Rabbit anti gapdh pab

Western blotting was performed as described previously [30 (link)]. The following primary antibodies were used: mouse anti-β-actin mAb (1:5000; Sigma-Aldrich, A5441); rabbit anti-IL-1β pAb (1:1000; Abcam, ab9722); rabbit anti-TNF-α pAb (1:1000; Abcam, ab9739); rabbit anti-GAPDH pAb (1:5000; Proteintech, 10494-1-AP); rabbit anti-alpha-synuclein pAb (1:2000; Cell Signaling, 2628); rabbit anti-TNFR2 pAb (1:1000; Beyotime, AF8199); rabbit anti-HA tag pAb (1: 1000; Abcam, ab9110); mouse anti-FLAG tag mAb (1: 5000; Sigma-Aldrich, F1804); The membrane was washed and incubated for 2 h at room temperature with the corresponding secondary antibodies: (a) HRP-conjugated goat anti-rabbit IgG (1:10,000; Jackson ImmunoResearch Laboratories, 115-035-003); (b) HRP-conjugated goat anti-mouse IgG (1:10,000; Jackson ImmunoResearch Laboratories, 115-035-003). Peroxidase activity was detected with SuperSignal WestPico chemiluminescent substrate (Pierce Biotechnology) and visualized and digitized with ImageQuant (LAS-4000, Fujifilm, Japan). Optical densities of bands were analyzed by using ImageReader software (Fujifilm, Japan). Protein levels, quantified by computer analysis as the ratio between each immunoreactive band and the levels of β-actin or GAPDH, were expressed as a percentage of vehicle-treated control.

The following primary antibodies were purchased from commercial sources: rabbit anti-PB2 polyclonal antibody (PAb) (GeneTex), rabbit anti-PB1 PAb (GeneTex), rabbit anti-PA PAb (GeneTex), rabbit anti-ANP32A monoclonal antibody (MAb) (Cell Signaling Technology), rabbit anti-GAPDH PAb (Proteintech), mouse anti-actin MAb (Santa Cruz), and mouse anti-GST MAb (GenScript Biotech). The secondary antibodies DyLight 800 goat anti-mouse IgG(H+L) and DyLight 800 goat anti-rabbit IgG(H+L) (KPL) were used for Western blotting.