HEK-Cre+ cells and the 3×FLAG-mIR cell line were grown out in 24 well plates on glass slides. The HEK-Cre+ cells were transfected with the pPNTlox2 or the pPNTlox2-3×FLAG-mIR plasmid and stained 48 h post transfection. All samples were incubated with the plasma membrane dye, Cell Navigator™ Cell Plasma Membrane Staining Kit (AAT Bioquest). Following plasma membrane staining, cells were fixed with 4% paraformaldehyde, blocked with 2% BSA, and stained with DYKDDDDK FLAG-tagged specific polyclonal Alexa Fluor® 647 conjugated antibody (Cell Signaling Technology). Cells were mounted to glass microscope slides via DAPI Fluoromount-G® (SouthernBiotech) and dried. Image analysis was carried out utilizing a Leica TCS SP5 multiphoton laser scanning confocal microscope (Leica Microsystems) using a 63 × 1.4 NA lens.
Cell navigator cell plasma membrane staining kit
Manufactured by AAT Bioquest
The Cell Navigator™ Cell Plasma Membrane Staining Kit is a fluorescent labeling solution designed to specifically stain the cell plasma membrane. It provides a sensitive and reliable method for visualizing and analyzing cell membrane structures under a fluorescence microscope.
Automatically generated - may contain errors
2 protocols using cell navigator cell plasma membrane staining kit
1
Visualization of FLAG-tagged mIR Expression in HEK-Cre+ Cells
2
Visualizing FLAG-Tagged Protein Expression in Cells
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HEK-Cre+ cells and the 3×FLAG-mIR cell line were grown out in 24 well plates on glass slides. The HEK-Cre+ cells were transfected with the pPNTlox2 or the pPNTlox2-3×FLAG-mIR plasmid and stained 48 h post transfection. All samples were incubated with the plasma membrane dye, Cell Navigator™ Cell Plasma Membrane Staining Kit (AAT Bioquest). Following plasma membrane staining, cells were fixed with 4% paraformaldehyde, blocked with 2% BSA, and stained with DYKDDDDK FLAG-tagged specific polyclonal Alexa Fluor® 647 conjugated antibody (Cell Signaling Technology). Cells were mounted to glass microscope slides via DAPI Fluoromount-G® (SouthernBiotech) and dried. Image analysis was carried out utilizing a Leica TCS SP5 multiphoton laser scanning confocal microscope (Leica Microsystems) using a 63 × 1.4 NA lens.
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