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Theaflavin

Manufactured by Merck Group
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Sourced in United States, China

Theaflavin is a compound found in black tea. It is a polyphenol that has been studied for its potential antioxidant and anti-inflammatory properties. Theaflavin can be used as a reference standard or analytical tool in laboratory settings.

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Lab products found in correlation

Epigallocatechin gallate (egcg), by Merck Group (4 mentions) Catechin, by Merck Group (3 mentions) Gallic acid, by Merck Group (3 mentions) Catechin gallate, by Merck Group (2 mentions) Bile salt, by Merck Group (2 mentions) Streptozotocin (stz), by Merck Group (2 mentions) Cholesterol, by Merck Group (2 mentions) Egg yolk power, by Merck Group (2 mentions) Theaflavin 3 gallate, by Merck Group (1 mentions) Gallocatechin gc, by Merck Group (1 mentions) L theanine, by Merck Group (1 mentions) Epicatechin gallate ecg, by Merck Group (1 mentions) Gallocatechin gallate gcg, by Merck Group (1 mentions) Epigallocatechin egc, by Merck Group (1 mentions) Citric acid, by Merck Group (1 mentions) Caffeic acid, by Merck Group (1 mentions) Ellagic acid, by Merck Group (1 mentions) Protocatechuic acid, by Merck Group (1 mentions) Folin cioc lteu reagent, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions)

Close spelling variants of this product

Theaflavin-3,3’-digallate (3 citations) Theaflavin monogallate (2 citations) Theaflavin-3-monogallate (1 citations) Theaflavin-3-gallate (1 citations)

7 protocols using theaflavin

1

Tea Catechins and Theaflavins Extraction

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The catechins including (−)-gallocatechin (GC), (−)-epigallocatechin (EGC), (+)-catechin (C), (−)-epicatechin (EC), (−)-epigallocatechin gallate (EGCG), (−)-gallocatechin gallate (GCG), (−)-epicatechin gallate (ECG), (−)-catechin gallate (CG), caffeine, l-theanine and eighteen amino acid standards (kit) were purchased from Sigma (St. Louis, MO, USA). Four theaflavin monomers including theaflavin (TF1, 95%), theaflavin-3-gallate (TF2A, 92%), theaflavin-3′-gallate (TF2B, 90%) and theaflavin-3, 3′-digallate (TF3, 90%) were prepared according to the method developed in our lab, and their purities were determined by high-performance liquid chromatography-ultraviolet-mass spectrum (HPLC-UV-MS), with the standard samples from sigma company.12 (link) Methanol and acetonitrile of HPLC grade were purchased from Tianjin Shield Company (Tianjin, China). Other reagents of analytical reagent grade were purchased from National Medicine Chemical Reagent Co., Ltd. (Shanghai, China).
Liu X., Liu Y., Li P., Yang J., Wang F., Kim E., Wu Y., He P., Li B, & Tu Y. (2021). Chemical characterization of Wuyi rock tea with different roasting degrees and their discrimination based on volatile profiles. RSC Advances, 11(20), 12074-12085.
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2

HPLC Analysis of Bioactive Compounds

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The HPLC analytical-grade hexane, acetone, acetonitrile, ethyl acetate, methanol and analytical grade 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), hydrochloric acid, citric acid, sodium hydroxide, sodium chloride, aluminum chloride, ethanol, Folin–Ciocâlteu reagent, gallic acid, inulin were purchased by Sigma Aldrich (Darmstadt, Germany). For cromatographic analysis the following reagents were used: HCl ACS reagent (37%), acetic acid, methanol, ethyl acetate, acetonitrile, theaflavin, cafestol, procyanidin A1, procyanidin B1, (−)-epigallocatechin, catechin, caffeine, caffeic acid, ellagic acid, gallic acid, protocatechuic acid, trans-cinnamic acid, quercetin 3-glucoside, quercetin 3-D-galactoside, quercetin 3-β-D-glucoside, naringin, hesperidin, myricetin, apigenin, kaempferol, luteolin, and isorhamnetin (HPLC-grade), purchased from Sigma-Aldrich (Darmstadt, Germany). Other reagents such as sodium bicarbonate were purchased from Honeywell, Fluka (Seelze, Germany). The Lo. bifermentans MIUG BL 16 strain was from Microorganism Collection of Dunarea de Jos University (acronym MIUG, Galati, Romania). de Man, Rogosa and Sharpe agar (MRS agar) was purchased from Merck (Darmstadt, Germany).
Vlad C.C., Păcularu-Burada B., Vasile A.M., Milea Ș.A., Bahrim G.E., Râpeanu G, & Stănciuc N. (2022). Upgrading the Functional Potential of Apple Pomace in Value-Added Ingredients with Probiotics. Antioxidants, 11(10), 2028.
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3

Theaflavin and Streptozotocin in High-Fat Diet

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Theaflavin and streptozotocin, high-fat diet components such as cholesterol, bile salt, egg yolk power, and lard were obtained from Sigma Chemical Company (St. Louis, MO, USA), Sisco Research Laboratories Pvt. Ltd. (Mumbai, India), Central Drug House Pvt. Ltd. (New Delhi, India), SKM Egg Products Export (India) Limited (Erode, Tamil Nadu, India), and lard was obtained from local market in Chennai. All other chemicals used were of analytical grade.
Gothandam K., Ganesan V.S., Ayyasamy T, & Ramalingam S. (2019). Protective effect of theaflavin on glycoprotein components and TCA cycle enzymes in high-fat diet and streptozotocin-induced diabetic rats. Journal of Basic & Applied Zoology, 80(1), 43.
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4

Investigating Dietary Modulators of Metabolism

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Theaflavin, streptozotocin and high fat diet components such as cholesterol, bile salt, egg yolk power and lard were obtained from Sigma Chemical Company (St. Louis, MO, USA), Sisco Research Laboratories Pvt. Ltd., Mumbai, India, Central Drug House Pvt. Ltd., New Delhi, India, SKM Egg Products Export (India) Limited, Erode, Tamil Nadu, India and lard was obtained from local market in Chennai. All other chemicals used were of analytical grade.
Gothandam K., Ganesan V.S., Ayyasamy T, & Ramalingam S. (2019). Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats. Redox Report : Communications in Free Radical Research, 24(1), 41-50.
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5

Polyphenol Compound Procurement

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Gallic acid, catechin, catechin gallate, epicatechin, epicatechin gallate, epigallocatechin gallate (EGCG), theaflavin, theaflavin monogallate, theaflavin digallate and tannic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Ferreira N., Pereira-Henriques A, & Almeida M.R. (2015). Transthyretin chemical chaperoning by flavonoids: Structure–activity insights towards the design of potent amyloidosis inhibitors. Biochemistry and Biophysics Reports, 3, 123-133.
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6

HPLC Analysis of Polyphenol Composition in Brewed Tea

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The GT and BT polyphenol composition of brewed tea (one bag in 240 mL of boiling water for 5 min) was determined by HPLC with CoulArray electrochemical detection (ESA, Chelmsford, MA) as previously described (GT: mg/L: 855.0±11.8 total phenolics, 396.8 ± 28.3 EGCG, 202.3 ± 10.9 EGC, 52.1 ± 4.7 EC, 62.1 ± 4.6 ECG, 11.8±2.3 gallic acid and 0 THE and BT: mg/L: 661.5±5.8 total phenolics, 28.1±0.8 EGCG, 5.6±0.5 EGC, 8.4±0.2 EC, 15.5±0.3 ECG, 242.1±3.6 gallic acid and 24.6±2.5 THE) [17 ]. All HPLC grade solvents were purchased from Fisher Scientific (Pittsburgh, PA). EGCG, EC, ECG, EGC and black tea extract (mixture of theaflavin, theaflavin-3-monogallate, theaflavin-3’-monogallate and theaflavin-3,3’-digallate) were purchased from Sigma-Aldrich (St.Louis, MO), 4'-O-methyl EGC (4'-MeEGC) and 4"-O-methyl EGCG (4"-MeEGCG) were purchased from Nacalai USA Inc. (San Diego, CA) and theaflavin-3,3’-digallate and theaflavin-3-gallate from Wako Chemicals USA (Richmond VI).
Henning S.M., Wang P., Said J.W., Huang M., Grogan T., Elashoff D., Carpenter C.L., Heber D, & Aronson W.J. (2014). Randomized Clinical Trial of Brewed Green and Black Tea in Men with Prostate Cancer Prior to Prostatectomy. The Prostate, 75(5), 550-559.
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7

Evaluating Polyphenol Effects on Fish

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Experiments were performed on swordfish, grouper, tuna and salmon filets obtained from fish markets in Montreal. These species were selected to reflect fish readily available to Canadian consumers year-round. Blueberries, coffee (Nescafé, Maxwell) and green and black teas of various brands (Twinnings, Stash, Green Sail, Salada) were purchased in Montreal supermarkets as were corn oil (Mazola) used for cooking treatments, and cornstarch (Ideal), used as a nonpolyphenol control. Pure polyphenols (gallic acid (>97.5%), catechin >98%), epigallocatechin gallate (>80%), theaflavin (>80%), rutin (>94%)) were obtained from Sigma-Aldrich.
Girard C., Charette T., Leclerc M., Shapiro B.J, & Amyot M. (2018). Cooking and co-ingested polyphenols reduce in vitro methylmercury bioaccessibility from fish and may alter exposure in humans. The Science of the total environment, 616-617.
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