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4 protocols using alexa fluor 555 donkey anti mouse igg antibody a31570

1

Nix Protein Expression Analysis

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Anti-Nix antibody (12396S) was purchased from Cell Signaling Technology (United States). Anti-NeuN antibody-Neuronal cell marker (ab104224), anti-TOMM20 antibody (ab56783), and anti-β-tubulin antibody (ab179513) were purchased from Abcam (United States). Goat anti-rabbit IgG-HRP (sc-2004) and goat anti-mouse IgG-HRP (sc-2005) were purchased from Santa Cruz Biotechnology (United States). Alexa Fluor-488 donkey anti-rabbit IgG antibody (A21206) and Alexa Fluor-555 donkey anti-mouse IgG antibody (A31570) were purchased from Invitrogen (United States). Nix overexpression plasmid and siRNA were purchased from GenScript (China).
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2

Curcumin-induced Autophagy and Angiogenesis

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Cur was extracted from Curcuma longa (Bailingwei Technology Co., Ltd., Beijing, China, SB-431542). The rabbit polyclonal antibody against LC3-A/B (ab58610) and the mouse monoclonal antibody against p62 (610833) were purchased from BD Biosciences (Palo Alto, CA, USA) and the mouse monoclonal antibody against GAPDH (sc-365062) was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The FOX3-vWF rabbit polyclonal and mouse monoclonal antibody-endothelial cell marker (ab6994 and ab68545) were from Abcam (New Territories, Shatin, Hong Kong). Secondary antibodies for western blot analysis, which included goat anti-rabbit IgG-HRP (sc-2004) and goat anti-mouse IgG-HRP (sc-2005) were purchased from Santa Cruz Biotechnology, Inc.. Normal rabbit IgG (sc-2027) was from Santa Cruz Biotechnology, Inc. Secondary antibodies for immunofluorescence, including Alexa Fluor-488 donkey anti-rabbit IgG antibody (A21206) and Alexa Fluor-555 donkey anti-mouse IgG antibody (A31570) were from Invitrogen-Life Technologies (Carlsbad, CA, USA).
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3

Antibody Selection for Neurological Studies

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Rabbit anti-GPR18 polyclonal antibody (SAB4501252) was provided by Sigma. Rabbit anti-albumin polyclonal antibody (ab51422), rabbit anti-eNOS polyclonal antibody (ab66127), rabbit anti-nNOS monoclonal antibody (ab76067), rabbit anti-5-LOX monoclonal antibody (ab169755), mouse anti-NeuN antibody (ab104224), mouse anti-GFAP antibody (ab10062), and mouse anti-von Willebrand factor (vWF) antibody (ab68545) were purchased from Abcam (Cambridge, MA, USA). Rabbit anti-5-LOX (phospho-Ser663) polyclonal antibody (P12527) was purchased from Biorbyt (Cambridge, CA, UK). Rabbit anti-ERK1/2 (phospho-Thr202/Tyr204) antibody (#4370) and monoclonal rabbit anti-β-tubulin antibody (#2146) were provided by Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-ZO-1 polyclonal antibody (RA231621) was purchased from Thermo Fisher (Waltham, MA, USA). Goat anti-rabbit IgG HRP (sc-2004) was purchased from Santa Cruz Biotechnology (CA, USA). Alexa Fluor-488 donkey anti-rabbit IgG antibody (A21206) and Alexa Fluor-555 donkey anti-mouse IgG antibody (A31570) were purchased from Invitrogen (Carlsbad, CA, USA).
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4

Antibodies for Cellular Protein Analysis

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Antibodies against cleaved caspase-3 (9661), LC3 (2775), and Atg7 (2631) were purchased from Cell Signaling Technology. Sec22b antibody (14776-1-AP) was purchased from Proteintech. Ykt6 antibody (ab236583), NeuN antibody (ab104224), and GFAP antibody (ab134436) were from Abcam. CD31 antibody (AF3628) was purchased from R&D Systems. Antibodies against β-tubulin (sc-5274), normal rabbit IgG (sc-2027), and horseradish peroxidase-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology. Alexa Fluor-488 donkey anti-rabbit IgG antibody (A21206), Alexa Fluor-555 donkey anti-mouse IgG antibody (A31570), and Alexa Fluor-555 donkey anti-goat IgG antibody (A21432) were purchased from Invitrogen.
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