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Egm 2 without hydrocortisone

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EGM-2 without hydrocortisone is a cell culture medium used for the growth and expansion of endothelial cells. It provides the necessary nutrients and growth factors to support the in vitro culture of endothelial cells.

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Lab products found in correlation

Cd31 coated magnetic beads, by Thermo Fisher Scientific (1 mentions) Gelatin coated plates, by BD (1 mentions) Glutamine penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

EGM-2 Hydrocortisone

2 protocols using egm 2 without hydrocortisone

1

ECFC Isolation and Expansion Protocol

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The study protocol was approved by the institutional review board of Duksung Women's University (IRB No. 2017-002-001). ECFCs were isolated from the adherent mononuclear cell (MNC) fraction of human peripheral blood using CD31-coated magnetic beads (Invitrogen, MA, USA) as described previously [10 (link)]. The isolated ECFCs were expanded on 1% gelatin-coated plates (BD Biosciences, NJ, USA) using endothelial growth medium-2 (EGM-2 without hydrocortisone; Lonza, MD, USA) supplemented with 10% fetal bovine serum (FBS; Atlas Biologicals, CO, USA) and 1% glutamine-penicillin-streptomycin (GPS; Gibco, MA, USA). ECFCs between passages 7 and 10 were used for all experiments.
Lee H, & Kang K.T. (2018). Advanced tube formation assay using human endothelial colony forming cells for in vitro evaluation of angiogenesis. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 22(6), 705-712.
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2

Isolation and Expansion of ECFC and MSC from Human Tissues

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Human umbilical cord blood was obtained from the Brigham and Women’s Hospital in accordance with an Institutional Review Board-approved protocol. Endothelial colony forming cells (ECFC) were isolated from the adherent mononuclear cells (MNC) fraction as described (18 (link)). ECFC were expanded on fibronectin (FN)-coated plates (1 μg/cm2; Millipore, MA) using EGM-2 (without hydrocortisone; Lonza, MD) supplemented with 20 % FBS (Hyclone, UT). Mesenchymal stem cells (MSC) were isolated from the MNC fraction of human adult bone marrow as previously described (18 (link)). ECFC and MSC have a classic phenotype with positive CD31 and CD90 expression respectively, while the leukocyte marker CD45 was not detected (supplemental Figure 1).
Smadja D.M., Levy M., Huang L., Rossi E., Blandinières A., Israel-Biet D., Gaussem P, & Bischoff J. (2015). Treprostinil indirectly regulates endothelial colony forming cell angiogenic properties by increasing VEGF-A produced by mesenchymal stem cells. Thrombosis and haemostasis, 114(4), 735-747.
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