The lentiviral pLenti-CMV plasmids expressing MYC-tagged WT or K274R mutant of rat MOF (a gift from Jianrong Lu) were described previously35 (link). The following homemade antibodies have been described: anti-MLL4#336 (link) and anti-UTX37 (link). Anti-MOF (A300–992A) and anti-RbBP5 (A300–109A) were from Bethyl Laboratories. Anti-H3 (ab1791), anti-H3K4me1 (ab8895), and anti-H4 (ab7311) were from Abcam. Anti-H3K4me3 (07–473), anti-H4K8ac (07–328), and anti-H4K16ac (07–329) were from Millipore. Anti-PARP1 (556362) was from BD Bioscience. Anti-MYC (sc40) was from Santa Cruz and 4OHT (H7904) was from Sigma. A 1:1000 dilution of antibodies was used for western blot.
4oht h7904
Manufactured by Merck Group
4OHT (H7904) is a selective estrogen receptor modulator (SERM) that functions as a tamoxifen analogue. It is commonly used as a tool compound in biochemical and cell biology research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti mof a300 992a, by Fortis Life Sciences (1 mentions)
Anti myc sc 40, by Santa Cruz Biotechnology (1 mentions)
Anti rbbp5 a300 109a, by Fortis Life Sciences (1 mentions)
Anti h4k16ac, by Merck Group (1 mentions)
Hcc1937, by Korean Cell Line Bank (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
Anti γ tubulin t6557, by Merck Group (1 mentions)
Ha sc 7392, by Santa Cruz Biotechnology (1 mentions)
2 protocols using 4oht h7904
1
Lentiviral Vectors Expressing Rat MOF
2
Cell Line Cultivation and Inhibitor Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HEK293, MCF-7, and MDA-MB-468 (DMEM) were purchased from ATCC (Manassas, VA). HCT116 p53 −/− cell line was provided by Dr. Vogelstein B (Johns Hopkins University). ACHN, A498, A704 (DMEM) and HCC1937 (RPMI) cells were obtained from Korea cell line bank. Other Cell lines (UMRC2; C2, UMRC2/VHL; C2V), provided by Dr. Jung, YJ (Pusan National University). Cells were maintained in DMEM. All kinds of cell lines were maintained in liquid medium containing 10% FBS and 1% antibiotics at 37°C growth chamber. General chemical inhibitors including Adriamycin (324380) and Colcemid (234109) were purchased from Calbiochem. B02 (SML0364), Estrogen (250155), Fulvestrant (I4409), Taxol (T7402), Tamoxifen (T5648) and 4-OHT (H7904) were purchased from Sigma. Antibodies against GST (sc-138), Actin (sc-1616), ER-α (sc-8002), β-tubulin (sc-9104) and HA (sc-7392) were purchased from Santa Cruz. Anti-γ-tubulin (T6557) and Myc (M5546) were provided by Sigma, anti-pVHL Ab (2738) was obtained from Cell signaling. Rad51 (05–530), BRCA1 (07–434) were purchased from Milliopore.
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