Cias latex crp h

All participants underwent a clinical examination and a standardized interview. Physical activity was evaluated based on the responses to questions regarding the types and frequencies of physical activity at work and during leisure time. Physical activity was classified as “≥4 times per week and≥20 minutes at a time,” “<80 minutes per week,” or “none.” Smoking and drinking status was classified as “never,” “former,” or “current” according to self-reported information. Monthly income per family member (at baseline) was categorized as “<¥600,” “¥600–799,” “¥800–999,” and “≥¥1,000.”
Anthropomorphic parameters such as height and weight and waist circumference were measured. The body mass index (BMI) was calculated as weight/height (kg/m²). Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured thrice in the seated position using a mercury sphygmomanometer, and the average of the 3 readings was used for the analyses.
Blood samples were collected from the antecubital vein after an overnight fast. Venous blood was obtained for determination of routine chemistry, including fasting blood glucose (FBG), high density lipoprotein-cholesterol (HDL-C), total cholesterol (TC), and triglycerides (TG). High sensitive C-reactive protein (hs-CRP) was measured by high-sensitivity nephelometry assay (Cias Latex CRP-H, Kanto Chemical, Tokyo, Japan).

Measurements of hs-CRP and LDL-c were performed biennially from during 2006-2007, 2008-2009, 2010-2011, and 2012-2013 to calculate cumulative burdens. Participant blood samples were collected after fasting for 8-12 h and transferred to vacuum tubes containing EDTA. All biochemical parameters were measured in the central laboratory of Kailuan General Hospital. LDL-c levels were measured by a direct testing method with an inter-assay coefficient of variation < 10% (Mind Bioengineering, Shanghai, China). Serum levels of hs-CRP were determined using a high-sensitivity nephelometry assay with a lower detection limit of 0.1 mg/L, an intra-assay coefficient of variation of 6.53%, and an inter-assay coefficient of variation of 4.78% (Cias Latex CRP-H, Kanto Chemical, Tokyo, Japan).

Blood samples were drawn from the antecubital vein in the morning at the fasting state and stored in vacuum tubes containing EDTA (Ethylene Dia mine Tetraacetic Acid), and all the blood tests were done at the central laboratory of the Kailuan hospital. Serum hs-CRP, creatinine, uric acid, total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and fasting blood glucose (FBG) were assessed. Hs-CRP was measured by high-sensitivity nephelometry assay (Cias Latex CRP-H, Kanto Chemical Co. Inc, Tokyo, Japan).

Whole blood samples were drawn from all participants, generally after an overnight fast and analyzed in the Central Laboratory of Kailuan General Hospital on the same day. Plasma samples were used to measure biochemical variables. Triglyceride, TCHO, hemoglobin, HDL, LDL were measured using a Hitachi 7600 auto-analyzer (Hitachi; Tokyo, Japan). Fasting blood glucose (FBG) was tested with the Hexokinase method (BioSino Bio-Technology & Science Inc., China). High sensitivity C reactive protein (hsCRP) was measured using a high-sensitivity particle-enhanced immunonephelometry assay (Cias Latex CRP-H, Kanto Chemical Co. Inc, Japan). Laboratory urine tests including urinary creatinine, urinary albumin, and urinary α1MG, transferrin and α1-acid glycoprotein that regarded as the indices of early DKD [17 (link)] were measured in the central laboratory in Peking University First Hospital. Serum creatinine was measured using the Jaffe’s method. eGFR was calculated using the CKD-EPI equation [24 (link)].

Hs‐CRP was measured with a high‐sensitivity nephelometry assay (Cias Latex CRP‐H, Kanto Chemical Co. Inc., Tokyo, Japan). The hs‐CRP concentrations were categorized into two groups as follows16: high level (hs‐CRP+, >3 mg/L) and low level (hs‐CRP−, <3 mg/L).
The plasma Lp‐PLA2 concentration was measured using a high‐sensitivity, quantitative sandwich enzyme‐linked immunosorbent assay (Quantikine ELISA, R&D Systems Inc. Minneapolis, MN, USA) and categorized into two groups as follows: high level (Lp‐PLA2+, >200 ng/mL) and low level (Lp‐PLA2−, ≤200 ng/mL).17All participants were divided into the following four groups: low hs‐CPR/low Lp‐PLA2 (hs‐CRP−/Lp‐PLA2−), high hs‐CRP/low Lp‐PLA2 (hs‐CRP+/Lp‐PLA2−), low hs‐CRP/high Lp‐PLA2 (hs‐CRP−/Lp‐PLA2+) and high hs‐CRP/high Lp‐PLA2 (hs‐CRP+/Lp‐PLA2+).