Goat anti mouse irdye 800rd

Cisplatin and celastrol (3-hydroxy-9β, 13α-dimethyl-2-oxo-24, 25, 26-trinoroleana-1(10), 3, 5, 7-tetraen-29-oic acid) were purchased from Sigma Aldrich (St. Louis, MO). CB2 agonist, JWH-015^{12 (link)} was purchased from Tocris Biosciences (R&D System, MN). Primary antibodies used are listed as follows: RGS17 (#12549-1-AP) from Proteintech group, IL. Two different myosin VIIa antibodies of two different species were used: Mouse IgG1 anti-myosin-VIIa antibody from Developmental Studies Hybridoma Bank (71-1-s), while rabbit anti-myosin-VIIa from Proteus Biosciences (71-6790) was purchased. Anti-CtBP2 (#612044) and anti-GluR2 (#MAB397) were obtained from BD Biosciences Millipore respectively. Secondary antibodies: Alexa Fluor 647 goat anti-rabbit, Alexa Fluor 488 goat anti-rabbit were purchased from Life Technologies whereas DyLight 488 and TRITC conjugated secondary antibodies were purchased from Jackson lmmuno Laboratories (West Grove, PA) were used for immunocytochemistry whereas donkey anti-rabbit IRDye 680RD, goat anti-mouse IRDye 800RD (no. 926–32,214) from LI-COR Biosciences (Lincoln, NE, USA) were used for western blots. RGS17 siRNA was obtained from Invitrogen - Thermo Fisher Scientific, USA.

H₂O₂, capsaicin, protease inhibitor, and phosphatase inhibitor cocktails 2 and 3 were purchased from Sigma-Aldrich (St. Louis, MO, United States). TNF-α cytokine was purchased from Sigma-Aldrich (St. Louis, MO, United States). Etanercept or Enbrel was purchased from Walgreens pharmacy for animal use. Various primary antibodies used were as follows: p-ERK1/2, ERK1/2, NOX3, β-actin from Santa Cruz Biotechnology (Dallas, TX, United States); TNF-α, Cox2 and iNOS from Cell Signaling Technology (Danvers, MA, United States); Alexa Fluor 488 Phalloidin from Thermo Fisher Scientific (Waltham, MA, United States). Secondary antibodies used were as follows: donkey anti-rabbit IRDye 680RD, goat anti-mouse IRDye 800RD (no. 926-32214) from LI-COR Biosciences (Lincoln, NE, United States); Alexa Fluor 488 goat anti-rabbit (no. A11008) and RNA Later were purchased from Thermo Fisher Scientific (Berkeley, MO, United States); Alexa Fluor 647 goat anti-mouse IgG1 (no. A-21240) from Molecular Probes (Eugene, OR, United States). Apoptosis was measured using ApopTag^® Red In Situ Apoptosis Detection Kit (Millipore Sigma, United States). Prolong gold anti-fade (Invitrogen) was used to mount immunohistochemistry samples for imaging.