polarization and function by repressing glycolysis to attenuate autoimmune progression in type 1 diabetes. Molecular Medicine, 30, 23.
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3

Protein Expression Analysis in Cells

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Cells were treated with pharmacological agents for various times. The cells were collected into lysis buffer. Equal amounts of total proteins (20 µg) were separated by SDS-PAGE and transferred onto a nitrocellulose membrane. The membranes were probed with the appropriate antibodies. The immunoreactivity was detected by ECL and analyzed using Image Lab software. The following were commercially obtained antibodies: the anti-PKM2 (#4053s), the anti-p62 (#8025), anti-LC3 (#12741) and anti-Flag (#8146s) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA); the anti-KIF2C antibody (#sc-81305) were obtained from Santa Cruz Biotechnology; the anti-β-actin antibody was obtained from Bioworld Technology (Atlanta, Georgia, 305, USA).
Jiang C., Xie Y., Qian Y., Zhou Q., L L., Shu Y., Bai X., & Jiang B. (2021). TBX15/miR-152/KIF2C Pathway Regulates Breast Cancer Doxorubicin Resistance via Promoting PKM2 Ubiquitination.
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4

Protein Expression Analysis in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with pharmacological agents for various times. The cells were collected into lysis buffer. Equal amounts of total proteins (20 µg) were separated by SDS-PAGE and transferred onto a nitrocellulose membrane. The membranes were probed with the appropriate antibodies. The immunoreactivity was detected by ECL and analyzed using Image Lab software. The following were commercially obtained antibodies: the anti-PKM2 (#4053s), the anti-p62 (#8025), anti-LC3 (#12741) and anti-Flag (#8146s) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA); the anti-KIF2C antibody (#sc-81305) were obtained from Santa Cruz Biotechnology; the anti-β-actin antibody was obtained from Bioworld Technology (Atlanta, Georgia, 305, USA).
Jiang C., Xie Y., Qian Y., Wang M., L L., Shu Y., Bai X., & Jiang B. (2020). TBX15/miR-152/KIF2C Pathway Regulates Breast Cancer Doxorubicin Resistance via Preventing PKM2 Ubiquitination.
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