C57bl 6

Manufactured by Jackson ImmunoResearch

Sourced in United States, Montenegro, United Kingdom, Germany, Australia, China, Canada

C57BL/6 is a widely used inbred mouse strain. It is a robust, readily available laboratory mouse model.

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Lab products found in correlation

2 462 protocols using c57bl 6

Transgenic Mouse Strains for Regenerative Medicine

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The following strains of mice were purchased from Jackson Laboratories and used in this study: wild type C57BL/6 (Cat# 000664), transgenic EGFP C57BL/6 (Cat# 004353), mixed 129S C57BL/6 knock-in mice expressing EGFP from the Sox2 promoter (Sox2^EGFP) (Cat# 017592), mixed 129S C57BL/6 knock-in mice expressing tamoxifen inducible Cre from the Sox2 promoter (Sox2^CreERT2) (Cat# 017593), C57BL/6 transgenic loxP-stop-loxP EYFP (Cat# 006148), and mixed 129S C57BL/6 floxed Sox2 mice (Sox2^flox) (Cat# 013093). Mice were bred and housed in the Division of Laboratory Animal Resources facility at the University of Pittsburgh McGowan Institute for Regenerative Medicine. Experimental protocols followed US National Institute of Health guidelines for animal care and were approved by the Institutional Animal Care and Use Committee at the University of Pittsburgh.

DeWard A.D., Cramer J, & Lagasse E. (2014). Cellular heterogeneity in the mouse esophagus implicates the presence of a non-quiescent epithelial stem cell population. Cell reports, 9(2), 701-711.

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Transgenic Mouse Strains for Immunological Research

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C57BL/6.FoxP3^GFP, C57BL/6.FoxP3^GFPIL-10 ^Thy1.1, OTII.FoxP3^GFP and 2D2.FoxP3^GFP mice were bred and housed in our animal facility. C57BL/6, C57BL/6.CD45.1, C57BL/6.IL-6^−/−, C57BL/6, Tg(Cd4-cre)1Cwi (CD4^Cre) and B6.129S1-Stat3tm1Xyfu/J (STAT3^flox/flox) mice were bought from Jackson and the latter were interbred in our animal facility to obtain CD4^CreSTAT3^flox/flox mice. 129S6/SvEv-Stat1^tm1Rds (STAT1^−/−) and 129S6/SvEvTac (129S6) control mice were purchased from Taconics. All experiments were performed under specific pathogen-free conditions in our animal facility at the Harvard Institutes of Medicine and according to the animal protocol guidelines of the Committee on Animals of Harvard Medical School, which also approved the experiments.

Kuhn C., Rezende R.M., M'Hamdi H., da Cunha A.P, & Weiner H.L. (2016). IL-6 inhibits upregulation of membrane-bound TGF-beta 1 on CD4+ T cells and blocking IL-6 enhances oral tolerance. Journal of immunology (Baltimore, Md. : 1950), 198(3), 1202-1209.

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Mouse Model Comparison for Immune Studies

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BALB/c (catalog no. 000651), CBA/J (catalog no. 000656), C57BL/6 (catalog no. 000664), Card9^−/− (catalog no. 028652), and MyD88^−/− (catalog no. 009088) mice were obtained from Jackson Laboratory (Bar Harbor, ME). BALB/c and C57BL/6 mice obtained from Jackson Laboratory are also known as BALB/cJ and C57BL/6 mice, respectively. All mice were 6 to 8 weeks old at the time of inoculation. All animal protocols were reviewed and approved by the Animal Studies Committee of the Washington University School of Medicine and conducted according to National Institutes of Health guidelines for housing and care of laboratory animals.

Hole C.R., Lam W.C., Upadhya R, & Lodge J.K. (2020). Cryptococcus neoformans Chitin Synthase 3 Plays a Critical Role in Dampening Host Inflammatory Responses. mBio, 11(1), e03373-19.

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Mice Strains for Airway Experiments

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BALB/c, C57BL/6, BALB/cByJ, Tlr4^Lp^s^-d/J (Tlr4-d, BALB/cByJ background), Rag1^−/− mice (BALB/c background), Il1r1^−/− mice (C57BL/6 background), and PAR2^−/− mice (C57BL/6 background) were purchased from the Jackson Laboratory (Bar Harbor, ME). ST2^−/− (Il1rl1^−/−) mice (BALB/c background), Il17rb^−/− mice (BALB/c background) and Il13^+/eGFP mice (BALB/c background) were provided by Dr. Andrew McKenzie (Medical Research Council Laboratory of Molecular Biology, Cambridge, UK). Tslpr^−/− mice (BALB/c background) were provided by Dr. Steven Ziegler (Benaroya Institute, Seattle, WA). Il5^+/venus mice (BALB/c background) were provided by Dr. Kiyoshi Takatsu (University of Toyama, Toyama, Japan). Nlrp3^−/− mice (C57BL/6 background) were provided by Dr. Jurg Tschopp (University of Lausanne, Switzerland). All knockout or transgenic mice were bred in the Mayo Clinic animal care facility, and female mice aged 6–10 weeks were used for studies. All animal experiments and handling procedures were approved by the Mayo Clinic Institutional Animal Care and Use Committee and performed according to their guidelines.
Normal human bronchial airway epithelial (NHBE) cells were purchased from Lonza (Allendale, NJ) and maintained in serum-free bronchial epithelial cell growth medium (Lonza). NHBE cells were used within three passages.

Hara K., Iijima K., Elias M.K., Seno S., Tojima I., Kobayashi T., Kephart G.M., Kurabayashi M, & Kita H. (2014). Airway uric acid is a sensor of inhaled protease allergens and initiates type 2 immune responses in respiratory mucosa. Journal of immunology (Baltimore, Md. : 1950), 192(9), 4032-4042.

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Hearing Loss Comparison in Mice

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CBA/CaJ, C57BL/6 and B6.cdh23^+/− mice were either obtained from The Jackson Laboratory (stocks #000664 and #002756) or the offspring from the Jax mice, and the F1(CBA/CaJ x C57BL/6) are the first generation offspring from the CBA/CaJ and C57BL/6 cross. For each strain, we used two- to four-month mice of each sex.

Si Y., Ito S., Litke A.M, & Feldheim D.A. (2022). High-Frequency Hearing Is Required to Compute a Topographic Map of Auditory Space in the Mouse Superior Colliculus. eNeuro, 9(3), ENEURO.0513-21.2022.

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Mouse Breeding and Euthanasia Procedures

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C57BL/6, OT I (C57BL/6-Tg(TcraTcrb)1100Mjb/J), and OT II (C57BL/6-Tg(TcraTcrb)425Cbn/Crl)
mice were purchased from Jackson Laboratory (Bar Harbor, ME) and bred
at the University of Leiden (The Netherlands). The mice were kept
under standard laboratory conditions, with food and water provided ad libitum. The mice were euthanized while sedated, by cervical
dislocation. All animal work was performed according to the guidelines
of the European Parliament Directive 2010/63EU, and the experimental
work was approved by the Animal Ethics Committee of Leiden University.
For culture conditions of BMDCs, see below.

Guzelj S., Weiss M., Slütter B., Frkanec R, & Jakopin Ž. (2022). Covalently Conjugated NOD2/TLR7 Agonists Are Potent and Versatile Immune Potentiators. Journal of Medicinal Chemistry, 65(22), 15085-15101.

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Adoptive Transfer of OT-I Transgenic T Cells

Cited 1 time

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C57BL/6 mice were from Charles River/NCI. Nur77-GFP reporter (C57BL/6-Tg(Nr4a1-EGFP/cre)820^Khog/J) (37 (link)), OT-I transgenic (C57BL/6-Tg(TcraTcrb)1100^Mjb/J) (38 (link)), Thy1.1 congenic mice (B6.PL-Thy1^a/CyJ) (39 (link)), Rag1^ko mice (B6.Rag1^em10Lutzy) and Perforin^ko (C57BL/6-Prf1tm1Sdz/J) (40 (link)) mice, all from Jackson Laboratories, were bred and maintained in a pathogen-free facility at the University of Virginia. Six to 12-week-old Nur77-GFP x (OT-I x Thy1.1) F1 mice were the source of mice expressing OT-I⁺Thy1.1⁺Nur77-GFP⁺ cells used for adoptive transfer. All procedures were approved by the University of Virginia Animal Care and Use Committee in accordance with the NIH Guide for Care and Use of Laboratory Animals.

Lindsay R.S., Melssen M.M., Stasiak K., Annis J.L., Woods A.N., Rodriguez A.B., Brown M.G, & Engelhard V.H. (2022). NK cells reduce anergic T cell development in early-stage tumors by promoting myeloid cell maturation. Frontiers in Oncology, 12, 1058894.

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Generation and Characterization of Conditional Cbfβ Knockout Mice

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C57BL/6 were purchased from Jackson Laboratory (Bar Harbor, ME). Rec-Vα14Tg TCR transgenic mice were generated in Dr. Derek Sant’Angelo laboratory^{9 (link)}, which require Rag-mediated recombination to produce a functional TCR (Supplementary Fig. S1a). These mice have increased numbers of iNKT cells, as compared with C57BL/6 mouse. Mice carrying a conditional floxed allele of Cbfβ (Cbfβ^fl/fl) were previously described^{45 (link)} and provided by Dan R. Littman (New York University, New York, NK). Mice were backcrossed to the C57BL/6 background for 7 generations and then mated to C57BL/6 mice carrying the Cd4 enhancer/promoter/silence Cre allele (obtained from The Jackson Laboratory), to generate CD4^CreCbfβ^fl/fl conditional knockout mice (Cbfβ KO). The full list of mouse strains used can be found in Supplementary Table S1. 5-week-old, sex-matched mice were utilized in this study. All studies, protocol, and mouse handling were approved by the Institutional Animal Care and Use Committee.

Wang J., Adrianto I., Subedi K., Liu T., Wu X., Yi Q., Loveless I., Yin C., Datta I., Sant’Angelo D.B., Kronenberg M., Zhou L, & Mi Q.S. (2023). Integrative scATAC-seq and scRNA-seq analyses map thymic iNKT cell development and identify Cbfβ for its commitment. Cell Discovery, 9, 61.

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Dysferlin-null Mice and Controls

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Dysferlin-null (A/J) and control (A/JCr, C57Bl/6) mice were obtained from the Jackson Laboratory (A/J, C57Bl/6) or the National Cancer Institute, Frederick, MD (A/JCr) or bred at the University of Maryland, Baltimore (C57Bl/6). Mice were anesthetized with 2.5–4.5% isoflurane vaporized in oxygen and euthanized by cervical dislocation. Mice were 12–16 weeks of age at the time their tissues were studied.

Lukyanenko V., Muriel J., Garman D., Breydo L, & Bloch R.J. (2022). Elevated Ca2+ at the triad junction underlies dysregulation of Ca2+ signaling in dysferlin-null skeletal muscle. Frontiers in Physiology, 13, 1032447.

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Myeloid-specific Sirt1 Deficiency Mice

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C57BL/6 (Orient Bio) and Nlrp3^-/- (Jackson Laboratory) mice were bred at Yonsei University College of Medicine under specific pathogen-free conditions. To obtain myeloid-specific Sirt1-deficient mice (Sirt1^fl/fl;LysM Cre mice), homozygous Sirt1^fl/fl mice (C57BL/6) were crossed with LysM Cre transgenic mice (C57BL/6, Jackson laboratory). Mice aged 9–12 weeks were used in the experiments. All experimental procedures were approved by the Institutional Ethical Committee, Yonsei University College of Medicine. Animal experiments were performed in accordance with the guidelines of the Institutional Ethical Committee. Mice were shaved 24 h prior to injection, and intradermally administered with FK866 (7 mg/kg) once a day, for two consecutive days. After the last FK866 injection, ATP was intradermally administered (12.5 mg/kg) at the same injection site. Six hours after ATP injection, the mice were sacrificed and subjected to various analyses.

Shim D.W., Cho H.J., Hwang I., Jung T.Y., Kim H.S., Ryu J.H, & Yu J.W. (2021). Intracellular NAD+ Depletion Confers a Priming Signal for NLRP3 Inflammasome Activation. Frontiers in Immunology, 12, 765477.

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