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G 6983

Manufactured by Santa Cruz Biotechnology
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Gö6983 is a lab equipment product manufactured by Santa Cruz Biotechnology. It is a selective and potent inhibitor of protein kinase C (PKC) isoforms.

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Lab products found in correlation

Ro 31 8220, by Santa Cruz Biotechnology (2 mentions) Ly294002, by Merck Group (1 mentions) Wortmannin, by Merck Group (1 mentions) Kt5720, by Merck Group (1 mentions) α amanitin, by AppliChem (1 mentions) Anti tubulin, by Abcam (1 mentions) Phorbol myristate acetate (pma), by Santa Cruz Biotechnology (1 mentions) Anti cyct1, by Santa Cruz Biotechnology (1 mentions) G 6976, by Santa Cruz Biotechnology (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Anti cdk9, by Santa Cruz Biotechnology (1 mentions) Ingenol 3 20 dibenzoate, by Santa Cruz Biotechnology (1 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions) U73122, by Merck Group (1 mentions) Kt5720, by Santa Cruz Biotechnology (1 mentions) Calphostin c, by Santa Cruz Biotechnology (1 mentions) Jh 3, by Merck Group (1 mentions)

4 protocols using g 6983

1

Pharmacological Modulation of Neuronal Signaling

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All standard reagents, except where indicated, were obtained either from Sigma-Aldrich (UK). D-(−)-2-amino-5-phosphonopentanoic acid (D-AP5), bicuculline methochloride and 2,3,-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX), 1,4-Diamino-2,3-dicyano-1,4-bis(o-aminophenylmercapto) butadiene (U0126), wortmannin and H-89 were purchased from Tocris Cookson Ltd (Bristol, UK). Gö6983 was purchased from Santa Cruz Biotechnology (Dallas, Tx, USA). Triciribine (TCBN) was purchased from Sigma-Aldrich (Dorset, UK). Stock solutions, at 1000 times the working concentration, were made up in water or in dimethylsulphoxide, and were stored in individual aliquots at −45°C. Working solutions were prepared freshly on the day of the experiment.
Wang J., He X., Guo F., Cheng X., Wang Y., Wang X., Feng Z., Vreugdenhil M, & Lu C. (2017). Multiple Kinases Involved in the Nicotinic Modulation of Gamma Oscillations in the Rat Hippocampal CA3 Area. Frontiers in Cellular Neuroscience, 11, 57.
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2

Melanoma Cell Line Inhibition Assay

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The melanoma cell line Mel Ei (150,000 cells) were cultured in six-well plates and were treated for 24 h with different inhibitors (SB431542 InvivoGen 2 μM; S3I-201 Sigma-Aldrich 20 mM; Dorsomorphin Tebi-bio 2 mM; LY-294002 Sigma-Aldrich 20 mM; Wortmannin Sigma-Aldrich 10 mM; UO126 Calbiochem 10 mM; Vemurafenib Active Biochem 100 μM; KT5720 Merck Millipore 100 nM; Bisindolylmaleimide II Santa Cruz 10 μM; Gö 6983 Santa Cruz 10 μM; Wnt Agonist Calbiochem 10 μM). For the stability assay the cells were treated with α-Amanitin (AppliChem, 5 mM). After isolation of total RNA, quantitative RT-PCR was performed to detect relative gene expression. Normalization was based on mRNA input in this experiment.
Ruedel A., Schott M., Schubert T, & Bosserhoff A.K. (2016). Robo3A and Robo3B expression is regulated via alternative promoters and mRNA stability. Cancer Cell International, 16, 71.
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3

Ingenol Compounds Modulate HIV Latency

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The natural compound ingenol-3-dodecatrienoates extracted from Euphorbiaceae and the derivate esters Ingenol A, B and C were isolated by Kyolab Laboratories (São Paulo, Brazil). Ingenol 3,20-dibenzoate, PMA, prostatin and the PKC inhibitors Gö 6976, Gö 6983 and Ro-31–8220 were obtained from Santa Cruz Biotechnology. SAHA was obtained from SelleckChem (Houston, USA). All these compounds were diluted to specific concentrations in DMEM or RPMI, and the final concentration of solvent (DMSO) was always less than 1%. We used 20 ng/ml of TNF-α (eBiosciences) as positive control of reactivation. Levels of P-TEFb components were evaluated by immunoblotting using specific antibodies anti-CDK9 and anti-Cyc T1 (Santa Cruz Biotechnologies, cat. number 484 and 10750, respectively). Anti-tubulin (Abcam, cat. number 56676) was used to normalize proteins level.
José D.P., Bartholomeeusen K., da Cunha R.D., Abreu C.M., Glinski J., da Costa T.B., Rabay A.F., Filho L.F., Dudycz L.W., Ranga U., Peterlin B.M., Pianowski L.F., Tanuri A, & Aguiar R.S. (2014). Reactivation of latent HIV-1 by new semi-synthetic ingenol esters. Virology, 0, 328-339.
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4

Inhibition of JH-III in Cell Culture

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JH-III was purchased from Sigma Aldrich and was dissolved in ethanol. In all inhibition experiments, cells were pre-incubated with inhibitors for 1 h before the addition of JH-III. Final concentrations of inhibitors in all cell-culture studies were as follows: calphostin C (Santa Cruz Biotechnology), 5 μM; RO31-8220 (Santa Cruz Biotechnology), 10 μM; Gö6983 (Santa Cruz Biotechnology), 10 μM; KT 5720 (Santa Cruz Biotechnology), 10 μM; U73122 (EMD Millipore), 1 μM. Phorbol-12-myristate-13-acetate (PMA, Sigma Aldrich) was used at a final concentration of 10 μM.
Ojani R., Liu P., Fu X, & Zhu J. (2015). Protein kinase C modulates transcriptional activation by the juvenile hormone receptor Methoprene-tolerant. Insect biochemistry and molecular biology, 70, 44-52.
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