Metabolites were extracted from plasma samples and analyzed by LC-TOF-MS.
An Agilent HPLC 1200 system (Agilent Corporation, Santa Clara, CA, USA) was used with chromatographic separations performed on a 4.6 × 150 mm, 5 μm Agilent ZORBAX Eclipse XDB-C18 chromatography column. Mass spectral data were acquired using an Agilent model 6220 MSD TOF mass spectrometer equipped with a dual sprayer electrospray ionization source (Agilent Corporation). Agilent API-TOF Reference Mass Solution Kit was used to obtain accurate mass time-of-flight data in both positive and negative mode operation. During metabolite profiling, both plot and centroid data were acquired for each sample from 50 to 1000 Da over a 25 min analysis time. Data generated by LC-TOF-MS were semiquantitative data and are expressed in peak intensity. For details, seeSupplementary Experimental Procedures .
An Agilent HPLC 1200 system (Agilent Corporation, Santa Clara, CA, USA) was used with chromatographic separations performed on a 4.6 × 150 mm, 5 μm Agilent ZORBAX Eclipse XDB-C18 chromatography column. Mass spectral data were acquired using an Agilent model 6220 MSD TOF mass spectrometer equipped with a dual sprayer electrospray ionization source (Agilent Corporation). Agilent API-TOF Reference Mass Solution Kit was used to obtain accurate mass time-of-flight data in both positive and negative mode operation. During metabolite profiling, both plot and centroid data were acquired for each sample from 50 to 1000 Da over a 25 min analysis time. Data generated by LC-TOF-MS were semiquantitative data and are expressed in peak intensity. For details, see