Microglia were isolated from the hippocampus as described above and blocked with rat- anti CD16/CD32 Fc antibodies 1:100 (Cat# 553142, BD Biosciences) and then stained with rat-anti CD11b-PeCy7 1:100 (Cat# 25-0112-82, ebiosciences), rat-anti CD45-PeCPCy5.5 antibodies 1:100 (Cat# 45-0451-82, ebiosciences), APC-rat anti Trem2 antibodies 1:10 (Cat# FAB17291A R & D systems), or APC-mouse anti CX3CR1 antibodies 1:100 (Cat# 149008) for 25 min on ice. Labeled cells were washed with 3 ml of cold FACS buffer, spun as above, resuspended in 300ul of cold FACS buffer and assessed for antigen expression using a BD LSR fortessa FACS (BD Biosciences). Data were analyzed using FlowJo software (Tree Star) and gating for each antibody was determined with isotype-stained control.
Apc rat anti trem2 antibodies
Manufactured by R&D Systems
The APC-rat anti Trem2 antibodies are a laboratory tool used to detect the presence and expression levels of the Trem2 protein in rat samples. The antibodies are conjugated to the APC fluorescent dye, allowing for the identification and quantification of Trem2-positive cells through flow cytometry or other immunoassay techniques.
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2 protocols using apc rat anti trem2 antibodies
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Microglia Isolation and Characterization
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Microglia Isolation and Characterization
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Microglia were isolated from the hippocampus as described above and blocked with rat- anti CD16/CD32 Fc antibodies 1:100 (Cat# 553142, BD Biosciences) and then stained with rat-anti CD11b-PeCy7 1:100 (Cat# 25-0112-82, ebiosciences), rat-anti CD45-PeCPCy5.5 antibodies 1:100 (Cat# 45-0451-82, ebiosciences), APC-rat anti Trem2 antibodies 1:10 (Cat# FAB17291A R & D systems), or APC-mouse anti CX3CR1 antibodies 1:100 (Cat# 149008) for 25 min on ice. Labeled cells were washed with 3 ml of cold FACS buffer, spun as above, resuspended in 300ul of cold FACS buffer and assessed for antigen expression using a BD LSR fortessa FACS (BD Biosciences). Data were analyzed using FlowJo software (Tree Star) and gating for each antibody was determined with isotype-stained control.
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