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Anti adiponectin antibody

Manufactured by Abcam
Sourced in United Kingdom

Anti-adiponectin antibody is a laboratory reagent used to detect and quantify the presence of the adiponectin protein in biological samples. Adiponectin is an adipokine involved in regulating glucose and lipid metabolism.

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2 protocols using anti adiponectin antibody

1

Citrated FFP from Mouse and Human

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Citrated FFPs of mouse or human origin (designated mFFP or hFFP) were obtained from Innovative Research (Novi, MI) and the Gulf Coast Regional Blood Center (Houston, TX), respectively. For both in vivo and in vitro experiments, FFPs were pooled from three individual donors to minimize donor-to-donor variations. The FFP was thawed and used on the same day (<6 hours), which is defined as day 0 (9 (link)). Evans blue dye was purchased from Sigma-Aldrich (St. Louis, MO). Anti-adiponectin antibody (derived from rabbit, specific for both human and mouse species) and the isotype-matched rabbit IgG were obtained from Abcam (Cambridge, MA). Human or mouse recombinant globular adiponectin (rgAdpn) was obtained from MBL International (Woburn, MA).
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2

Adipogenic Differentiation Assay Protocol

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Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and newborn calf serum (BCS) were purchased from Gibco Life Technologies (Grand Island, NY, USA), Insulin, indomethacin, dexamethasone, 3-isobutyl-1methylxanthine, and Oil Red O solution were purchased from Sigma-Aldrich (St Louis, MO, USA). 3-(4,5)-dimethylthiazo-2-yl-2,5-diphenyltetrazolium bromide (MTT) was purchased from Amresco (Solon, OH, USA). Primers were purchased from Macrogen (Seoul, Korea). Anti-C/EBPα antibody and anti-β-actin antibody were purchased from Cell Signaling Technology (Beverly, MA, USA) and Santa Cruz Biotechnology (Santa Cruz, CA, USA), respectively. Anti-PPARγ antibody and anti-adiponectin antibody were purchased from Abcam (Cambridge, UK).
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