The largest database of trusted experimental protocols

180 protocols using jsm 6360lv

1

Scanning Electron Microscopy and Shear Bond Strength of Dental Resin-Cement Interface

Check if the same lab product or an alternative is used in the 5 most similar protocols
One specimen from each group was randomly selected and coated with gold before scanning electron microscope (SEM) (JSM-6360LV, JEOL, Kyoto, Japan) examination. The acceleration voltage of SEM was set to 20 kV, and the images were taken at magnifications of 1,000 times.
Specimens are collected using a slow cutting machine (M618, Jiangsu Nantong machine tool factory, China), applied perpendicular to the bonding interface between the resin and cement. Then, 400# and 800# sand paper was used for polishing, and after polishing, the specimens were cleaned in distilled water by ultrasonic cleaning for 5 minutes, followed by drying and sputtering with gold. Finally, the specimens were evaluated with a SEM (JSM-6360LV, JEOL ltd., Kyoto, Japan).
The specimens were then fixed to a specially fabricated jig and mounted to a universal testing machine (MTS-585, MTS, USA), and a load was applied at a crosshead speed of 0.5 mm/min until failure. The maximum force at debonding was recorded, and the shear bond strength (SBS) was calculated.
After SBS testing, the fractured interface was examined using stereomicroscopy (magnification ×30; KH7700, Hirox, Japan). The specimens were classified according to fracture patterns: type I: adhesive failure, type II: mixed failure (combination of cohesive and adhesive failure), and type III: cohesive failure.
+ Open protocol
+ Expand
2

Scanning Electron Microscope Analysis of Composites

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three specimens from each composite and F/P system were selected (highest, median, and lowest value of Ra), and scanning electron microscope (SEM) images were obtained. Each specimen was analyzed qualitatively using SEM (JEOL JSM-6360LV, JEOL, Tokyo, Japan) after sputter coating with gold (JEOL ION SPUTTER JFC-1100, JEOL, Tokyo, Japan) for 5 min with 10 mA current with an accelerating voltage of 15 kV. The surfaces were examined at × 50 to × 500 and representative photomicrographs were taken for each sample.
+ Open protocol
+ Expand
3

Nanoparticle Size Characterization of Hesperidin Nanoemulsions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Utilizing the microscope SEM JEOL JSM-6360 LV (JEOL, Akishima, Tokyo, Japan) and an atomic force microscope (Shimadzu, SPM 9500J3), the size of the nanoparticles in the nanoemulsions was verified. The sonicated hesperidin nanoemulsion was dropped onto a sample holder, dried at room temperature, and coated with gold or platinum using a MED 020 Sputter (BalTech, Balzers, Liechtenstein). The images were obtained using an acceleration voltage of 10 kV and a secondary electron detector.
+ Open protocol
+ Expand
4

Scanning Electron Microscopy of Foam Extrudate

Check if the same lab product or an alternative is used in the 5 most similar protocols
Scanning electron microscope (SEM) images were taken in order to determine the cell size, wall thickness, and general cell structure. Images were captured with JEOL JSM-6360LV (JEOL Ltd., Tokyo, Japan). A small section of the foam extrudate was frozen in liquid nitrogen and fractured. The fractured surfaces were then gold-coated with the Bal-Tec SCD050 (BalTec, Balzers, Liechtenstein) sputter coater. An acceleration voltage of 7 kV was used and the images were constructed from secondary electrons.
+ Open protocol
+ Expand
5

Scanning Electron Microscopy of Film Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
The microstructure of the film samples was observed by scanning electron microscopy using a JEOL-JSM 6360LV scanning electron microscope (Jeol, Ltd., Tokyo, Japan) operated at 15 kV. The working distance was 10 mm. Sample preparation was carried out as described by Díaz et al. [23 (link)]. Surface and cross-sectional images were taken at 200× and 1000× magnification, respectively. The roughness of film surfaces was measured from SEM images using ImageJ software version 1.54h (U.S. National Institutes of Health, Bethesda, MD, USA) [28 (link)].
+ Open protocol
+ Expand
6

Evaluating Debris Removal in Root Canals

Check if the same lab product or an alternative is used in the 5 most similar protocols
The method for the cleanliness evaluation was a modified version of Al-Hadlaq et al.
[18 (link)]. The teeth were removed from the stones and sectioned into two halves using a carborundum disk to create the longitudinal grooves on the buccal and lingual surfaces without entering the canals. The teeth were then split using a chisel and mallet. The most visible part of the canal was taken and divided into three main parts (coronal, middle, and apical) by creating three horizontal grooves and using a tapered carbide bur perpendicular to the canal. The samples were air-dried, sputter-coated with gold using a fine-coat ion sputter JFC-1100 (Fine coat ion sputter JFC-1100, JEOL Ltd., Tokyo, Japan), and then evaluated using Scanning Electron Microscope (SEM) (Jeol JSM-6360 LV, JEOL Ltd.). These three main parts were magnified up to X 20 magnification using SEM. Four random areas of each third were selected and magnified up to X 200 and then averaged to observe the debris layer removal from the canal walls. The captured images were analysed using ImageJ software (ImageJ 1.47 V, National Institute of Health, USA). The percentage of debris on the entire surface area was measured using the software to analyze the particles.
+ Open protocol
+ Expand
7

Microcapsule Morphology Analysis by SEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
The morphology of the microcapsules was observed using scanning electron microscopy (SEM; Jeol JSM-6360LV, JEOL Ltd., Akishima, Japan). Samples were mounted on double-sided tape, on aluminum stubs and sputter-coated with gold/palladium, and micrographs were taken at the appropriate magnification at the accelerating voltage of 15 KV. To observe the cross-section of microcapsules, particles were dispersed into ethyl-2-cyanoacrylate pellets and then sectioned using a razor blade.
+ Open protocol
+ Expand
8

Scanning Electron Microscopy of Fracture Characteristics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Morphological observations were carried out by scanning electron microscopy (SEM) using an SEM microscope Jeol JSM 6360LV (JEOL, Tokyo, Japan). All samples were coated with a thin layer of gold for electron conductivity. In addition, SEM observations were conducted on tested specimens to observe the fracture characteristics.
+ Open protocol
+ Expand
9

Scanning Electron Microscopy of Petal Epoxy Replicas

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mature petals of T. fournieri were dissected and taped on slide glasses. Multiple layers of impression materials were used to generate the epoxy replicas of petals as previously described in [38 ]. The epoxy replicas were sputtered with gold particles before observing under a scanning electron microscope Jeol JSM 6360LV (Jeol, Tokyo, Japan). The images were adjusted by Adobe PHOTOSHOP CS6 (Adobe, San Jose, CA, USA).
+ Open protocol
+ Expand
10

Scanning Electron Microscopy of Film Microstructure

Check if the same lab product or an alternative is used in the 5 most similar protocols
Film microstructure was analyzed by scanning electron microscopy using a JEOL-JSM 6360LV scanning electron microscope (Jeol Ltd., Tokyo, Japan) operated at 15 kV. Sample preparation was carried out as described by Díaz et al. [62 (link)].
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!