Carbonic anhydrase from bovine erythrocytes (product code C2624, batch# SLBW5860, containing >3500 Wilbur-Anderson units/mg protein) and CO2 (99.998% purity) were purchased from Sigma-Aldrich (Algés, Portugal) and Praxair (Porto Maia, Portugal), respectively. Mineral water was purchased from a local market (Monchique brand, alkaline pH). Buffered conditions consisted of sodium carbonate-bicarbonate buffer 100 mM at pH 10.
Carbonic anhydrase from bovine erythrocytes
Manufactured by Merck Group
Sourced in United States, Portugal
Carbonic anhydrase from bovine erythrocytes is a laboratory reagent that consists of the enzyme carbonic anhydrase extracted from the red blood cells of cattle. Carbonic anhydrase is an enzyme that catalyzes the interconversion of carbon dioxide and water. This product is intended for use in various research and analytical applications that require the carbonic anhydrase enzyme.
Automatically generated - may contain errors
Lab products found in correlation
Siliaflash f60, by Silicycle (2 mentions)
Dnase 1, by Roche (1 mentions)
Crotonic anhydride, by Merck Group (1 mentions)
Bcecf am, by Merck Group (1 mentions)
Pyranine, by Merck Group (1 mentions)
Phycoerythrin pe, by Beckman Coulter (1 mentions)
Dids 4 4 2 diisothiocyanatostilbene 2 2 2 disulfonic acid disodium salt, by Merck Group (1 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
α casein from bovine milk, by Merck Group (1 mentions)
Standard proteins, by Merck Group (1 mentions)
Ribonuclease a from bovine pancreas, by Merck Group (1 mentions)
Trypsin inhibitor from glycine max soybean, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
β lactoglobulin from bovine milk, by Merck Group (1 mentions)
Optima lc ms grade formic acid, by Thermo Fisher Scientific (1 mentions)
Cytochrome c from equine heart, by Merck Group (1 mentions)
Ubiquitin from bovine erythrocytes, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Span 80, by Merck Group (1 mentions)
14 protocols using carbonic anhydrase from bovine erythrocytes
1
Carbonic Anhydrase Activity Assay
2
Crotonyl-CoA Synthesis and Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Crotonic Anhydride and Carbonic anhydrase from bovine erythrocytes were purchased from Sigma-Aldrich AG, CoA trilithium salt and DNase I from Roche Diagnostics, and NADPH Na4 (98%) from Carl Roth GmbH. Solvents and salts were all analytical grade or better. Crotonyl-CoA was synthesized as previously reported (30 (link)).
3
Antibody sources and fluorescent probes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rabbit polyclonal antibody anti-AE1 was kindly provided by Dr C. Wagner (University of Zürich, Switzerland). Mouse monoclonal antibody anti-AE1 (BRIC6) came from the International Blood Group Reference Laboratory (IBGRL, Bristol, United Kingdom). Mouse monoclonal antibodies anti-ankyrin R (clone N388A/10) and anti-AQP1 (clone 1/A5F6) were from Neuromab (UC Davis/NIH, USA) and Bio-Rad (Marnes-la-Coquette, France) respectively, while goat polyclonal anti-stomatin (M-14) and rabbit polyclonal anti-CAII antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas USA). Secondary antibodies used in flow cytometry analysis were phycoerythrin (PE) or fluorescein isothiocyanate (FITC)-conjugated F(ab’)2 fragment of goat anti-mouse and donkey anti-goat immunoglobulins from Beckman Coulter (Villepinte, France). BCECF-AM [2′,7′bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester] and pyranine [8-hydroxypyrene-1,3,6-trisulfonic acid] fluorescent probes, as well as carbonic anhydrase from bovine erythrocytes and the AE1 inhibitor DIDS [4,4 2-Diisothiocyanatostilbene-2,2 2-disulfonic acid disodium salt] were purchased from Sigma-Aldrich (Saint Quentin, France). Chloride indicator SPQ (6-methoxy-N-(3-sulfopropy1) Quinolinium) was obtained from Invitrogen (Fisher Scientific, Illkirch, France).
4
LC-MS Protein Separation Workflow
5
Protein Purification and Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Standard
proteins, ribonuclease
A from bovine pancreas, trypsin inhibitor from Glycine max (soybean) and carbonic anhydrase from bovine erythrocytes were purchased
from Sigma Chemical (St. Louis, MO). Escherichia coli protein sample (lyophilized control sample for use in IEF applications)
was purchased from Bio-Rad (Hercules, CA) and dissolved in water.
An Amicon spin filter (cutoff 100k) from Millipore (Billerica, MA)
was used to remove large species in the sample. HPLC gradient acetonitrile
and water were purchased from Sigma (St. Louis, MO) and used all the
time.
proteins, ribonuclease
A from bovine pancreas, trypsin inhibitor from Glycine max (soybean) and carbonic anhydrase from bovine erythrocytes were purchased
from Sigma Chemical (St. Louis, MO). Escherichia coli protein sample (lyophilized control sample for use in IEF applications)
was purchased from Bio-Rad (Hercules, CA) and dissolved in water.
An Amicon spin filter (cutoff 100k) from Millipore (Billerica, MA)
was used to remove large species in the sample. HPLC gradient acetonitrile
and water were purchased from Sigma (St. Louis, MO) and used all the
time.
6
Protein Standard Preparation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optima LC/MS grade formic acid, methanol, and water were obtained from Fisher Scientific (Waltham, MA). Ubiquitin from bovine erythrocytes, cytochrome C from equine heart, β-lactoglobulin from bovine milk, carbonic anhydrase from bovine erythrocytes, and bovine serum albumin were obtained from Sigma Aldrich (St. Louis, MO).
7
Emulsification and Membrane Screening for CO2 Capture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The commercial oils screened were olive oil (Gallo, Alges, Portugal), sunflower oil (Fula, Alges, Portugal) and corn oil (Fula, Alges, Portugal). Surfactants used for emulsification were PEG 300 (Clariant, Muttenz, Switzerland), Span® 80, Tween® 80, Triton™ X-100 Detergent and SDS (≥99% (GC), dust-free pellets), which were procured from Sigma Aldrich, Portugal (made in Lyon, France). Potassium carbonate (>99.5% pure, Scharlab S.L., Sentmenat, Spain) was used to prepare a solution to adjust the pH to 11. Carbonic anhydrase from bovine erythrocytes (≥95% (SDS-PAGE), specific activity ≥3500 W-A units/mg protein, lyophilised powder) was acquired from Sigma Aldrich, Portugal (made in St. Louis, MI, USA). The gases used, carbon dioxide (98% purity) and methane (99% purity) were purchased from Praxair (Huelva, Spain). The commercial membranes used in this study are detailed in Table 1 .
8
Protein Concentration Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GdnHCl, p -nitrophenyl acetate, acetone, ethanol, and hydrochloric acid were purchased from Wako Pure Chemical Industries (Osaka, Japan). Coomassie Plus (Bradford) Assay TM kits for measuring the protein concentration were purchased from Thermo Fisher Scientific K.K.
(Rockford, USA). Carbonic Anhydrase from bovine erythrocytes was purchased from Sigma-Aldrich Co. LLC (St. Louis, USA). Sylgard184 was obtained from Dow Corning Corp. (Midland, USA), and cellulose tubes (MWCO 12-14 kDa, pore size 5 nm, thickness 39.2 μm, diameter 28.6 mm) were purchased from Nihon Medical Science, Inc. (Tokyo, Japan).
(Rockford, USA). Carbonic Anhydrase from bovine erythrocytes was purchased from Sigma-Aldrich Co. LLC (St. Louis, USA). Sylgard184 was obtained from Dow Corning Corp. (Midland, USA), and cellulose tubes (MWCO 12-14 kDa, pore size 5 nm, thickness 39.2 μm, diameter 28.6 mm) were purchased from Nihon Medical Science, Inc. (Tokyo, Japan).
9
Capillary-Based Protein Separation and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fused-silica capillary tubings of 0.250, 0.200 and 0.075 mm I.D. (0.375 mm O.D.) with a polyimide outer coating were purchased from Polymicro Technologies (Phoenix, AZ, USA). Dorica Supporting devices (figure S1) to protect the capillary columns were from Avantech Group s.r.l (Angri, SA, Italy). Azoisobutyronitrile (AIBN), acetonitrile (ACN), trifluoroacetic acid (TFA), lauryl methacrylate (LMA), 1,6-hexanediol dimethacrylate (HDDMA), tert-butyl alcohol, 1,4butanediol, tetrahydrofuran (THF), methacrylic anhydride, pyridine, sodium hydroxide (NaOH), hydrochloric acid (HCl), ammonium bicarbonate, uracil, phenol, benzaldehyde, nitro-benzene, benzene, toluene, ethyl-benzene, n-propyl-benzene, n-butyl-benzene, n-pentyl-benzene as well as lysozyme from chicken egg white, -lactalbumin from bovine milk, -lactoglobulin B from bovine milk, carbonic anhydrase from bovine erythrocytes, core histones from calf thymus were purchased from Sigma-Aldrich (St. Louis, MO, USA). Porcine trypsin was purchased from Promega (Madison, WI, USA). (N-trimethoxysilylpropyl)-polyethylenimine from United Chemical Technologies (Bristol, PA, USA) was used as a silanization reagent.
10
Synthesis and Characterization of Peroxynicotinoid Compounds
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!