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Anti mouse or anti rabbit horseradish peroxidase hrp conjugated secondary antibodies

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Anti-mouse or anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies are laboratory reagents used in immunodetection techniques. They bind to primary antibodies targeting mouse or rabbit antigens and catalyze a colorimetric reaction when exposed to a substrate, enabling the visualization and quantification of target proteins.

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Lab products found in correlation

β actin, by Merck Group (3 mentions) Nanog, by Cell Signaling Technology (2 mentions) Ontarget plus non targeting pool, by OriGene (2 mentions) Stat3, by Cell Signaling Technology (2 mentions) Anti pak4, by Cell Signaling Technology (2 mentions) P stat3 y705, by Cell Signaling Technology (2 mentions) Pvdf membrane, by Merck Group (2 mentions) X ray film, by AGFA HealthCare (2 mentions) 5loading buffer, by Thermo Fisher Scientific (2 mentions) Enhanced chemiluminescence detection system, by Cytiva (2 mentions) Rabbit anti β actin, by Cell Signaling Technology (2 mentions) Prl tk plasmid, by Promega (1 mentions) Mouse anti β catenin, by Santa Cruz Biotechnology (1 mentions) Rabbit anti dkk1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Horseradish peroxidase-conjugated (HRP) anti-rabbit antibodies (11 citations) Horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (9 citations) Horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibodies (5 citations) HRP (horseradish peroxidase)-conjugated secondary antibodies (5 citations) Anti-mouse or anti-rabbit HRP-conjugated secondary antibodies (4 citations) HRP-conjugated anti-rabbit or anti-mouse antibodies (4 citations) Horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-rabbit) (2 citations) HRP conjugated (mouse or rabbit) secondary antibodies (2 citations) Peroxidase-conjugated anti-mouse secondary antibodies (2 citations) Horseradish peroxidase (HRP) anti-rabbit (2 citations)

5 protocols using anti mouse or anti rabbit horseradish peroxidase hrp conjugated secondary antibodies

1

Isolation and Characterization of Cancer Stem Cells

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Anti-PAK4 (rabbit polyclonal), -Sox2, -Nanog, -pSTAT3 (Y705) (rabbit monoclonal) and -STAT3 (mouse monoclonal) antibodies were purchased from Cell Signaling Technology (Beverly, MA). Anti-KLF4, -Oct4 (mouse monoclonal) were procured from Abcam (Cambridge, MA). Antibodies targeting Lamin A, α-tubulin (mouse mono-clonal) and respective anti-mouse or anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies were procured from Santa Cruz Biotechnology (Santa Cruz, CA). β-actin (mouse monoclonal) antibody was purchased from Sigma-Aldrich (St. Louis MO). For isolation of cancer stem-like cells, anti-human fluorochrome-conjugated antibodies against CD24 (Alexa Fluor-647 conjugated), CD44 (Brilliant violent-421 conjugated) and EpCAM (also known as ESA) (Phycoerythrin/cy7 conjugated) were procured from BioLegend (San Diego, CA). The construct EF.STAT3C.Ubc.GFP (Addgene plasmid #24983) was from Linzhao Cheng laboratory and the control vector FUGW (Addgene plasmid #14883) was from David Baltimore laboratory and both the plasmids were procured from Addgene. All non-target (ONTARGET plus Non-targeting pool) and target-specific (ONTARGET plus SMART pool) siRNAs were from Origene (Rockville, MD).
Tyagi N., Marimuthu S., Bhardwaj A., Deshmukh S.K., Srivastava S.K., Singh A.P., McClellan S., Carter J.E, & Singh S. (2015). p-21 activated kinase 4 (PAK4) maintains stem cell-like phenotypes in pancreatic cancer cells through activation of STAT3 signaling. Cancer letters, 370(2), 260-267.
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2

Antibody Procurement and Plasmid Sources

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Anti -PAK4, -Bax, -Bcl2, -Cyclin D1, -p-IκB-α (Ser32/36), (rabbit polyclonal), -Bcl-xL, -NF-κB/p65, -ERK1/2 (rabbit monoclona1), -IκB-α, -p-ERK1/2 (mouse monoclonal) antibodies were purchased from Cell Signaling Technology (Beverly, MA). Antibodies against Akt and p-Akt (rabbit monoclonal) were from Epitomics (Burlingame, CA). Antibodies targeting Cyclin E1, p21, Laminin, α-tubulin (mouse monoclonal), p27, Cyclin A1 (rabbit polyclonal) and respective anti-mouse or anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies were procured from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-PAK4 antibody (rabbit polyclonal; for immunohistochemistry) was purchased from Pierce Biotechnology (Rockford, IL). β-actin (mouse monoclonal) antibody was purchased from Sigma-Aldrich (St. Louis MO). Plasmids expressing PAK4-targeting (PAK4-shRNA-pGFP-V-RS) and non-targeted scrambled (NTScr-shRNA-pGFP-V-RS) short hairpin RNA sequence and empty vector (pCMV) were purchased from Origene (Rockville, MD). pGL4.32 [luc2P/NF-B-RE/Hygro] and pRL-TK plasmids were from Promega (Madison, WI). pCMV-IKKβ S177E S181E (plasmid number 11105) was from A. Rao Laboratory and procured through Addgene (Cambridge, MA).
Tyagi N., Bhardwaj A., Singh A.P., McClellan S., Carter J.E, & Singh S. (2014). p-21 activated kinase 4 promotes proliferation and survival of pancreatic cancer cells through AKT- and ERK-dependent activation of NF-κB pathway. Oncotarget, 5(18), 8778-8789.
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3

Isolation and Characterization of Cancer Stem Cells

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Anti-PAK4 (rabbit polyclonal), -Sox2, -Nanog, -pSTAT3 (Y705) (rabbit monoclonal) and -STAT3 (mouse monoclonal) antibodies were purchased from Cell Signaling Technology (Beverly, MA). Anti-KLF4, -Oct4 (mouse monoclonal) were procured from Abcam (Cambridge, MA). Antibodies targeting Lamin A, α-tubulin (mouse mono-clonal) and respective anti-mouse or anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies were procured from Santa Cruz Biotechnology (Santa Cruz, CA). β-actin (mouse monoclonal) antibody was purchased from Sigma-Aldrich (St. Louis MO). For isolation of cancer stem-like cells, anti-human fluorochrome-conjugated antibodies against CD24 (Alexa Fluor-647 conjugated), CD44 (Brilliant violent-421 conjugated) and EpCAM (also known as ESA) (Phycoerythrin/cy7 conjugated) were procured from BioLegend (San Diego, CA). The construct EF.STAT3C.Ubc.GFP (Addgene plasmid #24983) was from Linzhao Cheng laboratory and the control vector FUGW (Addgene plasmid #14883) was from David Baltimore laboratory and both the plasmids were procured from Addgene. All non-target (ONTARGET plus Non-targeting pool) and target-specific (ONTARGET plus SMART pool) siRNAs were from Origene (Rockville, MD).
Tyagi N., Marimuthu S., Bhardwaj A., Deshmukh S.K., Srivastava S.K., Singh A.P., McClellan S., Carter J.E, & Singh S. (2015). p-21 activated kinase 4 (PAK4) maintains stem cell-like phenotypes in pancreatic cancer cells through activation of STAT3 signaling. Cancer letters, 370(2), 260-267.
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4

Western Blot Protein Detection

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Cell lysates in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (1 mM phenylmethylsulfonyl fluoride, 10 µg/ml leupeptin, 10 µg/ml pepstatin A, and 10 µg/ml aprotinin) were mixed with 5loading buffer (Fermentas, Waltham, MA, USA) and heated at 95°C for 5 min. Samples were separated electrophoretically on 8% sodium dodecyl sulfate (SDS)-polyacrylamide gels, and the separated proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Billerica, MA, USA). Membranes were blocked and probed with the following antibodies: rabbit anti-DKK1 (1:500; Cell Signaling Technology, Danvers, MA, USA), mouse anti-β-catenin (1:500; Santa Cruz Biotechnology), and rabbit anti-β-actin (1:3,000; Cell Signaling Technology). Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Santa Cruz Biotechnology) at a dilution of 1:3,000 for 45 min at room temperature. Protein bands were visualized using an enhanced chemiluminescence detection system (Amersham Bioscience, GE Healthcare, Piscataway, NJ, USA), and the membrane was exposed to X-ray film (Agfa, Mortsel, Belgium). Anti-β-actin antibody was used to confirm that loading was comparable between gel lanes. The density of each protein band was read and quantified using ImageJ software.
Min K, & Lee S.K. (2019). EBV miR-BART10-3p Promotes Cell Proliferation and Migration by Targeting DKK1. International Journal of Biological Sciences, 15(3), 657-667.
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5

Western Blot Analysis of DAB2 Protein

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Cell lysate in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (1 mM phenylmethylsulfonyl fluoride, 10 μg/ml leupeptin, 10 μg/ml pepstatin A, and 10 μg/ml aprotinin) was mixed with 5 loading buffer (Fermentas, Waltham, MA, USA) and heated at 95 °C for 5 min. Samples were separated electrophoretically on 8% sodium dodecyl sulfate (SDS)-polyacrylamide gels, and the separated proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Billerica, MA, USA). Membranes were blocked and probed with: mouse anti-DAB2 (1:1,000; BD Biosciences, San Jose, CA, USA), or rabbit anti-β-actin (1:3,000; Cell Signaling Technology, Danvers, MA, USA) antibodies. Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Santa Cruz, Dallas, TX, USA) at a dilution of 1:5,000 for 45 min at room temperature. Protein bands were visualized using an enhanced chemiluminescence detection system (Amersham Biosciences), and the membrane was exposed to X-ray film (Agfa, Mortsel, Belgium). Anti-β-actin antibody was used to confirm that loading was comparable between gel lanes. The density of each protein band was read and quantified using ImageJ software.
Min K., Kim J.Y, & Lee S.K. (2020). Epstein-Barr virus miR-BART1-3p suppresses apoptosis and promotes migration of gastric carcinoma cells by targeting DAB2. International Journal of Biological Sciences, 16(4), 694-707.
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