Human mesenchymal stem cells (hMSCs, StemCell Technologies) were cultured using Mesencult™ proliferation kit supplemented with 2mM glutamax and 1% penicillin-streptomycin (P/S). Human cardiomyocytes (CMs, PromoCell) were cultured in myocyte growth medium kit following the manufacturer’s instruction. Cells were maintained in a humidified cell culture incubator at 37°C and 5% CO2 with respective growth medium. Cell culture medium was changed every other day. All experiments were performed using hMSCs at passage 3~7 and human CMs at passages 6-9.
Hmscs
Manufactured by STEMCELL
Sourced in United Kingdom
HMSCs are primary human mesenchymal stem cells derived from healthy adult bone marrow. They are capable of differentiation into various cell types, including adipocytes, osteoblasts, and chondrocytes.
Automatically generated - may contain errors
2 protocols using hmscs
1
Culturing Human Mesenchymal Stem Cells and Cardiomyocytes
2
Mesenchymal Stem Cell Culturing and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hMSCs were purchased from Stem Cell Technologies and cultured in DMEM (1 g/l glucose; Invitrogen, Paisley, U.K.) containing 10% fetal bovine serum (Invitrogen, Paisley, U.K.), and 1% penicillin and streptomycin (Thermo Scientific, Loughborough, U.K.). Cells were expanded from the same stocks as we have used previously 7 whereby the vendor had shown a >90% expression of CD29, CD44, CD105, and CD166 and <1% expression of CD14, CD34, and CD45. hMSCs were split at 75%–90% confluence and used between passages 9 and 14. For transplantation studies, hMSCs expression of the mesenchymal markers CD105 and CD73 was verified (Supporting Information Fig. 1D) and the cells were resuspended in explant medium prior to culture on the RGC layer of the retina. For conditioned medium investigation, hMSCs were grown in T75 tissue culture flasks (Corning, NY) and transferred to serum free medium for 72 hours.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!