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Serum protein substitute

Manufactured by Merck Group

Serum protein substitute is a laboratory product that serves as a replacement for serum proteins. Its core function is to provide a source of proteins and other biomolecules that are typically found in serum, which can be used in various experimental and analytical procedures.

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2 protocols using serum protein substitute

1

Rapid Thawing and Culture of Human Blastocysts

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Human embryos cryopreserved at the blastocyst stage were thawed by a two-step rapid thawing protocol using Quinn's Advantage Thaw Kit (CooperSurgical, Trumbull, CT) as previously described35 ,36 . In brief, either cryostraws or vials were removed from the liquid nitrogen and exposed to air before incubating in a 37 °C water bath. Once thawed, embryos were transferred to a 0.5 mol l−1sucrose solution for 10 min followed by a 0.2 mol l−sucrose solution for an additional 10 min. The embryos were then washed in Quinn’s Advantage Medium with Hepes (CooperSurgical) plus 5% serum protein substitute (CooperSurgical) and each transferred to a 25 µl microdrop of either Quinn’s advantage cleavage medium (CooperSurgical) or Quinn’s advantage cleavage medium (CooperSurgical) supplemented with 10% serum protein substitute under mineral oil (Sigma, St Louis, MO). The embryos were cultured at 37 °C with 6% CO2, 5% O2 and 89% N2 under standard human embryo culture conditions in accordance with current clinical IVF practice. Embryos used in this study were days post fertilization (DPF) 5–6.
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2

Rapid Thawing and Culture of Human Blastocysts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human embryos cryopreserved at the blastocyst stage were thawed by a two-step rapid thawing protocol using Quinn's Advantage Thaw Kit (CooperSurgical, Trumbull, CT) as previously described35 ,36 . In brief, either cryostraws or vials were removed from the liquid nitrogen and exposed to air before incubating in a 37 °C water bath. Once thawed, embryos were transferred to a 0.5 mol l−1sucrose solution for 10 min followed by a 0.2 mol l−sucrose solution for an additional 10 min. The embryos were then washed in Quinn’s Advantage Medium with Hepes (CooperSurgical) plus 5% serum protein substitute (CooperSurgical) and each transferred to a 25 µl microdrop of either Quinn’s advantage cleavage medium (CooperSurgical) or Quinn’s advantage cleavage medium (CooperSurgical) supplemented with 10% serum protein substitute under mineral oil (Sigma, St Louis, MO). The embryos were cultured at 37 °C with 6% CO2, 5% O2 and 89% N2 under standard human embryo culture conditions in accordance with current clinical IVF practice. Embryos used in this study were days post fertilization (DPF) 5–6.
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