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Clone l3d10

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Clone L3D10 is a mouse monoclonal antibody that recognizes the CD3 epsilon chain, a component of the T cell receptor complex. This antibody can be used for the identification and enumeration of T cells in flow cytometry applications.

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Lab products found in correlation

Clone f38 2e2, by BioLegend (2 mentions)

Close spelling variants of this product

Anti-CTLA4 PE (clone L3D10,

2 protocols using clone l3d10

1

Modulating CD8+ T cell function

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dCD8+ T cells were cultured (5 × 105 per well) in the presence of anti-Tim-3 (10 μg/ml, clone F38-2E2, BioLegend, USA), anti-CTLA-4 (10 μg/ml, clone L3D10, BioLegend, USA), both of the two antibodies, or isotype control for 48 h. Brefeldin A, PMA, and ionomycin was added 4 h before the end of the culture. The cells were then collected for further analysis by flow cytometry.
Wang S., Sun F., Li M., Qian J., Chen C., Wang M., Zang X., Li D., Yu M, & Du M. (2019). The appropriate frequency and function of decidual Tim-3+CTLA-4+CD8+ T cells are important in maintaining normal pregnancy. Cell Death & Disease, 10(6), 407.
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2

Cytokine Production in CD4+ T Cells

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dCD4+T cells were cultured (5 × 105 per well) in the presence of anti-CTLA-4 (10 μg/ml, clone L3D10, BioLegend, U.S.A.), anti-Tim-3 (10 μg/ml, clone F38-2E2, BioLegend, U.S.A.), both of the two antibodies, or isotype control for 48 h. Brefeldin A, PMA, and ionomycin was added 4 h before the end of the culture. The cells were then collected for further analysis by flow cytometry.
Wang S., Chen C., Li M., Qian J., Sun F., Li Y., Yu M., Wang M., Zang X., Zhu R., Li D, & Du M. (2019). Blockade of CTLA-4 and Tim-3 pathways induces fetal loss with altered cytokine profiles by decidual CD4+T cells. Cell Death & Disease, 10(1), 15.
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