Copper palladium

Copper/palladium transmission
electron microscopy (TEM) grids (Agar Scientific, UK) were coated
in-house to yield a thin film of amorphous carbon and were subjected
to a glow discharge for 20 s. An aqueous droplet of a copolymer dispersion
(7.0 μL, 0.1% w/w) was placed on freshly treated grids for 1
min and then carefully blotted with filter paper to remove excess
solution. An aqueous droplet of uranyl formate solution (5.0 μL,
0.75% w/w) was placed on each sample-loaded grid for 1 min and then
blotted with filter paper to remove excess stain. This negative staining
protocol was required to ensure sufficient electron contrast. Each
grid was then carefully dried using a vacuum hose. Imaging was performed
at 80 kV using an FEI Tecnai Spirit 2 microscope fitted with an Orius
SC1000B camera. Mean nanoparticle diameters were estimated by digital
image analysis using ImageJ software.

Copper/palladium transmission
electron microscopy (TEM) grids (Agar Scientific, UK) were coated
in-house with a thin film of amorphous carbon. Grids were then subjected
to a glow discharge for 30 s to create a hydrophilic surface. Each
0.1%
w/w aqueous diblock copolymer dispersion was deposited as a 5.0 μL
droplet onto a freshly treated grid for 1 min and then blotted with
filter paper to remove excess solution. To stain the deposited nanoparticles,
uranyl formate (5.0 μL of a 0.75% w/w aqueous solution) was
placed on the sample-loaded grid for 20 s and then carefully blotted
to remove excess stain. Each grid was then dried using a vacuum hose.
Imaging was performed using a FEI Tecnai Spirit 2 instrument operating
at 80 kV and equipped with an Orius SC1000B camera.