Rat liver, skeletal muscle, small intestines, and skin tissues were fixed in 4% paraformaldehyde and embedded in paraffin blocks. Blocks were sectioned at 3 μm, and sections were stained with hematoxylin and eosin (H&E). Morphological analyses were conducted using the Leica Qwin image analyzer system equipped with a Leica DMLS microscopy.
Qwin image analyzer system
Manufactured by Leica
Sourced in United Kingdom
The Qwin image analyzer system is a versatile and reliable tool designed for microscope-based image analysis. It offers precise quantification of various microscopic samples, providing users with detailed measurements and statistical data. The core function of the Qwin system is to capture, process, and analyze high-quality digital images, enabling users to extract valuable information from their microscopic observations.
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Lab products found in correlation
4 protocols using qwin image analyzer system
1
Tissue Histological Analysis Pipeline
2
Histological Scoring of Colon and Liver
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Tissues of colon and liver were fixed in 4% paraformaldehyde, embedded in paraffin, cut into 3 μm sections, and stained with hematoxylin and eosin (H&E). Morphological assessments of stained slides were performed with the Leica Qwin image analyzer system. Histological scoring was blindly performed by two researchers using the degree of surface epithelial loss, crypt destruction and inflammatory cell infiltration into the mucosa, scoring from 0 to 12 [14 (link)].
3
Histological Examination of Frozen Liver
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Optimal cutting temperature (OCT)-frozen liver samples were cut at 5 μm (CM3050S, Leica Microsystems, Nussloch, Germany). Liver slides were stained with hematoxylin and eosin (H&E) for routine histological examination. Morphological analyses of stained slides were performed using the Leica Qwin image analyzer system with a Leica DMLS microscope.
4
Histological Evaluation of Liver Tissue
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Specimens of liver tissue were fixed in 10% neutral buffered formalin and then embedded in paraffin. After being deparaffinized, 5-μm-thick slices were processed for hematoxylin & eosin (H&E) staining and then examined under the light microscope (Primo star, ZEISS, China) for assessment of the general liver architecture. The level of tissue damage in H&E-stained liver sections was quantified, as previously reported [21 ]. The area percentage of hepatic tissue damage manifested as hepatocyte vacuolation and mononuclear cellular infiltration as well as central and portal veins dilatation and congestion, was analyzed in six randomly chosen microscopic fields of H&E-stained slides using Leica Qwin image analyzer system (Cambridge, UK).
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