Log In Sign up
The largest database of trusted experimental protocols

Wh0152110m1

Manufactured by Abcam
Visit Supplier

WH0152110M1 is a lab equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Hrp conjugated anti mouse igg, by Cell Signaling Technology (2 mentions) Mouse anti acetylated α tubulin, by Merck Group (2 mentions) Hrp conjugated anti rabbit igg, by Cell Signaling Technology (2 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (2 mentions) Mouse anti flag m2, by Merck Group (2 mentions) Rabbit anti raptor, by Merck Group (2 mentions) Hpa038941, by Merck Group (2 mentions)

2 protocols using wh0152110m1

1

Immunoblotting Analysis of NEK10 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were prepared in lysis buffer containing 1% Triton X-100, 10 mM β-glycerol phosphate, 10 mM pyrophosphate, 40 mM HEPES pH 7.4, 2.5 mM MgCl2, and one mini tablet of EDTA-free protease inhibitor (Roche) per 10mL. Lysates were subjected to SDS-PAGE electrophoresis and transferred to PVDF membranes before immunoblotting with the indicated antibodies. Primary antibodies and working dilutions were as follows: rabbit anti-NEK10 (Sigma HPA038941, lot R35857, 1:1000), mouse anti-NEK10 (Sigma WH0152110M1, lot 09058–1C9, 1:1000), rabbit anti-GAPDH (Abcam ab9485, 1:2500), mouse anti-FLAG M2 (Sigma F1804, lot SLBS3530V, 1:1000), rabbit anti-Raptor (Millipore 09–217, lot 3236353, 1:1000), mouse anti-β-actin (Santa Cruz sc-47778, lot K1718, 1:1000), mouse anti-acetylated α-tubulin (Sigma T7451, 1:1000). Secondary antibodies and dilutions were as follows: HRP-conjugated anti-rabbit IgG (Cell Signaling Technologies #7074, 1:3000), HRP-conjugated anti-mouse IgG (Cell Signaling Technologies #7076, 1:3000).
Chivukula R.R., Montoro D.T., Leung H.M., Yang J., Shamseldin H.E., Taylor M.S., Dougherty G.W., Zariwala M.A., Carson J., Daniels L.A., Sears P., Black K.E., Hariri L.P., Almogarri I., Frenkel E.M., Vinarsky V., Omran H., Knowles M.R., Tearney G.J., Alkuraya F.S, & Sabatini D.M. (2020). A human ciliopathy reveals essential functions for NEK10 in airway mucociliary clearance. Nature medicine, 26(2), 244-251.
+ Open protocol
+ Expand
2

Immunoblotting Analysis of NEK10 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were prepared in lysis buffer containing 1% Triton X-100, 10 mM β-glycerol phosphate, 10 mM pyrophosphate, 40 mM HEPES pH 7.4, 2.5 mM MgCl2, and one mini tablet of EDTA-free protease inhibitor (Roche) per 10mL. Lysates were subjected to SDS-PAGE electrophoresis and transferred to PVDF membranes before immunoblotting with the indicated antibodies. Primary antibodies and working dilutions were as follows: rabbit anti-NEK10 (Sigma HPA038941, lot R35857, 1:1000), mouse anti-NEK10 (Sigma WH0152110M1, lot 09058–1C9, 1:1000), rabbit anti-GAPDH (Abcam ab9485, 1:2500), mouse anti-FLAG M2 (Sigma F1804, lot SLBS3530V, 1:1000), rabbit anti-Raptor (Millipore 09–217, lot 3236353, 1:1000), mouse anti-β-actin (Santa Cruz sc-47778, lot K1718, 1:1000), mouse anti-acetylated α-tubulin (Sigma T7451, 1:1000). Secondary antibodies and dilutions were as follows: HRP-conjugated anti-rabbit IgG (Cell Signaling Technologies #7074, 1:3000), HRP-conjugated anti-mouse IgG (Cell Signaling Technologies #7076, 1:3000).
Chivukula R.R., Montoro D.T., Leung H.M., Yang J., Shamseldin H.E., Taylor M.S., Dougherty G.W., Zariwala M.A., Carson J., Daniels L.A., Sears P., Black K.E., Hariri L.P., Almogarri I., Frenkel E.M., Vinarsky V., Omran H., Knowles M.R., Tearney G.J., Alkuraya F.S, & Sabatini D.M. (2020). A human ciliopathy reveals essential functions for NEK10 in airway mucociliary clearance. Nature medicine, 26(2), 244-251.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!