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PEG 20000 is a type of polyethylene glycol (PEG) with an average molecular weight of 20,000 daltons. PEG 20000 is a white, waxy, and water-soluble solid at room temperature. It is commonly used as a polymer in various laboratory applications, including protein purification, cell culture, and drug delivery.

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2 protocols using peg 20000

1

Quantitative Lateral Flow Assay for TTX

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Hydroquinone (C6H6O2), chloroauric acid (AuCl4H), trisodium citrate dihydrate (C6H5Na3O7), and PEG 20000 were bought from Macklin (Shanghai, China). The NC membrane, sample pad, conjugated pad, polyvinylchloride (PVC) backing card, and absorbance pad were purchased from Jieyi Biotechnology Co., Ltd. (Shanghai, China). Latex microspheres were purchased from Bans Laboratories, Inc. (Indiana, United States). TTX (≥95% pure) was purchased from CATO Research Chemicals Inc. (Guangzhou, China), and anti-TTX-monoclonal antibodies (anti-TTX mAb) from hybridoma cell 5B9 were produced by us and stored in our lab (Ling et al., 2015 (link)). Anti-TTX mAb had no cross-reactivity against saxitoxin acetate (STX), conotoxin (CTX), brevetoxin (BTX), pectenotoxin (PTX), and microcystin (MC) (Ling et al., 2015 (link)). μ-Conotoxin KIIIA (μ-CTX KIIIA), αB-conotoxin VxXXIVA (αB-VxXXIVA-CTX), APETx_2 (κ-actitoxin-Ael2a, UniProt P61541), BDS-I (∆κ-actitoxin-Avd4a, UniProt P11494), and sea snake neurotoxins-311 (SN311) (≥85% pure) were prepared by Sangon Biotech (Shanghai) Co., Ltd. OA (≥98% pure) was purchased from Sigma-Aldrich (St, Louis, MO, United States).
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2

Synthesis of Redox-Active Materials

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Ferric nitrate nonahydrate (Fe(NO3)3·9H2O), glycerol, isopropyl alcohol, absolute ethanol, polyethylene glycol (PEG 20000), nitric acid (HNO3), sulfuric acid (H2SO4), potassium permanganate (KMnO4), hydrogen peroxide (H2O2), sulphur powder and graphite powder were bought from Macklin. Lithium iodide (LiI), lithium perchlorate (LiClO4) and tetrabutyl ammonium iodide, 4-tert-butyl pyridine, acetonitrile, were obtained from Aladdin. All the chemical-grade agents were commercially purchased without further purification.
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