Western blots were performed as described [8 (link)] by using 10μg total extracts per lane and antibodies against BCL6 (N3 #sc-858, Santa Cruz, Heidelberg, Germany); STAT6 (M-200 #sc-1698, Santa Cruz); β-Actin (Sigma).
N3 sc 858
Manufactured by Santa Cruz Biotechnology
Sourced in Germany
The N3 #sc-858 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for specific scientific applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using n3 sc 858
1
Immunoblotting for BCL6, STAT6, and β-Actin
2
Double Immunostaining of Hyperplastic Tonsils
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sequential double immunostaining was performed on TMA slides containing hyperplastic tonsils. Briefly, paraffin sections were cut, pretreated, and immunostained with the internal region-specific MEF2B antibody or with a polyclonal rabbit BCL6 antibody (N-3, sc-858, Santa Cruz), as outlined above. Subsequently, the slides were incubated with monoclonal mouse anti-PD1 antibody (NAT105C, kindly provided by Giovanna Roncador, Monoclonal antibodies Core Unit, Spanish National Cancer Centre, Madrid, Spain) which was detected with an antimouse alkaline phosphatase micropolymer and AP-Red chromogen (both from Histopathology Ltd., Pécs, Hungary).
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