Hdac activity assay kit

Manufactured by Epigentek

Sourced in United States

The HDAC activity assay kit is a laboratory equipment product designed to measure the activity of histone deacetylase (HDAC) enzymes. It provides a simple and efficient method for quantifying HDAC activity in cell or tissue samples.

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Lab products found in correlation

Panobinostat, by Selleck Chemicals (2 mentions) Vorinostat, by Selleck Chemicals (2 mentions) Romidepsin, by Selleck Chemicals (2 mentions) Nuclear extraction kit, by Active Motif (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions) Trypsin, by Thermo Fisher Scientific (2 mentions) Lc ms grade water, by Thermo Fisher Scientific (2 mentions) Tmtpro 16plex label reagent set, by Thermo Fisher Scientific (2 mentions) Sigma 1 g c18 seppak cartridges, by Waters Corporation (2 mentions) Dithiothreitol (dtt), by Thermo Fisher Scientific (2 mentions) Mtt assay kit, by Abcam (2 mentions) Bca assay kit, by Thermo Fisher Scientific (2 mentions) Iodoacetamide, by Merck Group (2 mentions) Nuclear extract kit, by Abcam (1 mentions) Hat activity assay kit, by Epigentek (1 mentions)

Close spelling variants of this product

EpiQuik HDAC Activity/Inhibition Assay Kit Epigenase HDAC Activity/Inhibition Direct Assay Kit Epigenase HDAC Activity Assay Kit EpiQuik HDAC Activity Assay Kit HDAC Activity/Inhibition Direct Assay Kit HDAC activity/inhibition assay kit EpiQuick HDAC Activity/inhibition Assay Kit Epigenase HDAC Activity Direct Assay Kit

5 protocols using hdac activity assay kit

Nuclear Protein HDAC1 Activity Assay

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Nuclear proteins were extracted with a Nuclear Extract Kit (Abcam). The HDAC1 activity was subsequently measured with an HDAC Activity Assay Kit (Epigentek, Farmingdale, NY, USA). All procedures were conducted according to the manufacturer's recommendations.

Shinke G., Yamada D., Eguchi H., Iwagami Y., Asaoka T., Noda T., Wada H., Kawamoto K., Gotoh K., Kobayashi S., Takeda Y., Tanemura M., Mori M, & Doki Y. (2018). Role of histone deacetylase 1 in distant metastasis of pancreatic ductal cancer. Cancer Science, 109(8), 2520-2531.

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Quantifying HDAC Activity in FAPs

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HDAC activity in FAPs was measured by HDAC activity assay kit (Epigentek Inc., # P-4002–96). FAPs were collected with FACS and then lyzed with nuclear extract lysis buffer. The same amount of nuclear proteins from FAPs were was added into 96 well plates for HADC enzyme activity measurement by a Bio-rad plate reader (Bio-Rad Inc.) with 560 nm wavelength according to manufacture′s instruction.

Liu X., Liu M., Lee L., Davies M., Wang Z., Kim H, & Feeley B.T. (2020). Trichostatin A regulates fibro/adipogenic progenitor adipogenesis epigenetically and reduces rotator cuff muscle fatty infiltration. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 39(7), 1452-1462.

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Quantitative Proteomics of HDAC Inhibitors

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Romidepsin (S3020), Panobinostat (S1030), and Vorinostat (SAHA) (S1047) were purchased from Selleck Chemicals. Nuclear Extraction kit (40010) was purchased from Active Motif. HDAC Activity Assay kit (P-4034–96) was purchased from Epigentek. MTT assay kit was purchased from Abcam (ab211091). TMTpro 16plex Label Reagent set (A44522), trypsin, BCA assay kit and DTT were purchased from Thermo Fisher Scientific. LC/MS-grade water and formic acid were purchased from Fisher Scientific. Iodoacetamide (IAA) was purchased from Millipore Sigma.1 g C18 SepPak cartridges were purchased from Waters.

Duda J.M., Twigg C.A, & Thomas S.N. (2022). Differential histone deacetylase inhibitor-induced perturbations of the global proteome landscape in the setting of high-grade serous ovarian cancer. Proteomics, 23(3-4), e2100372.

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Epigenetic Enzyme Activity Assays

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The activity of HAT was assayed using a HAT activity assay kit (p-4003, Epigentek, NY, USA), and the activity of HDACs was assayed using a HDAC activity assay kit (P-4034, Epigentek, USA), following the manufacturer’s instructions.

Liu E., Zhou Q., Xie A.J., Li M., Zhang S., Huang H., Liuyang Z., Wang Y., Liu B., Li X., Sun D., Wei Y., Wang X., Wang Q., Ke D., Yang X., Yang Y, & Wang J.Z. (2019). Enriched gestation activates the IGF pathway to evoke embryo-adult benefits to prevent Alzheimer’s disease. Translational Neurodegeneration, 8, 8.

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Quantitative Proteomics of HDAC Inhibitors

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