Sc 36868

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-36868 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for use in scientific research and laboratory settings. The core function of this product is to provide a specific technical capability, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

20 protocols using sc 36868

Eosinophil siRNA Transfection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Freshly isolated eosinophils were resuspended (10⁶ cells/ml) in antibiotic-free siRNA transfection medium (sc-36868, Santa Cruz) without FBS. One ml aliquots of eosinophils in transfection medium were added to each well of a 12-well culture plate. An aliquot of 3.6 µl of PP5 siRNA was added to 40 µl of siRNA Transfection Medium (sc-36868, Santa Cruz) and kept at RT for 5 min. In a separate tube, 2.4 µl of siRNA Transfection Reagent (sc-29528) was mixed with 40 µl of siRNA Transfection Medium and then kept at RT for 5 min. Subsequently, the contents from both tubes were mixed and incubated at RT for 20 min to form an siRNA complex. After 20 min of incubation, 0.32 ml of siRNA Transfection Medium was added to each tube containing the siRNA transfection complex. Subsequently, cells were resuspended in 0.4 ml of resulting siRNA/siRNA transfection complex and incubated for 8 h at 37°C. After incubation, cells were washed and resuspended in 1 ml of RPMI 1640 containing 10% of FBS followed by an additional incubation of 36 h. Transfection efficiency was evaluated by Western blotting for each experiment performed on eosinophil viability. Control siRNA (sc-370007) was a scrambled sequence that did not cause degradation of any known cellular mRNA.

Pazdrak K., Straub C., Maroto R., Stafford S., White W.I., Calhoun W.J, & Kurosky A. (2016). Cytokine-Induced Glucocorticoid Resistance from Eosinophil Activation: Protein Phosphatase 5 Modulation of Glucocorticoid Receptor Phosphorylation and Signaling. Journal of immunology (Baltimore, Md. : 1950), 197(10), 3782-3791.

+ Open protocol

+ Expand

Silencing Top2α via siRNA Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols

A Top2α siRNA pool (h) (SC-36695), control nonsense siRNA (SC-37007), transfection media (SC-36868) and transfection reagent (SC29528) were commercially obtained from Santa Cruz Biotechnology. Transfection reactions were carried out according to the manufacturer’s protocol with slight modification. Briefly, cells were cultured in antibiotic free media one day before transfection. Cells were transfected with siRNA for 6 hr at 37 °C in a 5% CO₂ incubator by transfection reagent. Treatment with YM155 or doxorubicin was performed after culturing in regular culture media for another 24 hr. Simple western was performed to confirm that the knockdown was successful in reducing protein expression (Fig. S2).

Mackay R.P., Weinberger P.M., Copland J.A., Mahdavian E, & Xu Q. (2022). YM155 induces DNA damage and cell death in anaplastic thyroid cancer cells by inhibiting DNA topoisomerase IIα at the ATP binding site. Molecular cancer therapeutics, 21(6), 925-935.

+ Open protocol

+ Expand

Efficient Knockdown of ATF2 in JB6 P+ Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

JB6 P+ cells were seeded (2 x 10⁵ cells/well) in six-well tissue culture plates, and incubated at 37°C in a 5% CO2 incubator until becoming 70–80% confluent. For each transfection, 2 μl of ATF2 siRNA duplex (sc-29756, Santa Cruz Biotechnology, Santa Cruz, CA) was diluted into 100 μl of siRNA transfection medium (sc-36868, Santa Cruz Biotechnology). In a separate tube, 2 μl of transfection reagent (sc-29528, Santa Cruz Biotechnology) was diluted into 100 μl of siRNA transfection medium. The dilutions were mixed gently together and incubated for 30 min at room temperature. Fluorescein conjugated control siRNA (sc-36869, Santa Cruz Biotechnology) was used to monitor the transfection efficiency, which was approximately 70%.

Li W., Zhang C., Ren A., Li T., Jin R., Li G., Gu X., Shi R, & Zhao Y. (2015). Shikonin Suppresses Skin Carcinogenesis via Inhibiting Cell Proliferation. PLoS ONE, 10(5), e0126459.

+ Open protocol

+ Expand

Silencing p66Shc and Nrf2 in K562 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

K562 cells (1 × 10⁷) were electroporated with 200 pmol of siRNA by Gene Pulser X-Cell in 100 μl of siRNA transfection medium (sc-36868; Santa Cruz Biotechnology). After electroporation, cells were incubated at 37°C for 10 min, transferred into fresh culture medium, and incubated at 37°C for 24–48 h. p66Shc-1 siRNA: sense, 5′-CGAUAGUCCCACUACCCUGUU-3′, and antisense, 5′-CAGGGUAGUGGGACUAUCGUU-3′ (custom order; Thermo Scientific); p66Shc-2 siRNA: sense: 5′-GGAUGAGCAACCUGAGGCUTT-3′, and antisense, 5′-AGCCUCAGGUUGCUCAUCCGG-3′ (custom order; Qiagen, Valencia, CA); Nrf2 siRNA-1: sense, 5′-UGGAGUAAGUCGAGAAGUAUU-3′, and antisense, 5′-PUACUUCUCGACUUACUCCAUU-3′ (J-003755-11; Dharmacon, Lafayette, CO); Nrf2 siRNA-2: sense, 5′-CAUUGAUGUUUCUGAUCUATT-3′, and antisense, 5′-UAGAUCAGAAACAUCAAUGGG-3′ (Sl03246950; Qiagen); and nontargeting siRNA (si Control; 1027281; Qiagen).

Miyazawa M, & Tsuji Y. (2014). Evidence for a novel antioxidant function and isoform-specific regulation of the human p66Shc gene. Molecular Biology of the Cell, 25(13), 2116-2127.

+ Open protocol

+ Expand

Nrf2 Knockdown in HLECs for Oxidative Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

The siRNA targeting the Nrf2(sc-37030) was adopted for knocking down Nrf2 levels. We analyzed transfection efficiency using fluorescein-labeled control siRNA(sc-36869). HLECs were first seeded into a 6-well plate, followed by incubation under 5% CO₂ and 37°C conditions till then reached 70–80% confluence. Then, in terms of each transfection, we diluted Nrf2 or control siRNA (2 μL) and transfection reagent (2 μL, sc-29528) in the siRNA transfection medium (100 μL, sc-36868; Santa Cruz, CA, USA), separately. Then, we combined dilutions to incubate under ambient temperature for 0.5 h. After that, cells were transfected for 12 h and subsequently treated with 50 μg/mL KGF-2 for 2 h before the treatment with 100 μM H₂O₂ for 12 h.

Liu S., Jin Z., Xia R., Zheng Z., Zha Y., Wang Q., Wan X., Yang H, & Cai J. (2022). Protection of Human Lens Epithelial Cells from Oxidative Stress Damage and Cell Apoptosis by KGF-2 through the Akt/Nrf2/HO-1 Pathway. Oxidative Medicine and Cellular Longevity, 2022, 6933812.

+ Open protocol

+ Expand

Silencing HDGF Gene in LX2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Inhibition of HDGF in RLW cells was achieved by HDGF siRNA transfection following the manufacturer’s instructions. Four hours before the siRNA application, FBS-rich medium (DMEM, 2% FBS, and 1% Penicillin streptomycin) was replaced with FBS-deficient media (DMEM and 1% Penicillin streptomycin) in the LX2 culture system. Then the cells were incubated with siRNA in the transfection media (sc-36868, Santa Cruz, Dallas, TX, USA) for 5–6 h. The efficiency of siRNA transfection was evaluated using scrambled siRNA-FITC Conjugate-A (sc-36869, Santa Cruz, Dallas, TX, USA), and approximately 93.75% of siRNA FITC-positive cells was detected by immunostaining (Figure 9). After successful transfection of HDGF siRNA, the same cells were pretreated with AGS for 24 h, then HIV overnight and AGS for 48 h. HDGF-siRNA-transfected cells could not be incubated for more than 48 h due to the chance of reactivating the silenced HDGF gene. Control siRNA (sc-37007, Santa Cruz, Dallas, TX, USA) was included in the experiment as a negative control.

New-Aaron M., Koganti S.S., Ganesan M., Kanika S., Kumar V., Wang W., Makarov E., Kharbanda K.K., Poluektova L.Y, & Osna N.A. (2023). Hepatocyte-Specific Triggering of Hepatic Stellate Cell Profibrotic Activation by Apoptotic Bodies: The Role of Hepatoma-Derived Growth Factor, HIV, and Ethanol. International Journal of Molecular Sciences, 24(6), 5346.

+ Open protocol

+ Expand

Cx43 siRNA-Mediated Protein Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were transfected with 8 μl Cx43 short interfering RNA (siRNA; siCx43; SC6008, Santa Cruz Biotechnology, Dallas, TX, USA) for 6 h at 37 °C in a 5% CO₂ incubator. Then, cells were incubated with DMEM containing 20% fetal calf serum for an additional 48 h. For this experiment, transfection reagent (SC29528, Santa Cruz Biotechnology), transfection medium (SC36868, Santa Cruz Biotechnology), and FITC-labeled control siRNA (SC37007, Santa Cruz Biotechnology) were used. The proteins generated were quantified via western blot.

Li L., Liu H., Xu C., Deng M., Song M., Yu X., Xu S, & Zhao X. (2017). VEGF promotes endothelial progenitor cell differentiation and vascular repair through connexin 43. Stem Cell Research & Therapy, 8, 237.

+ Open protocol

+ Expand

siRNA Transfection Protocol for Caki-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Caki-1 cells (50%–70% confluence) were transfected with siRNA duplexes using the protocol supplied with the siRNA transfection reagent sc-29528 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Briefly, 5 μL of siRNA duplex (i.e., 0.6 μg siRNA) was diluted into 100 μL siRNA transfection medium: sc-36868 (Santa Cruz Biotechnology) without serum and antibiotics for about 20 min to form a lipid–siRNA complex. Transfection was initiated by adding the lipid–siRNA complex to six-well plates. Cells were incubated for 6 h at 37 °C in a CO₂ incubator. The final concentration of siRNA was 30 nM. Then, 1 mL of normal growth medium containing two times the normal serum and antibiotics concentration (2× normal growth medium) was added to the cells, without removing the transfection mixture, and incubated for an additional 24 h. Finally, the medium was aspirated and replaced with fresh 1× normal growth medium.

Antonaci G., Cossa L.G., Muscella A., Vetrugno C., De Pascali S.A., Fanizzi F.P, & Marsigliante S. (2019). [Pt(O,O′-acac)(γ-acac)(DMS)] Induces Autophagy in Caki-1 Renal Cancer Cells. Biomolecules, 9(3), 92.

+ Open protocol

+ Expand

Glucose-Induced IRE1α Knockdown in RSC96 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

RSC96 cells plated at a density of 2 × 10⁵cells/well in 6-well plate were allowed to adhere overnight, and then transfected with IRE1α siRNA according to the manufacturer’s instructions. Briefly, for each transfection, 100 pmols of siRNA and 6 µl transfection reagent were added to 100 µl siRNA transfection medium (sc-36868, Santa Cruz), gently mixed, incubated for 25 minutes at room temperature, then 1.0 ml siRNA transfection medium containing 200 µl siRNA transfection reagent mixture was added to the well. Cells were incubated 8 hours at 37 °C in a CO₂ incubator, 1 ml normal growth medium containing 2 times the normal serum and antibiotics concentration (2× normal growth medium) was added without removing the transfection mixture. Cells were incubated for an additional 16 hours and the medium was aspirated and cultured in 25 mM or 150 mM glucose growth medium for 24 hours and 48 hours, respectively.
Cells were treated with 25 mM glucose, 150 mM glucose, 25 mM glucose IRE1α siRNA transfected (25 mM glucose + IRE1α siRNA) and 150 mM glucose IRE1α siRNA transfected (150 mM glucose + IRE1α siRNA). Cells in 25 mM glucose, 150 mM glucose groups were treated with siRNA transfection reagent alone (Supplementary Fig. 1).

Yao W., Yang X., Zhu J., Gao B., Shi H, & Xu L. (2018). IRE1α siRNA relieves endoplasmic reticulum stress-induced apoptosis and alleviates diabetic peripheral neuropathy in vivo and in vitro. Scientific Reports, 8, 2579.

+ Open protocol

+ Expand

Investigating Nrf2 Regulation in K562 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

K562 cells electroporated with 100 pmol of non-targeting siRNA (siControl;
D-001210-01) or siNrf2 (J-003755-11) from Thermo Fisher Scientific in 100 μl of
siRNA transfection medium (sc-36868; Santa Cruz Biotechnology) were incubated at room
temperature for 10 min, transferred into cell culture dishes with growth medium, and
incubated at 37°C for 48 h. Cells were then treated with cobalt chloride and
analyzed expression of mRNAs in RT-qPCR.

Huang B.W., Miyazawa M, & Tsuji Y. (2014). Distinct Regulatory Mechanisms of the Human Ferritin Gene by Hypoxia and Hypoxia Mimetic Cobalt Chloride at the Transcriptional and Post-transcriptional Levels. Cellular signalling, 26(12), 2702-2709.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!