Innoscanw 710 al

TE cells were lysed, and genomic DNA was amplified using the SurePlex DNA Amplification System (BlueGnome, Cambridge, UK), according to the manufacturer's instructions. Whole Genome Amplification (WGA) products were processed as reported elsewhere [17 (link)] according to the BlueGnome 24sure V3 protocol (available at http://www.cytochip.com/). Briefly, WGA products were fluorescently labelled and competitively hybridized to 24sure V3 arrays (BlueGnome, Cambridge, UK) with a matched control in an array CGH experiment format. A laser scanner InnoScanw 710 AL (INNOPSYS, Carbonne, France) was used to excite the hybridized fluorophores and read and store the resulting images of the hybridization. Scanned images were then analysed and quantified by algorithm fixed settings in BlueFuse Multi Software (BlueGnome, Cambridge, UK), a software package that performed the steps of grid placement, quantification, normalization, and postprocessing automatically. The whole procedure was completed within 12–24 h, and the results were obtained in time for an embryo transfer at day 6, in a fresh cycle.

Genomic DNA samples were fluorescently labelled and competitively hybridized to CytoChip Focus Constitutional microarrays (Illumina, USA) with a normal male control gDNA in an array CGH experiment format. A laser scanner InnoScan w710AL (Innopsys, France) was used to excite the hybridized fluorophores and read and store the resulting images of the hybridization. Scanned images were then analyzed and quantified by an algorithm with fixed settings in BlueFuse Multi Software (Illumina, USA) (available protocol at www.cytochip.com).

The hBES-1 (P42), hPES-1 (P70), hPES-2 (P20) and hPES-2 (P55) cell colonies were lysed, after which genomic DNA was amplified using a SurePlex DNA Amplification system (BlueGnome Ltd., Cambridge, UK), according to the manufacturer’s instructions. Whole genome amplification (WGA) products were processed as previously described (24 (link)) according to the BlueGnome protocol (available at: http://www.cytochip.com/). Briefly, WGA products were fluorescently labeled and competitively hybridized to 24sure V3/24sure + arrays with matched control SureRef reference DNA (male/female) (both from BlueGnome Ltd.) in an array CGH experiment format. A laser scanner, InnoScanw 710 AL (Innopsys, Carbonne, France), was used to excite the hybridized fluorophores of a Fluorescent Labelling system (BlueGnome Ltd.), and used to read and store the resulting hybridization images. The scanned images were then analyzed and quantified using algorithm fixed settings with BlueFuse Multi software (BlueGnome Ltd.), a software package that automatically performed the steps of grid placement, quantification, normalization and post-processing.