The levels of BDNF (ab213899; Abcam Inc, Toronto, Canada) and tumour necrosis factor α (TNF-α) (ab46070; Abcam Inc, Toronto, Canada) were measured by commercially available sandwich based-ELISA kits, and procedure was adapted as the instructions of these kits. The levels of reduced glutathione levels were quantified by fluorometric assay kit (ab235670; Abcam Inc, Toronto, Canada). It was based on the formation of fluorescent product as a result of a reaction between reduced glutathione and a fluorophore. The intensity of fluorescence was measured at Excitation/Emission=535/587 nm, and the intensity was directly proportional to the amount of reduced glutathione in the sample.
Ab235670
Manufactured by Abcam
Sourced in Canada
Ab235670 is a laboratory equipment product offered by Abcam. It is designed for use in scientific research and experiments. The core function of this product is to [CORE FUNCTION DESCRIPTION]. No further details or interpretations are provided.
Automatically generated - may contain errors
3 protocols using ab235670
1
Quantifying Neuroimmune Biomarkers in Samples
2
Measuring Oxidative Stress and Inflammation in Mouse Brain
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Brain tissues from mice sacrificed at the indicated end point were snap-frozen in liquid nitrogen and lysed (RIPA buffer, Thermo Fisher Scientific, Seoul, Korea) before use. Homogenate was centrifuged at 15000 rpm for 30 min at 4 °C. The upper layer was transferred into new containers and subjected to protein assay (Bradford method, Life Science, CA). A total 400 μg of proteins were used in each individual target.
Analyses for Malondialdehyde (MDA, ab118970) and reduced glutathione (GSH, ab235670) from Abcam Company (Cambridge, UK) in tissues were performed according to manufacturer’s manuals.
Enzyme-linked immunosorbent assays (ELSIA) kits to detect TNF-α, IL-6 and IL-1β were purchased from R&D Systems (Minneapolis, MN) and explored according to the manufacture’s protocols. Signals were detected by Tecan plate reader (Männedorf, Zürich, Switzerland).
Antibody arrays kits to detect apoptosis and NF-κB activation purchased from R&D Systems (Minneapolis, MN) and cytokine secretion purchased from RayBiotech (Norcross, GA), were used according to the manufacture’s protocols. Brain lysates (200-500 μg) of mice were obtained from snap-frozen samples in liquid nitrogen and applied to array tests. The signals were detected with Odyssey-LC chemiluminsescent imaging system (LI-COR, Lincoln, NE). Signals were averaged and expressed as described in figure legend.
Analyses for Malondialdehyde (MDA, ab118970) and reduced glutathione (GSH, ab235670) from Abcam Company (Cambridge, UK) in tissues were performed according to manufacturer’s manuals.
Enzyme-linked immunosorbent assays (ELSIA) kits to detect TNF-α, IL-6 and IL-1β were purchased from R&D Systems (Minneapolis, MN) and explored according to the manufacture’s protocols. Signals were detected by Tecan plate reader (Männedorf, Zürich, Switzerland).
Antibody arrays kits to detect apoptosis and NF-κB activation purchased from R&D Systems (Minneapolis, MN) and cytokine secretion purchased from RayBiotech (Norcross, GA), were used according to the manufacture’s protocols. Brain lysates (200-500 μg) of mice were obtained from snap-frozen samples in liquid nitrogen and applied to array tests. The signals were detected with Odyssey-LC chemiluminsescent imaging system (LI-COR, Lincoln, NE). Signals were averaged and expressed as described in figure legend.
3
Glutathione Quantification Assay
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Reduced glutathione (GSH) levels were measured with a commercially available kit (Abcam, ab235670) per vendor instructions.
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