Human interleukin 4 il 4

Manufactured by Thermo Fisher Scientific

Human interleukin-4 (IL-4) is a cytokine produced by T cells, mast cells, and other cell types. It is a key regulator of immune and inflammatory responses.

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Lab products found in correlation

Histopaque, by Merck Group (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Hyclone rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharide lps, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Macs cd14 isolation kit, by Miltenyi Biotec (1 mentions)

3 protocols using human interleukin 4 il 4

Generation of Immature Dendritic Cells

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Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Histopaque; Sigma Aldrich, St. Louis, MO) from anonymous, blood bank blood (New York Blood Center). CD14+ cells were isolated immunomagnetically (Miltenyi Biotec, Auburn, CA) and cultured (0.7–1×10⁶ cells/ml) in DC media (RPMI (Life Technologies, Carlsbad, CA) containing 100units/ml of penicillin, 100g/ml streptomycin, 55μM β-mercaptoethanol) and 4% human serum AB (GemCell, Gemini Bio-Products, West Sacramento, CA)) supplemented with 500 U/ml human granulocyte-macrophage colony-stimulating factor (GM-CSF; Peprotech, Rocky Hill, NJ), 500 U/ml human interleukin-4 (IL-4; Peprotech), 1 ng/mL ciproflaxocin (Sigma Aldrich, St. Louis, MO) for 5 to 7 days at 37°C to produce immature DCs. By day 5, immature DCs expressed surface CD11c and HLA-DR, but low to no CD14 (data not shown).

Johnson J.C., Martinez O., Honko A.N., Hensley L.E., Olinger G.G, & Basler C.F. (2014). Pyridinyl imidazole inhibitors of p38 MAP kinase impair viral entry and reduce cytokine induction by Zaire ebolavirus in human dendritic cells. Antiviral research, 107, 102-109.

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Macrophage Activation by Titanium Wear Particles

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Thp1 cells were cultured at 37°C with 5% carbon dioxide in HyClone RPMI 1640 medium modified (Thermo Fisher Scientific Inc.), 10% fetal bovine serum (FBS) (Gibco, Life Technologies Corporation), and 100 mg/mL streptomycin (HyClone). TCP was sterilized and placed into 24-well plates (100 mg/well) using a small spatula and then into cultures with 500 μL/well of Thp1 culture medium. After 24 h of incubation, the culture medium was collected as the TCP extract.
Thp1 was first induced to macrophages by culture with 100 ng/mL of phorbol myristate acetate (PMA) (Sigma) at a density of 1 × 10⁶ cells/mL for 48 h. Then the culture medium was changed to culture medium containing TCP extract, 500 ng/mL lipopolysaccharide (LPS) (PeproTech), and 20 ng/mL human interleukin-4 (IL-4) (PeproTech). After incubation for 1 h, the supernatants of Thp1 cells cultured with TCP extract were collected.
Human bone marrow stem cells (HBMSCs) were obtained from patients undergoing iliac bone graft with informed written consent. The procedure was approved by the Ethics Committee at Wuhan University, China. HBMSCs were cultured in a-MEM medium (Thermo Fisher Scientific Inc.) at 37°C with 5% carbon dioxide.

Jia X., Xu H., Miron R.J., Yin C., Zhang X., Wu M, & Zhang Y. (2018). EZH1 Is Associated with TCP-Induced Bone Regeneration through Macrophage Polarization. Stem Cells International, 2018, 6310560.

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Generating Naive Human Dendritic Cells

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Human MDDCs were obtained from healthy human blood donors (New York Blood Center), following a standard protocol as previously described^{12 (link)}. Briefly, after Ficoll–Hypaque gradient centrifugation, CD14⁺ cells were isolated from the mononuclear fraction using a MACS CD14 isolation kit (Milteny Biotec) according to the manufacturer’s instructions. CD14⁺ cells were then differentiated to naive dendritic cells by incubation for 5–6 days in dendritic cell (DC) medium (RPMI supplemented with 100 U ml⁻¹ L-glutamine, 100 g ml⁻¹ penicillin–streptomycin and 1 mM sodium pyruvate) with the presence of 500 U ml⁻¹ human granulocyte–macrophage colony-stimulated factor (GM-CSF) (PeproTech), 1,000 U ml⁻¹ human interleukin 4 (IL-4) (PeproTech) and 10% FBS (Hyclone). The purity of the dendritic cells was confirmed by flow cytometry analysis and at least 99% were CD11c+, CD86low, CD83−, HLA-DRlow and CD14-cultured for 5 days at 37 °C, 5% CO₂. Fresh medium was added every 2–3 days. Blood from healthy human donors were obtained from the New York Blood Center. The samples were anonymous. This constitutes exempt research and does not require IRB review.

Aguirre S., Luthra P., Sanchez-Aparicio M.T., Maestre A.M., Patel J., Lamothe F., Fredericks A.C., Tripathi S., Zhu T., Pintado-Silva J., Webb L.G., Bernal-Rubio D., Solovyov A., Greenbaum B., Simon V., Basler C.F., Mulder L.C., García-Sastre A, & Fernandez-Sesma A. (2017). Dengue virus NS2B protein targets cGAS for degradation and prevents mitochondrial DNA sensing during infection. Nature microbiology, 2, 17037.

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