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Lentivirus packaging kit lv500a 1

Manufactured by System Biosciences

The Lentivirus Packaging Kit (LV500A-1) is a laboratory tool designed for the production of lentiviral particles. It contains the necessary components, including plasmids and transfection reagents, to enable the packaging of lentiviral particles in a cell line.

Automatically generated - may contain errors

2 protocols using lentivirus packaging kit lv500a 1

1

Investigating miR-193b and DNMT1 in Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Trypsin, Dulbecco's Modified Eagle Medium (DMEM), MEM vitamins, MEM nonessential amino acids and Penicillin-Streptomycin were purchased from Media Tech (Manassas, VA). TaqMan miRNA assay for hsa-miR-193b was obtained from Applied Biosystems (Foster City, CA). pre-miR-193b, and scrambled pre-miR negative control were from Ambion (Austin, TX), miRCURY LNA anti-miR-193b and scrambled anti-miR negative control were from Exiqon (Vedbæk, Denmark). The lentivirus vector expressing copepod (c) GFP (CD511B-1) and pre-miR-193b, miR-Zip-193b microRNA inhibitor vector, the control vectorsand the lentivirus packaging kit (LV500A-1) were purchased from System Biosciences (Mountain View, CA). The DNMT inhibitor 5-Aza-2′-deoxycytidine (decitabine) was purchased from Sigma-Aldrich, St. Louis, MO (Cat# A3656). The siRNA's used were Dharmacon (Lafayette, CO) SMARTpool siRNAs: DNMT1 siRNA M-004605-01 and siGENOME PLAU siRNA M-006000-02
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2

Investigating miR-193b and DNMT1 in Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Trypsin, Dulbecco's Modified Eagle Medium (DMEM), MEM vitamins, MEM nonessential amino acids and Penicillin-Streptomycin were purchased from Media Tech (Manassas, VA). TaqMan miRNA assay for hsa-miR-193b was obtained from Applied Biosystems (Foster City, CA). pre-miR-193b, and scrambled pre-miR negative control were from Ambion (Austin, TX), miRCURY LNA anti-miR-193b and scrambled anti-miR negative control were from Exiqon (Vedbæk, Denmark). The lentivirus vector expressing copepod (c) GFP (CD511B-1) and pre-miR-193b, miR-Zip-193b microRNA inhibitor vector, the control vectorsand the lentivirus packaging kit (LV500A-1) were purchased from System Biosciences (Mountain View, CA). The DNMT inhibitor 5-Aza-2′-deoxycytidine (decitabine) was purchased from Sigma-Aldrich, St. Louis, MO (Cat# A3656). The siRNA's used were Dharmacon (Lafayette, CO) SMARTpool siRNAs: DNMT1 siRNA M-004605-01 and siGENOME PLAU siRNA M-006000-02
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