The locomotor activity was measured using the open field locomotor system (Omnitech Electronics Inc, Columbus, OH). The test apparatus consisted of an open field box (40 × 40 × 30cm) made of transparent acrylic surrounded by 3 sets of 16 photobeam arrays in the horizontal x and y axes as well as in the ventricle z axis. Locomotor movements were determined by breaks in photobeams and converted into distance traveled with the Fusion Software. Total distance traveled for 15 minutes was analyzed using the Fusion Software (Omnitech Electronics).
Fusion software
Manufactured by Omnitech Electronics
Sourced in United States
Fusion software is a comprehensive data analysis and visualization tool designed for laboratory applications. It provides a suite of advanced features for processing, analyzing, and presenting experimental data from various sources. The software's core function is to enable researchers and scientists to efficiently manage, manipulate, and interpret their experimental data in a user-friendly environment.
Automatically generated - may contain errors
Lab products found in correlation
Superflex fusion, by Omnitech Electronics (5 mentions)
Photo beam sensors, by Omnitech Electronics (3 mentions)
Open field locomotor system, by Omnitech Electronics (2 mentions)
Open field apparatus, by Omnitech Electronics (2 mentions)
Locomotor activity apparatus, by Omnitech Electronics (2 mentions)
Superflex fusion open field, by Omnitech Electronics (2 mentions)
Vanilla ensure, by Abbott (1 mentions)
Homecagescan, by CleverSys (1 mentions)
Superflex open field cages, by Omnitech Electronics (1 mentions)
37 protocols using fusion software
1
Open Field Locomotor Activity Assessment
2
Open Field Test for Locomotion and Anxiety
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For locomotion and anxiety-like behaviors, mice were placed in an open field apparatus (Omnitech Electronics, Inc., Columbus, OH), which consisted of a 16 × 16-in. plastic chamber surrounded by 16 photobeam detectors along the x- and y-axis to measure horizontal movement. Mice were placed in the open field apparatus for 30 min and distance and time in center vs. periphery were recorded. Behavior was analyzed using Fusion software (Version 5.6, Omnitech Electronics, Inc.).
3
Open Field Test of Rodent Behavior
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Open field tests are often used to measure locomotor activity and anxiety-like behaviors in rodents (53 (link)–55 (link)). The rats' ambulatory behavior was measured using a locomotor activity apparatus (Omnitech Electronics, Columbus, OH). For this assessment, one animal at a time was placed in the center of the open field arena (50 × 50 × 30 cm) and allowed to freely explore the inside for one h. Activities such as horizontal activity, vertical activity, ambulatory activity, total distance moved, and time spent/distance traveled at the center and margins of the apparatus were measured using Fusion Software (Omnitech Electronics, Columbus, OH). The open field explorations were monitored on days 1, 7, 14, and 28 post-blast exposures. A total of 9 to 12 rats per group for different time-points were used for this test.
4
Liquid Diet Feeding Assay in Mice
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For feeding assays, mice were individually housed in specialized food/liquid intake measurement cages attached to water bottles mounted on scales (DietMax, Omnitech Electronics). Mice were provided a liquid diet of Vanilla Ensure (Abbott Laboratories) diluted in a 1:1 ratio with water for a total caloric density of 450 kcal/L. Bottles containing liquid diet were washed and disinfected daily and fully replenished at the beginning of the light cycle. Mice were also provided ad libitum access to HydroGel (ClearH2O, #70-01-5022) to ensure constant hydration. Individual feeding bouts were recorded using scale measurements (Fusion Software, Omnitech Electronics). A meal was defined as any linear decrease greater than 0.02 g of liquid Ensure. The minimum time between two meals was 15 s (otherwise, only one meal was recorded). Mice were allowed to habituate for a minimum of 72 hr prior to the beginning of experiments, and all food intake measurements were obtained during the middle 4 hr of the inactive cycle.
5
Open Field Exploration Analysis
6
Assessing Locomotor and Vertical Activity in Mice
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Mice were assessed for horizontal activity (distance traveled, cm) and the number of rearing episodes (vertical activity), which requires hindlimb loading.40 (link) Animals were placed individually in plexiglass boxes (16 × 16 × 11.75 inches) containing a thin layer of bedding and a 1-inch square of Napa Nectar (Systems Engineering, Napa, CA). Horizontal and vertical activities were assessed via open field monitoring by arrays of photobeam sensors that use beam breaks to determine the location of each animal at all times (Omnitech Electronics, Columbus, OH) (Supplemental Figure 1, available online at http://links.lww.com/PR9/A7 ). Horizontal locomotor activity and the number of rearing episodes were continuously monitored for 20 hours beginning at 1200 hours (noon) in a light- and temperature-controlled testing room that remained closed to any other activity. Fusion software (Omnitech Electronics) was used to analyze and store the above parameters.
7
Open-Field Locomotor Activity Assay
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The locomotor activity was measured in a SuperFlex Fusion open field cage (40 cm × 40 cm × 30 cm, Omnitech Electronics Inc., Columbus, OH). Individual mice was allowed to acclimate to the single cage for 2–4 h prior to the test. The animals were gently placed in the center of the test apparatus (40 cm × 40 cm × 40 cm) and allowed to explore the field for 30 min. Locomotor activity of the mice was monitored and recorded with infrared motion sensors mounted on top of the cage. The total distance traveled was analyzed using Fusion software (Omnitech Electronics Inc., Columbus, OH).
8
Assessing Locomotion, Stereotypy, and Anxiety-like Behaviors in Mice
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To determine changes to locomotive, stereotyped and anxiety‐like behaviors, all subjects underwent the open field test.29 The testing environment consisted of a Fusion Node acrylic arena (40 cm × 40 cm × 30 cm) (Omnitech Electronics, Columbus, OH, United States). Individual mice were placed in the arena for 30 min. Photobeams at the edge of each zone detected activity within the arena using Fusion Software (Omnitech Electronics). Total distance traveled, rearing frequency and rearing time were analyzed as measures of exploratory activity and locomotive behavior. Circling behavior and time spent grooming (stereotypy time) determined differences in stereotyped behavior. Both the distance traveled, and time spent in the center (inner 50%, 20 cm × 20 cm region) of the open field were compared between groups to evaluate the presence of anxiety‐like and exploratory behavior.
9
Locomotor Activity Monitoring in Mice
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All rodent behavioral analyses used approximately equal numbers of 10-month-old male littermates at the time of testing. Mice were placed in the testing room at least 30 min before each assay. All experiments and analyses were performed completely in a genotype-blind manner. Locomotor data was collected using an Accuscan Instruments behavioral monitoring system and Fusion software (Omnitech Electronics). Mice were temporarily single-housed on a 12 hr light-dark cycle with food and water ad libitum. Nesting material was removed to prevent obstruction of infrared beams in the locomotor monitoring system. Locomotion was monitored for 72 hr using 12 photocells placed 4 cm apart. Locomotor counts were monitored in 60 s blocks to obtain an “ambulatory activity count” consisting of the number of beam breaks recorded during a period of ambulatory activity. Mice were not disturbed during the testing period. Raw data from the Fusion software was then post-processed using a custom MATLAB script to extract the first 3 hr of data and re-organize into 15 min time bins.
10
Open Field Locomotion Assay
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