Am 400 spectrometer

Manufactured by Bruker

Sourced in Germany, United States, Switzerland

The AM-400 spectrometer is a laboratory equipment designed for nuclear magnetic resonance (NMR) spectroscopy. It is capable of performing high-resolution NMR analysis on a variety of samples, providing detailed information about the chemical structure and composition of the analyzed materials.

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Lab products found in correlation

Silica gel, by Qingdao Haiyang Chemical (16 mentions) Lct premier xe spectrometer, by Waters Corporation (6 mentions) Lc ltq orbitrap xl spectrometer, by Thermo Fisher Scientific (6 mentions) Sephadex lh 20 gel, by Cytiva (5 mentions) Vertex 70, by Bruker (5 mentions) Sephadex lh 20, by GE Healthcare (5 mentions) Silica gel, by Qingdao Marine Chemical (5 mentions) 341 polarimeter, by PerkinElmer (5 mentions) Nexus 670 ft ir spectrophotometer, by Thermo Fisher Scientific (5 mentions) Lc 20at, by Shimadzu (4 mentions) Rp c18 f254 plates, by Merck Group (4 mentions) Micromass q tof spectrometer, by Waters Corporation (3 mentions) Cary 50, by Jasco (3 mentions) Xb c18, by Hill-Rom (3 mentions) Mci gel chp 20p, by Mitsubishi (3 mentions) Hplc system, by Thermo Fisher Scientific (3 mentions) Cary 500 spectrophotometer, by Agilent Technologies (3 mentions) Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (3 mentions) Nicolet 6700, by Thermo Fisher Scientific (3 mentions) Pre coated silica gel gf254 plates, by Qingdao Haiyang Chemical (3 mentions)

101 protocols using am 400 spectrometer

Characterization of Fe-based Compound

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Anhydrous solvent pyridine, CH₃CN, CH₂Cl₂, and N,N-Dimethylformamide (DMF) were distilled under a nitrogen atmosphere and stored with 4 Å molecular sieves. Proton NMR spectra were recorded on a Bruker AM400 spectrometer. ¹H-NMR, ¹³C-NMR, and ³¹P-NMR spectra were recorded on a Bruker AM400 spectrometer. Chemical shifts (δ) are reported in ppm, and coupling constants (J) are in hertz (Hz). The following abbreviations were used to explain the multiplicities: s singlet, d doublet, t triplet, q quartet, m multiplet, br broad.
Fourier transform infrared spectroscopy (FTIR) samples were prepared by mixing Fe-base with pre-dried KBr powder. FTIR spectra were recorded on an IR spectrometer instrument (Thermo, Nicolet 6700) in the 4000–400 cm⁻¹ range.
The precise molecular weight of Fe-base was confirmed by a high-performance liquid chromatography-ion trap time-of-flight mass spectrometer (LCMS-IT-TOF, Shimadzu, Kyoto, Japan) equipped with an electrospray ionization (ESI) source, operating in positive ionization mode. The scanning ranges were m/z 50–1000. Shimadzu’s LCMS Solution software was used for data analysis.

Tan J., Li H., Ji C., Zhang L., Zhao C., Tang L., Zhang C., Sun Z., Tan W, & Yuan Q. (2022). Electron transfer-triggered imaging of EGFR signaling activity. Nature Communications, 13, 594.

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Synthesis and Purification of Fluorescent Nucleic Acid Probes

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All DNA synthesis reagents were purchased from Glen Research. FNA F6, F4, FM6 and M6 were synthesized and purified by Sangon Biotech (Shanghai). FNA Ft6, Ft8 and other oligonucleotides were synthesized on an ABI 3400 synthesizer (Applied Biosystems). Dabcyl CPG was used for all FAM-labeled FNA. The completed sequences were then deprotected in AMA (ammonium hydroxide/40% aqueous methylamine, 1:1) at 65 °C for 30 min and further purified by reversed-phase HPLC (ProStar; Varian) on a C-18 column using 0.1 M triethylamine acetate(TEAA) buffer (Glen Research) and acetonitrile (SigmaAldrich) as the eluents. The collected DNA products were dried and detritylated by dissolving and incubating DNA products in 200 μL of 80% acetic acid for 20 min. The detritylated DNA product was precipitated with NaCl (3 M, 25 μL) and ethanol (600 μL).
Unless otherwise noted below, all commercially available reagents and solvents were purchased from Sigma Aldrich and used without further purification. ¹H NMR (TMS as the internal standard) and ¹⁹F NMR spectra (CFCl₃ as the outside standard and low field positive) were recorded on a Bruker AM300 or Bruker AM400 spectrometer. ¹³C NMR was recorded on a Bruker AM400 spectrometer. Chemical shifts (δ) are reported in ppm, and coupling constants (J) are in Hertz (Hz).

Wang R., Wang C., Cao Y., Zhu Z., Yang C., Chen J., Qing F.L, & Tan W. (2014). Trifluoromethylated Nucleic Acid Analogues Capable of Self-Assembly through Hydrophobic Interactions. Chemical science (Royal Society of Chemistry : 2010), 5(10), 4076-4081.

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Analytical Characterization Protocols for Organic Compounds

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All reagents were of analytic grade and obtained from commercial suppliers and used without further purification. Reactions were monitored by GC-MS or thin-layer chromatography (TLC) on silica gel 60 GF₂₅₄ with ultraviolet detection. Melting points were measured in an open capillary using Büchi melting point B-540 apparatus and are uncorrected. ¹H and ¹³C NMR spectra were recorded on a Bruker AM-400 spectrometer (400 MHz and 100 MHz, respectively) using TMS as the internal standard. CDCl₃ and DMSO-d₆ were used as NMR solvent. The ¹⁹F NMR spectra were recorded on a Bruker AM-400 spectrometer (376 MHz) using CF₃CO₂H as external standard. Gas chromatography-mass spectra (GC-MS) were recorded on HP 5973 MSD with 6890 GC. High resolution mass spectra (HRMS) were recorded under electron impact conditions using a MicroMass GCT CA 055 instrument.

Zhao X., Xu S., Liu C., He J., Li C., Deng Y, & Cao S. (2020). Design, synthesis and insecticidal activity of novel analogues of flubendiamide containing alkoxyhexafluoroisopropyl groups. RSC Advances, 10(57), 34486-34492.

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Preparative HPLC Purification and Characterization

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The fractions were further purified by a preparative HPLC apparatus (PHPLC, Agilent 1200s, Agilent Technologies Inc., Palo Alto, CA, United States) equipped with a G13611A prep pump, G2260A prep automatic sampler, G1315D diode array detector, G1364B prep fraction collector and Agilent Zorbax SB-C₁₈ column (21.2 mm × 250 mm, 7 μm). The elution was carried out in isocratic mode with different ratios of methanol and water containing 0.1% formic acid at 5 mL/min. The detection wavelengths were 210, 250, and 280 nm. The PHPLC fractions were collected and evaporated to dryness.
The target compounds obtained by HSCCC and PHPLC separation were identified by their UV, MS,¹ H-NMR and ¹³C-NMR spectra. The NMR spectra were recorded on Bruker AM-400 spectrometers (Bruker, Karlsruhe, Germany) using trimethylsilyl (TMS) as the internal reference.

Ning Z., Wang C., Liu Y., Song Z., Ma X., Liang D., Liu Z, & Lu A. (2018). Integrating Strategies of Herbal Metabolomics, Network Pharmacology, and Experiment Validation to Investigate Frankincense Processing Effects. Frontiers in Pharmacology, 9, 1482.

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Comprehensive Characterization of Natural Products

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The IR data were obtained on a Tensor27 FT-IR spectrometer (Bruker, Billerica, MA, USA). The UV spectra were measured on a UV-2401A spectrophotometer (Shimadzu Corp., Kyoto, Japan). The optical rotation was determined on a SEPA 300 polarimeter (Horiba, Kyoto, Japan). 1D and 2D-NMR spectra were recorded on Bruker DRX-500 and AM-400 spectrometers with tetramethylsilane as an internal standard. CD data were obtained using a Chirascan CD spectrometer (Applied Photophysics, Surrey, UK). The HR-ESI-MS data were obtained on a 6200 Q-TOF MS system (Agilent Technologies, Santa Clara, CA, USA). Silica gel (200–300 mesh, Qingdao Haiyang Chemical Co., Ltd., Qingdao, China) and Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden) were used for column chromatography (CC). Preparative HPLC was performed on an Agilent 1260 instrument with an Agilent Zorbax SB-C18 column (5 lm, 9.4 × 150 mm). Fractions were monitored by TLC, and spots were visualized by spraying with 10% H₂SO₄ in EtOH, followed by heating.

Wang G.K., Jin W.F., Zhang N., Wang G., Cheng Y.Y., Morris-Natschke S.L., Goto M., Zhou Z.Y., Liu J.S, & Lee K.H. (2019). Kalshiolin A, new lignan from Kalimeris shimadai. Journal of Asian natural products research, 22(5), 489-495.

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Photochemical Synthesis and Biological Evaluation

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Chemicals were used as received unless otherwise indicated. All oxygen or moisture-sensitive reactions were performed under argon atmosphere using the standard Schlenk method. All other reagents are of analytical purity and used without further purification. Solvents used are of analytical grade, except those for recrystallization and optical tests, which were distilled prior to use. Thin-layer chromatography (TLC) was carried out on aluminum sheets coated with silica gel 60 F254 (MERCK).¹H NMR and¹³C NMR spectra were recorded using Bruker AM-400 spectrometers. DMSO-d₆, CDCl₃ and D₂O were used as solvent. Absorption and fluorescence spectra were recorded using Varian Cary 500 and Varian Cary Eclipse, respectively. The UV (365 nm, 2.6 mW cm⁻²) and light-emitting diode (LED) lamps M530L2 (Thorlabs; Norminal Wavelength 530 nm, Bandwidth (FWHM) 33 nm, 150 mW) were used as light sources for UV and visible light irradiation, respectively. Hep-G2 (HB-8065^TM), HeLa (CCL-2^TM) and A549 (CCL-185^TM) were obtained from ATCC (American Type Culture Collection).

Zhang J., Fu Y., Han H.H., Zang Y., Li J., He X.P., Feringa B.L, & Tian H. (2017). Remote light-controlled intracellular target recognition by photochromic fluorescent glycoprobes. Nature Communications, 8, 987.

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Rewritable Filter Paper Encoding

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All starting chemicals were commercially available and analytical purity without further treatment. Solvents were distilled and dried or degassed if necessary before use. Thin-layer chromatography (TLC) analysis was performed on silica gel plates and column chromatography was conducted using silica gel column packages purchased from Yantai HuangHai Chemical (China). NMR spectra were recorded on Bruker AM-400 spectrometers with tetramethylsilane as an internal reference, CDCl₃ as the solvent. High resolution mass (HRMS) spectra were measured on a Waters LCT Premier XE spectrometer. For rewritable application on filter papers, a portable PLS-LED light (Beijing perfectlight technology co., LTD, 420 nm, 30 W) and a commercial flashlight (5 W) with the interference filter (>550 nm) were used and the distances between the light sources and the filter paper were 20 and 2 cm, respectively. Except where noted, all tests were carried out at room temperature.

Zhang Z., Wang W., Jin P., Xue J., Sun L., Huang J., Zhang J, & Tian H. (2019). A building-block design for enhanced visible-light switching of diarylethenes. Nature Communications, 10, 4232.

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NMR and HRESIMS Analysis Protocol

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1D (¹H, ¹³C, and DEPT) and 2D (COSY, HMQC, and HMBC) NMR spectra were obtained using Bruker AM 400 spectrometers (Bruker, USA) with tetramethylsilane (TMS) as the internal standard. HRESIMS (high-resolution electrospray ionization mass spectrometry) analysis was conducted on an ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometer (UPLC-QTOF-MS, Waters, USA) in the positive-ion mode.

Lee J., Chun H.W., Pham T.H., Yoon J.H., Lee J., Choi M.K., Ryu H.W., Oh S.R., Oh J, & Yoon D.Y. (2019). Kanakugiol, a Compound Isolated from Lindera erythrocarpa, Promotes Cell Death by Inducing Mitotic Catastrophe after Cell Cycle Arrest. Journal of Microbiology and Biotechnology, 30(2), 279-286.

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Photochromic Compound Characterization

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NMR spectra were recorded using Bruker AM-400 spectrometers. CDCl₃ were used as solvents with tetramethylsilane (TMS) as an internal reference. High resolution mass spectra (HRMS) were carried our with a Waters LCT Premier XE spectrometer. HPLC analyses were performed by using an Agilent 1100 instrument equipped with CHIRALCEL® OD-R column (4.6 diameter × 250 mm), at flow rate of 0.8 mL min⁻¹, eluent solvents of CH₃CN/H₂O (80/20, v/v), detecting at isobestic point wavelength of 303 nm. Absorption and CD spectra were recorded using Agilent Cary 60 and Jasco J-819 spectropolarimeter, respectively. Optical rotation values were determined with a Rudolph Autopol V polarimeter, equipped with an interference filter at 633 nm, containing a 100 mm flow cell at 293 K. Solvents used were analytical grade, except those for optical tests, which were HPLC grade. The photochromic reaction was induced by continuous irradiation using an Hg/Xe lamp (Hamamatsu, LC8 Lightningcure, 200 W) or a white LED (3 W) equipped with a narrow band interference filters for λ_irr = 280 nm, or a broad band interference filters for λ_irr > 470 nm, except for the photocyclization in the PDLLA film which was induced by a hand-held UV lamp (0.12 mW cm⁻², λ = 302 ± 20 nm) for irradiation.

Li W., Li X., Xie Y., Wu Y., Li M., Wu X.Y., Zhu W.H, & Tian H. (2015). Enantiospecific photoresponse of sterically hindered diarylethenes for chiroptical switches and photomemories. Scientific Reports, 5, 9186.

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Analytical Methods for Compound Characterization

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Optical rotations were measured on a Rudolph Autopol I automatic polarimeter, UV spectra on a Shimadzu UV-2450 spectrophotometer, and IR spectra on Bruker Tensor 37 infrared spectrophotometers. NMR spectra were measured on Bruker AM-400 spectrometers at 25 °C. ESIMS was measured on a Finnigan LCQ Deca instrument, and HRESIMS was performed on a Waters Micromass Q-TOF spectrometer. Silica gel (300–400 mesh, Qingdao Haiyang Chemical Co., Ltd.), reversed-phase C₁₈ (RP-C₁₈) (12 nm, S-50 μm, YMC Co., Ltd.), and Sephadex LH-20 gel (Amersham Biosciences) were used for column chromatography (CC). A Shimadzu LC-20 AT equipped with a SPD-M20A PDA detector was used for HPLC. A YMC-pack ODS-A column (250 × 10 mm, S-5 μm, 12 nm) and a Phenomenex Lux cellulose-2 chiral column (10 × 250 mm, 5 μm) were used for semi-preparative HPLC separation. All of the solvents used were of analytical grade (Guangzhou Chemical Reagents Company, Ltd.).

Chen L., Liu B., Deng J.J., Zhang J.S., Li W., Ahmed A., Yin S, & Tang G.H. (2018). Lindera cyclopentenedione intermediates from the roots of Lindera aggregata. RSC Advances, 8(32), 17898-17904.

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