One probe

Manufactured by Agilent Technologies

Sourced in United States

The One-probe is a versatile laboratory equipment designed for a range of applications. It provides a core function of measuring and analyzing various parameters with high precision and accuracy. The details of its intended use are not provided as part of this factual and unbiased description.

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Lab products found in correlation

500 mhz nmr spectrometer, by Agilent Technologies (2 mentions) Qstar xl hybrid lc ms ms system, by Thermo Fisher Scientific (2 mentions) Prominence hplc system, by Shimadzu (2 mentions) 400 mhz spectrometer, by Agilent Technologies (2 mentions) Nmr suite 8, by Chenomx (2 mentions) 2489 hplc system, by Waters Corporation (1 mentions) Synergy ht, by Agilent Technologies (1 mentions) Agilent 600 mhz spectrometer, by Agilent Technologies (1 mentions)

9 protocols using one probe

Analytical Techniques for Structural Elucidation

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UV–Vis spectra were obtained by using a Shimadzu Prominence HPLC System (Shimadzu, Kyoto, Japan) coupled with a PDA detector. High-resolution electrospray ionization mass spectra were gathered using a QSTAR® XL Hybrid LC/MS/MS System (Applied Biosystems, Foster City, CA, USA) equipped with turbo ion spray source, delivering the sample at a rate of 40 µl min⁻¹. NMR data were collected using a Varian 500-MHz NMR spectrometer with 5 mm Varian Oneprobe (¹H 500 MHz, ¹³C 125 MHz). Residual signals from solvents were used for referencing. Semipreparative HPLC was performed using a Phenomenex C18 column (Luna 250 × 10 mm, 5 µm, 4.0 ml min⁻¹). ECD spectra were obtained on an Aviv Biomedical Model 410 CD Spectrometer.

Miller B.W., Torres J.P., Tun J.O., Flores M.S., Forteza I., Rosenberg G., Haygood M.G., Schmidt E.W, & Concepcion G.P. (2020). Synergistic anti-methicillin-resistant Staphylococcus aureus (MRSA) activity and absolute stereochemistry of 7,8-dideoxygriseorhodin C. The Journal of Antibiotics, 73(5), 290-298.

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Quantitative NMR Analysis of AHA and 2,4-Diaminobutyric Acid

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¹H-NMR spectra were recorded on a Varian 400 MHz spectrometer with a broadband
auto-tune OneProbe (Varian). The spectra were recorded at 25°C without spinning (90°
excitation pulse, 2.6 s acquisition time) and continuous wave pre-saturation of the water
signal for 2 s was employed (4.6 s repetition rate). Sixty-four scans were recorded
each using identical parameters for all samples. Spectra were processed with inmr 4.1.7
software (Nucleomatica) using manual phase correction, 0.1 Hz of line broadening and
automatic baseline correction. The ratio of AHA to L-2,4-diaminobutyric acid was
quantified via integration of the signal RCH₂N₃ at
3.4 p.p.m. (AHA) and RCH₂NH₂ at 2.95 p.p.m.
(2,4-diaminobutyric acid). For incubations in the presence of sulfide, no decomposition products
other than 2,4-diaminobutyric acid could be detected. The identity of 2,4-diaminobutyric acid was
verified by reducing an aliquot of AHA with tris(2-carboxyethyl)phosphine (Staudinger reduction) and
comparing its NMR spectrum in pH 8.0 buffer with the sulfide incubation spectra.

Hatzenpichler R., Scheller S., Tavormina P.L., Babin B.M., Tirrell D.A, & Orphan V.J. (2014). In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry. Environmental Microbiology, 16(8), 2568-2590.

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Comprehensive Analytical Characterization of Compounds

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UV-vis spectra were obtained using a Shimadzu Prominence HPLC System (Shimadzu, Kyoto, Japan) coupled with a PDA detector. High-resolution electrospray ionization mass spectra were gathered using a QSTAR® XL Hybrid LC/MS/MS System (Applied Biosystems, Foster City, CA, USA) equipped with turbo ion spray source, delivering the sample at a rate of 40 μl/min. NMR data were collected using a Varian 500 MHz NMR spectrometer with 5 mm Varian Oneprobe (¹H 500 MHz, ¹³C 125 MHz). Residual signals from solvents were used for referencing. Semi-preparative HPLC was performed using a Phenomenex C18 column (Luna 250 X 10 mm, 5 μm, 4.0 ml/min). ECD spectra were obtained on an Aviv Biomedical Model 410 CD Spectrometer.

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Quantitative NMR Analysis of AHA and Diamino Acid

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¹H-NMR spectra were recorded on a Varian 400 MHz spectrometer with a broadband auto-tune OneProbe. The spectra were recorded at 25 °C without spinning (90° excitation pulse, 2.6 s acquisition time) and continuous wave pre-saturation of the water signal for 2 s was employed (4.6 s repetition rate). 64 scans were recorded each using identical parameters for all samples. Spectra were processed with iNMR 4.1.7 software using manual phase correction, 0.1 Hz line broadening and automatic baseline correction. The ratio of AHA to L-2,4-diaminobutyric acid was quantified via integration of the signal RCH₂N₃ at 3.4 ppm (AHA) and RCH₂NH₂ at 2.95 ppm (2,4-diaminobutyric acid). For incubations in the presence of sulfide, no decomposition products other than 2,4-diaminobutyric acid could be detected. The identity of 2,4- diaminobutyric acid was verified by reducing an aliquot of AHA with tris(2-carboxyethyl)phosphine (Staudinger reduction) and comparing its NMR spectrum in pH 8.0 buffer with the sulfide incubation spectra.

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Metabolite Profiling of Biosamples

Cited 3 times

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Prior to assay, samples were randomly assigned into batches to avoid potential batch effects. At the time of assay, samples were thawed in an ice water bath and subjected to a 1:1:1 sample/methanol/chloroform precipitation to remove macromolecules [26 (link)]. The resulting upper aqueous-methanol layers of each sample were dried by lyophilization (Labconco #7382021 at −105°C and 0.014mBar) and resuspended in 50mM sodium phosphate buffer in D₂O (500 μL) with DSS-d6 internal standard Chenomx IS-2 (Chenomx Inc., Edmonton, Alberta, Canada; chenomx.com) of known concentration. The 1-D-¹H-NMR spectrum of each WB sample was acquired at the University of Michigan’s Biochemical NMR Core Laboratory on a Varian (now Agilent, Inc., Santa Clara, CA) 11.74 Tesla (500 MHz) NMR spectrometer with a VNMRS console operated by host software VNMRJ4.0 and equipped with a 5-mm Agilent “One-probe” [27 (link)]. The resulting NMR spectra were analyzed using Chenomx NMR Suite 8.3 (Chenomx, Inc.) [28 (link)]. Spectral processing, compound identification, and quantification of metabolites were completed and reconciled by three users who were blinded to the samples’ identities.

Sun Y., Kim J.H., Vangipuram K., Hayes D.F., Lavoie Smith E.M., Yeomans L., Henry N.L., Stringer K.A, & Hertz D.L. (2018). Pharmacometabolomics Reveals a Role for Histidine, Phenylalanine and Threonine in the Development of Paclitaxel-induced Peripheral Neuropathy. Breast cancer research and treatment, 171(3), 657-666.

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Multi-Instrument Analytical Techniques

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(1) HPLC Waters HPLC system 2489, UV-Vis detector, 600 controller, and Empower Pro software. (2) LC-MS: Agilent LC-MS 6120B Quadrupole, 6550 A iFunnel Q-TOF. (3) NMR: Agilent NMR DD2 400 MHz with OneProbe (Agilent Technologies, Santa Clara, CA, USA). (4) Fluorescent plate reader (BIO-TEK/Synergy HT).

Ye Y., Toczek J., Gona K., Kim H.Y., Han J., Razavian M., Golestani R., Zhang J., Wu T.L., Ghosh M., Jung J.J, & Sadeghi M.M. (2018). Novel Arginine-containing Macrocyclic MMP Inhibitors: Synthesis, 99mTc-labeling, and Evaluation. Scientific Reports, 8, 11647.

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NMR Metabolomics Protocol for Biofluids

Cited 2 times

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Spectra for each whole blood and urine sample were acquired on a Varian (now Agilent, Inc., Santa Clara, CA) 11.74 Tesla (500 MHz) NMR spectrometer with a VNMRS console operated by host software VNMRJ 4.0, and equipped with a 5-mm Agilent “One-probe”. Thirty-two scans of the first increment of a 1 H,1 H-NOESY (commonly referred to as a 1D-NOESY or METNOESY) pulse sequence were used to create the NMR spectra as previously described.^{10 ,12 (link),14 (link)} All spectra were completed at room temperature (295.45 ± 0.3 K) at the University of Michigan’s Biochemical NMR Core Laboratory. The resulting spectra were analyzed using the Chenomx NMR Suite 8.0 (Chenomx, Inc.). The Processor Module of the software was utilized for phase shift and baseline correction, while the Profiler Module allowed for the identification and quantification of metabolites against an internal library of 312 compounds.

Feng Y., Wente S.R, & Majerus P.W. (2001). Overexpression of the inositol phosphatase SopB in human 293 cells stimulates cellular chloride influx and inhibits nuclear mRNA export. Proceedings of the National Academy of Sciences of the United States of America, 98(3), 875-879.

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Inert Atmosphere Synthesis of Pyridine Bismuth Complexes

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All syntheses were carried out on a vacuum line under a N2 atmosphere. Products were isolated and handled under a N2 atmosphere. Liquid pyridines, NMR solvents, and reaction solvents were stored over molecular sieves and degassed by three freeze-pump-thaw cycles under N2 prior to use. [Bi(6-Me-2-py)3] (1) was synthesized as described previously. NMR spectra were recorded on 500 MHz Agilent DD2 instruments equipped with a cold probe and a 400 MHz Agilent instrument equipped with a ONEPROBE in the Laboratory of Instrumental Techniques (LTI)
Research Facilities, University of Valladolid. Chemical shifts (δ) are reported in parts per million (ppm). 1 H and 13 C NMR are referenced to TMS. 7 Li and 19 F NMR experiments are referenced to a solution of LiCl/D2O and CCl3F, respectively. Coupling constants (J) are reported in Hz. was used as the matrix. Elemental analysis was obtained using a CHNS-932 Elemental Analyzer at the CAI of the University of Alcalá. In cases where we have been unable to obtain satisfactory elemental analysis, the formulations of the compounds are either supported by X-ray structure determination or high-resolution mass spectroscopy (in addition to NMR spectroscopy).

García-Romero Á., Plajer A.J., Miguel D., Wright D.S., Bond A.D., Álvarez C.M, & García-Rodríguez R. (2020). Tris(2-pyridyl) Bismuthines: Coordination Chemistry, Reactivity, and Anion-Triggered Pyridyl Coupling. Inorganic chemistry, 59(10).

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NMR Analysis of Bean Cotyledon

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For NMR analysis, the bean coat was manually separated from the cotyledon. The cotyledon were powdered, and approximately 30.0 mg were soaked in a mixture of 600 μL of D 2 O and 1 mM of TMSP-d 4 (sodium-3-trimethylsilylpropionate-2,2,3,3-d 4 ). The solutions were mixed for 2 min at room temperature and centrifuged at 804.6 g for 2 min. The supernatants were transferred to 5 mm NMR tubes. The NMR experiments were performed on an Agilent 600-MHz spectrometer equipped with a 5 mm (H-F/ 15 N-31 P) inverse detection One Probe™ with actively shielded Z-gradient. The 1 H NMR spectra were acquired with 32 free induction decays (FID), 48 k of time domain points for a spectral window of 16 ppm, under quantitative parameters at 298 K: acquisition time of 5.0 s and a recycling delay of 25.0 s (determined by the inversion-recovery pulse sequence). The spectra were processed by applying exponential line broadening of 0.3 Hz, zero filling of 32 k points, and referenced to the TMSP-d 4 resonance at δ 0.0. The identification of the constituents was performed through 1 H-1 H COSY, 1 H-13 C HSQC, and 1 H-13 C HMBC experiments, supplementary open access database (http://www.hmdb.ca) and literature reports (Alves Filho, Silva, Teofilo, Larsen, & de Brito, 2017; (link)Choze et al., 2013; (link)Lião et al., 2011) . Complete signaling is provided in Supporting Information (SI).

Coelho S.R.M., Filho E.G.A., Silva L., Bischoff T.Z., Ribeiro P.R.V., Zocolo G.J., Canuto K.M., Bassinello P.Z., & Brito E.S.d. (2020). NMR and LC-MS assessment of compound variability of common bean (Phaseolus vulgaris) stored under controlled atmosphere. LWT, 117, 108673-108673.

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