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Stereo discovery v8

Manufactured by Leica

The SteREO Discovery V8 is a stereomicroscope designed for a wide range of applications. It offers high-resolution imaging, a large field of view, and a comfortable viewing experience. The SteREO Discovery V8 features an advanced optical system that delivers clear, detailed images.

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2 protocols using stereo discovery v8

1

Taxonomic Identification of Cumacean Specimens

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A total of 947 specimens (Table S2) were determined to the lowest possible taxonomic rank, based primarily on original species descriptions (e.g., Hansen, 1920 (link); Sars, 1900 ). Species identifications were conducted at the Department of Biological Sciences (University of Bergen, Norway) and DZMB Hamburg using either a ZEISS SteREO Discovery V8 or Leica MZ12.5 dissecting microscope. Dissected pereopods and mouth parts were assessed under a ZEISS Primo Star compound microscope. High quality pictures with depth of focus were taken with a Leica DFC400 digital compound microscope camera using the Z-stacking option in the Leica Application Suite imaging software. Current authoritative classification follows the catalogue World Cumacea Database (http://www.marinespecies.org/cumacea/, Watling & Gerken, 2019 ) in the World Register of Marine Species (WoRMS Editorial Board, 2019 ). Additionally, comparative museums’ material has been obtained from the Center of Natural History Hamburg (CeNak) and the University Museum of Bergen (ZMBN).
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2

Fungal Isolation from Decomposing Bamboo

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Dead bamboo culms were collected from a freshwater stream from Krabi province, Thailand, in 2015. The samples were incubated in plastic boxes with sterile and moist tissue at 25–30 °C for 3 days. Pure fungal colonies were obtained using single-spore isolation [28 (link)]. Germinating spores were transferred aseptically to potato dextrose agar (PDA) and malt extract agar (MEA) (Difco™). The cultures were incubated at 25 °C with frequent observations. Fungal characters were observed using a stereo microscope (Zeiss SteREO Discovery v. 8) fitted with an Axio Cam ERc5S and a Leica DM2500 compound microscope attached with a Leica MC190 HD camera. All microscopic measurements were carried out using Tarosoft (R) Image Frame Work program and the images were processed with Adobe Photoshop CS6 version 13.0 software (Adobe Systems, San Jose, CA, USA). The type specimens were deposited in the Mae Fah Luang University (MFLU) Herbarium, Chiang Rai, Thailand, and pure cultures were deposited at the Mae Fah Luang University Culture Collection (MFLUCC). The new taxon was linked with Facesoffungi numbers (FoF) [29 (link)] and Index Fungorum (Index Fungorum 2020, http://www.indexfungorum.org/, accessed on 2 December 2020) and established based on guidelines recommended by Jeewon and Hyde [30 (link)].
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