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Anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody

Manufactured by GeneTex
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Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody is a laboratory reagent used to detect the presence of the GAPDH protein in biological samples. GAPDH is a key enzyme involved in glycolysis, a fundamental metabolic process. This antibody can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of GAPDH in cells and tissues.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Cell death detection elisaplus kit, by Roche (2 mentions) Anti cxcr4 antibody, by Abcam (1 mentions) Anti actin antibody, by Merck Group (1 mentions) Biotin conjugated goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Ndufs1, by Proteintech (1 mentions) Ndufv1, by Proteintech (1 mentions) Anti cytochrome c antibody, by Abcam (1 mentions) Annexin 5 fitc propidium iodide apoptosis detection kit, by BD (1 mentions) Mitotracker green, by Thermo Fisher Scientific (1 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions) Withaferin a, by ChromaDex (1 mentions) Human apoptosis antibody array, by Abcam (1 mentions) Tumor necrosis factor α tnf α, by R&D Systems (1 mentions) Sb202190, by Enzo Life Sciences (1 mentions) Hybond c membrane, by GE Healthcare (1 mentions) Phospho p38 thr180 tyr182, by Cell Signaling Technology (1 mentions) Phospho jnk1 2 thr183 tyr185, by Cell Signaling Technology (1 mentions) U0126, by Enzo Life Sciences (1 mentions)

Close spelling variants of this product

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody Anti-GAPDH antibody GAPDH antibody Anti-GAPDH GAPDH

8 protocols using anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody

1

Investigating CXCR4 and AKT Signaling in Prostate Cancer

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Majority of the cell culture reagents were purchased from Invitrogen-Life Technologies, whereas RPMI 1640 medium was from Mediatech. Sources of the antibodies were as follows: anti-CXCR4 antibody was from Abcam, an antibody specific for detection of S473 phosphorylated AKT was from Cell Signaling Technology; anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was from GeneTex; antibodies against phospho- and total extracellular-signal regulated kinases (ERK) were purchased from Santa Cruz Biotechnology, and anti-actin antibody was from Sigma-Aldrich. Transwell Permeable Support (8 µm polycarbonate membrane) chambers were purchased from Corning. Small interfering RNA for knockdown of CXCR4 was purchased from Santa Cruz Biotechnology. Stock solutions of PEITC, BITC, and SFN (purity ≥98%; structures are shown in Fig. 1A) were stored at −20°C and diluted immediately before use. LNCaP, C4-2, 22Rv1, and PC-3 human prostate cancer cells were acquired from the American Type Culture Collection and last authenticated in 2012. Each cell line was found to be of human origin and free of pathogen contamination. PC-3 cells stably transfected with CXCR4 plasmid (hereafter abbreviated as CXCR4_PC-3) or empty vector (Neo_PC-3) have been described previously (27 (link)).
Sakao K., Vyas A.R., Chinni S.R., Amjad A.I., Parikh R, & Singh S.V. (2015). CXCR4 Is a Novel Target of Cancer Chemopreventative Isothiocyanates in Prostate Cancer Cells. Cancer prevention research (Philadelphia, Pa.), 8(5), 365-374.
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2

Antibody Validation for Collagen Types

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Rabbit polyclonal antibodies against Atf6 and Type I collagen were purchased from Abcam (Cambridge, UK). Rabbit polyclonal antibodies against Type III collagen were from Santa Cruz (Dallas, TX). Biotin-conjugated goat anti-rabbit IgG (H + L) was from Jackson ImmunoResearch (West Grove, PA). Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was from Gene Tex Inc. (Irvine, CA).
Maeda T., Suzuki A., Yuzawa S., Baba Y., Kimura Y, & Kato Y. (2015). Mineral trioxide aggregate induces osteoblastogenesis via Atf6. Bone Reports, 2, 36-43.
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3

Antibody Purchasing and Handling Protocol

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The DATS (purity about 99%) was purchased from LKT Laboratories (St. Paul, MN, USA), dissolved in dimethyl sulfoxide (DMSO; 28 mM stock), and stored at –80°C prior to use. Antibodies against neurofascin (NFASC), ribosomal protein L11 (RPL11), ribosomal protein S14 (RPS14), NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), and NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1) were purchased from Proteintech Group (Rosemont, IL, USA). The N-acetyltransferase domain containing 1 (NATD1) antibody was from Aviva Systems Biology (San Diego, CA, USA). The antibodies against p38 α/β mitogen-activated protein kinase (MAPK), phospho-(Tyr 182)-p38, extracellular-signal-related kinase 1 (ERK1), and phospho-(Tyr 204)-ERK were from Santa Cruz Biotechnology (Dallas, TX, USA). The c-Jun N-terminal kinase 2 (JNK2) antibody not recognizing JNK1 or JNK3 but detecting endogenous JNK2 at a molecular weight of 46 and 54 kDa and phospho-(Thr183/Tyr185)-JNK antibody were from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin antibody was from Sigma-Aldrich (St. Louis, MO, USA) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was from GeneTex (Irvine, CA, USA).
Hahm E.R, & Singh S.V. (2022). Gene Expression Changes by Diallyl Trisulfide Administration in Chemically-induced Mammary Tumors in Rats. Journal of Cancer Prevention, 27(1), 22-30.
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4

Apoptosis Signaling Pathway Analysis

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LLM was purchased from Cayman Chemical Company (Ann Arbor, MI). Chemicals required for cell culture were purchased from Invitrogen-Life Technologies (Carlsbad, CA). The antibodies were purchased from the following vendors: polyclonal anti-Bak and anti-Bax antibodies (for western blotting) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); anti-manganese superoxide dismutase (MnSOD) and anti-active Bak (clone-TC-100 for immunocytochemistry) antibodies were purchased from Calbiochem (Billerica, MA); monoclonal 6A7 antibody specific for detection of active Bax was from BD Biosciences (San Diego, CA); and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was from GeneTex (Irvine, CA). Cell Death Detection ELISAPLUS kit was from Roche Applied Sciences (Indianapolis, IN). MitoSOX Red and MitoTRACKER green were purchased from Invitrogen-Life Technologies. Annexin V-FITC/propidium iodide (PI) Apoptosis detection kit was purchased from BD Biosciences. A kit for detection of active Caspase 9 and anti-cytochrome c antibody were from Abcam (Cambridge, MA).
Sehrawat A., Kim S.H., Hahm E.R., Arlotti J.A., Eiseman J., Shiva S.S., Rigatti L.H, & Singh S.V. (2016). Cancer-Selective Death of Human Breast Cancer Cells by Leelamine Is Mediated by Bax and Bak Activation. Molecular carcinogenesis, 56(2), 337-348.
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5

Withaferin A-Induced Apoptosis Signaling

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Withaferin A (WA, purity > 95%) was purchased from ChromaDex (Irvine, CA) and dissolved in dimethyl sulfoxide (DMSO). Culture media were purchased from MediaTech (Manassas, VA). Fetal bovine serum and antibiotics were purchased from Invitrogen-Life Technologies (Carlsbad, CA). Annexin V/propidium iodide assay kit for apoptosis detection was purchased from BD Biosciences (San Jose, CA), whereas Cell Death Detection ELISAPLUS kit was from Roche Diagnostics (Indianapolis, IN). Antibodies against Pin1 and Cdc25C were purchased from Cell Signaling Technology (Danvers, MA); anti-Cyclin B1 and anti-Cdc2 antibodies were from Santa Cruz Biotechnology (Dallas, TX); anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was from GeneTex (Irvine, CA); and anti-β-Actin and anti-β-Tubulin antibodies were from Sigma-Aldrich (St. Louis, MO). Recombinant human Pin1 protein was purchased from MyBioSource (San Diego, CA). Human Apoptosis Antibody Array was from Abcam (Cambridge, MA).
Samanta S.K., Lee J., Hahm E.R, & Singh S.V. (2018). Peptidyl-prolyl cis/trans isomerase Pin1 regulates withaferin A-mediated cell cycle arrest in human breast cancer cells. Molecular carcinogenesis, 57(7), 936-946.
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6

Cellular Signaling Pathway Modulation

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Dulbecco’s modified Eagle’s medium (DMEM)/F-12 medium, fetal bovine serum (FBS), and TRIzol were from Invitrogen (Carlsbad, CA, USA). The Hybond C membrane and enhanced chemiluminescence (ECL) Western blot detection system were from GE Healthcare Biosciences (Buckinghamshire, UK). The phospho-ERK1/2 (Thr202/Tyr204) (Cat.#4370), phospho-p38 (Thr180/Tyr182) (Cat.#4511), phospho-JNK1/2 (Thr183/Tyr185) (Cat.#4668), and phospho-p65 NF-κB (Ser536) (Cat.#3033) antibodies were from Cell Signaling (Danver, MA, USA). The anti-ZO-1 (Cat.#sc-33725) antibody was from Santa Cruz (Dallas, TX, USA). The anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was from GeneTex (Irvine, CA, USA). The R-7050, U0126, SB202190, SP600125, Bay11-7082, and MMP2/9 inhibitor (2/9i) were from Enzo (Farmingdale, NY, USA). The bicinchoninic acid (BCA) protein assay reagent was from Pierce (Rockford, IL, USA). The tumor necrosis factor-α (TNF-α) was from R&D Systems (Minneapolis, MN, USA). The sophoraflavanone G (SG) was from ChemFaces (Wuhan, China). The enzymes and other chemicals were from Sigma (St. Louis, MO, USA).
Lee T.H., Chen J.L., Tsai M.M., Wu Y.H., Tseng H.C., Cheng L.C., Shanmugam V, & Hsieh H.L. (2023). Protective Effects of Sophoraflavanone G by Inhibiting TNF-α-Induced MMP-9-Mediated Events in Brain Microvascular Endothelial Cells. International Journal of Molecular Sciences, 25(1), 283.
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7

Pharmacological Modulation of Autophagy

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WA (purity > 95%) was purchased from ChromaDex (Irvine, CA), dissolved in dimethyl sulfoxide (DMSO; 20 mM stock), and stored at −80°C. The 3-methyl adenine (3-MA) was from Sigma (St. Louis, MO). Bafilomycin A1 (BafA1) and EUK134 were from Selleckchem (Houston, TX). Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was from GeneTex (Irvine, CA). Antibodies against LC3B, SQSTM1, cleaved poly (ADP-ribose) polymerase (PARP), and cleaved caspase-3 were from Cell Signaling (Danvers, MA). An antibody against GABARAPL1 was from Proteintech (Rosemont, IL). Nonspecific control small interfering RNA (siRNA) and GABARAPL1-targeted siRNA were purchased from Qiagen (Valencia, CA) and Santa Cruz Biotechnology (Dallas, TX), respectively.
Hahm E.R, & Singh S.V. (2020). Cytoprotective autophagy induction by withaferin A in prostate cancer cells involves GABARAPL1. Molecular carcinogenesis, 59(10), 1105-1115.
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8

FoxQ1 Expression in Breast Cancer Cells

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The WA (purity > 95%) was purchased from ChromaDex (Irvine, CA) and dissolved in dimethyl sulfoxide (DMSO). Reagents for cell culture, including fetal bovine serum, cell culture media, and antibiotic mixture were purchased from Life Technologies-Thermo Fisher Scientific (Waltham, MA). An antibody against FoxQ1 was purchased from Proteintech (Rosemont, IL) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was from Genetex (Irvine, CA). An antibody against β-Actin was from Sigma-Aldrich (St. Louis, MO). The MDA-MB-231 and MCF-7 cell lines were purchased from the American Type Culture Collection (Manassas, VA), whereas SUM159 cell line was from Asterand Bioscience (Detroit, MI). Each cell line was last authenticated by us in 2017 by short tandem repeat profiling. Each cell line was cultured according to the supplier’s recommendations. Details of stable transfection of MCF-7 and SUM159 cells with pCMV6 empty vector and the same vector encoding FoxQ1 have been described by us previously (24 (link)).
Kim S.H., Singh K.B., Hahm E.R, & Singh S.V. (2021). The Role of Forkhead Box Q1 Transcription Factor in Anticancer Effects of Withaferin A in Breast Cancer. Cancer prevention research (Philadelphia, Pa.), 14(4), 421-432.
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